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제품 상세

Antibody

6.5 - 270 kDa 범위를 커버하는 3가지 컬러 밴드를 포함하며,
최대 100%까지의 transfer 효과를 개런티하는 Prestained protein ladder를 소개합니다.

특징

 

  • iPSC-derived human cardiomyocyte는 심부전, 심장독성 테스트,  약물 유효성 확인, 신진대사 연구, 전기생리학 실험 등에 사용됩니다.
  • 해동되면 in vitro에서 자발적으로 박동하기 시작하며, troponin을 포함한 심장 특이 마커를 발현합니다. 
  • Ventricular cardiomyocyte (심실심근세포)와 Atrial cardiomyocyte (심방심근세포) 및 culture media를 제공합니다. 
  • CiPA-validated되어 약물 평가 및 cardiotoxicity 스크리닝에 사용됩니다. 

  


사양


Starting material Fibroblasts
Donor gender Male
Donor age at sampling 74 yrs
Reprogramming method Episomal vector
Induction method Fully defined medium
Size 1 million cells
Growth properties Adherent
Disease information Healthy


 

적용 

 

  • Electrophysiology : Contractility, QT prolongation, Na+ & Ca2+ channels, Pharmacology 실험
  • Functional Integrity : Ca2+ signaling, Morphology, Stress & toxic response markers 확인

   


제품

Catalog. No. Product Name Format Conc.
ax2508 Human iPSC-Derived Ventricular Cardiomyocytes (Male) 1.0 million cells/vial
ax2518 Human iPSC-Derived Atrial Cardiomyocytes (Male) 1.0 million cells/vial
ax2530-500 Cardiomyocyte Maintenance Medium 1 x 500 mL Basal Medium 1 x 10 mL Supplement
ax0049 Fibronectin Coating Solution 1 mL
ax2500 Human iPSC-Derived Ventricular Cardiomyocyte Kit  Cell (ax2508) + Media (ax2530-500) + Fibronectin (ax0049)



참고 데이터

1. Gene expression : Pan-cardiac, atrial, ventricular marker genes의 발현 비교



2. Immunohistochemistry : Cardiomyocyte 특이적인 marker를 발현합니다.


Axol Human iPSC-Derived Ventricular Cardiomyocytes were cultured in Cardiomyocyte Maintenance Medium prior to being fixed and stained for the markers Sarcomeric Alpha Actinin and Cardiac Troponin. The cells were counterstained using DAPI to allow visualization of nuclei.



Expression of Troponin T, atrial natriuretic peptide (ANP) and atrial myosin light chain 2 (MLC2a) was confirmed in Axol iPSC-Derived Atrial Cardiomyocytes using immunocytochemistry. Troponin T staining (red) confirmed the presence of cardiac myocytes which are responsible for sarcomere contraction. ANP is specifically secreted by atrial myocytes upon atrial stretching and MLC2a facilitates cardiac contractility wo. DAPI counterstain.  


3. Beating : 배양 후 자발적으로 박동합니다.




4. Action potential characteristics : patch clamp에서 자발적인 action potential을 보여줍니다.


Characteristics of spontaneous and evoked action potentials recorded from Axol’s Human iPSC-derived Ventricular Cardiomyocytes. Spontaneous (A) and evoked (1 Hz; B) action potentials recorded by whole-cell patch clamp. (C) Average action potential parameters for spontaneous and evoked action potentials in control conditions.


5. Core cardiac ion channel pharmacology : INa, ICa,L and IKr을 발현합니다.


Axol’s Human iPSC-derived Ventricular Cardiomyocytes express the core cardiac ion channels INa, ICa,L and IKr. (A) Representative traces of evoked action potentials recorded under control conditions (grey) and in the presence of 100 μM Lidocaine (green), 100 nM Nifedipine (blue) or 50 nM Dofetilide (red), which show expected effects on action potential amplitude and duration. B) Average effect of each compound on spontaneous action potential parameters, presented as percent of control ± SEM, N ≥ 4. Statistical significance calculated by a paired two-tailed Student’s t-test (* p<0.05, ** p<0.01, *** p<0.001).


6. Modeling Arrhythmias : 부정맥 분석에 사용할 수 있습니다.


Axol’s Human iPSC-Derived Ventricular Cardiomyocytes were treated with doxorubicin (control, 500 nM, 100 nM and 50 nM). There was a dose-dependent response at 13 h and 24 h post-treatment with doxorubicin. Arrhythmias and loss of beating amplitude were seen at lower doxorubicin concentration, whereas higher doxorubicin concentrations resulted in tachycardia and ultimately cell death. Data kindly provided by Dr J. Maynes, University of Toronto.


7. CiPA(Comprehensive in vitro Pro-Arrhythmia Assay) ​: 약물의 심장독성 확인에 이용됩니다.


Na+와 K+를 block하는 Quinidine을 테스트. Axol의 Cardiomyocyte는 낮은 농도에서 triangulation의 증가를 확인


Anti Dopaminergic Drug인 Droperidol을 처리하여, 가장 낮은 농도에서 QT prolongation을 확인



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0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
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