Janelia Fluor® Dyes | 형광 Dyes | 고마바이오텍

• Janelia Fluor®(JF) 형광 dye는 초고해상도 현미경을 포함한 광범위한 응용 분야에 이용되며, 밝고 빛에 안정하며 세포투과성을 갖고 있습니다.
  
• 다양한 biomolecule과의 conjugation을 위해 reactive group을 선택할 수 있습니다. 
• 장시간 형광을 유지합니다. 

적용

• Suitable for use in confocal microscopy, IHC, ICC and flow cytometry
• Especially well-suited to live-cell imaging
  
• Ideal for super-resolution techniques including STED and dSTORM
• Photoactivatable Janelia Fluor® dyes are compatible with PALM microscopy

주요 제품 

참고 데이터

Figure 1: Application of Janelia Fluor® Dyes in Cardiac Tissue. Super-resolution image (dSTORM) of muscle tissue from the left ventricle of an adult pig heart. Left: JF 646 (Cat. No. 6148) was used to label collagen VI, which is present on the cell plasma membrane, and imaged with a 642 nm excitation laser. A clearer structure is achieved in comparison with the widefield image (top right). Scale bar 2 μm. Right: - Displays the distribution of collagen VI in the interstitial space between muscle cells. Labeled with a primary ab against collagen VI and a secondary ab conjugated to JF 549 (Cat. No. 6147). Scale: 50 μm. / Images kindly provided by Prof. Christian Soeller, University of Exeter; acquired by Alex Clowsley and Anna Meletiou.

Figure 2: Left: Application of Janelia Fluor® Probes Left: COS7 cells were labeled with 3 μM Taxol Janelia Fluor® 646 (Cat. No. 6266) for one hour at 37°C. Images were taken on a Leica TCS SP8 Confocal Laser Scanning Microscope. Image kindly provided by Prof. Christian Soeller, University of Exeter; acquired by Evelina Lucinskaite, Anna Meletiou and Alexander Clowsley. Middle: Immortalized human keratinocyte cells expressing a GFP-tagged α4 integrin were treated with 10 μM BOP-Janelia Fluor® 646 (Cat. No. 6997). Cells were incubated at 37°C and imaged using a Nikon A1R confocal microscope. Scale bar = 10 μm. Right: Immortalized human keratinocytes expressing a GFP-tagged α4 integrin were incubated with 10 μm BOP-Janelia Fluor® 549 (Cat. No. 6996). Cells were incubated at 37°C and imaged using Nikon A1R confocal microscope. Scale bar = 10 μm. Middle and right images kindly provided by Willow Hight-Warburton, Kings College London.