AXOL 심실심근세포 | Ventricular Cardiomyocyte | Cardiac cells | 고마바이오텍

특징

• iPSC-derived human ventricular cardiomyocyte는 심부전, 심장독성 테스트,  약물 유효성 확인, 신진대사 연구, 전기생리학 실험 등에 사용됩니다.
• 해동 후 3일 안에 in vitro에서 자발적으로 박동하기 시작하며, 7일안에 assay에 사용할 수 있습니다.
• Troponin을 포함한 심장 특이 마커를 발현합니다. 
• 28가지 CiPA compound에 validation되었으며, 약물 평가 및 cardiotoxicity 스크리닝에 사용됩니다. 
  

사양

적용 

• Electrophysiology : Contractility, QT prolongation, Na+ & Ca2+ channels, Pharmacology 실험
• Functional Integrity : Ca2+ signaling, Morphology, Stress & toxic response markers 확인

Axol Human iPSC-Derived Ventricular Cardiomyocytes were cultured in Cardiomyocyte Maintenance Medium prior to being fixed and stained for the markers Sarcomeric Alpha Actinin and Cardiac Troponin. The cells were counterstained using DAPI to allow visualization of nuclei.

Expression of Troponin T, atrial natriuretic peptide (ANP) and atrial myosin light chain 2 (MLC2a) was confirmed in Axol iPSC-Derived Atrial Cardiomyocytes using immunocytochemistry. Troponin T staining (red) confirmed the presence of cardiac myocytes which are responsible for sarcomere contraction. ANP is specifically secreted by atrial myocytes upon atrial stretching and MLC2a facilitates cardiac contractility wo. DAPI counterstain.  

Characteristics of spontaneous and evoked action potentials recorded from Axol’s Human iPSC-derived Ventricular Cardiomyocytes. Spontaneous (A) and evoked (1 Hz; B) action potentials recorded by whole-cell patch clamp. (C) Average action potential parameters for spontaneous and evoked action potentials in control conditions.

Axol’s Human iPSC-derived Ventricular Cardiomyocytes express the core cardiac ion channels INa, ICa,L and IKr. (A) Representative traces of evoked action potentials recorded under control conditions (grey) and in the presence of 100 μM Lidocaine (green), 100 nM Nifedipine (blue) or 50 nM Dofetilide (red), which show expected effects on action potential amplitude and duration. B) Average effect of each compound on spontaneous action potential parameters, presented as percent of control ± SEM, N ≥ 4. Statistical significance calculated by a paired two-tailed Student’s t-test (* p<0.05, ** p<0.01, *** p<0.001).

Axol’s Human iPSC-Derived Ventricular Cardiomyocytes were treated with doxorubicin (control, 500 nM, 100 nM and 50 nM). There was a dose-dependent response at 13 h and 24 h post-treatment with doxorubicin. Arrhythmias and loss of beating amplitude were seen at lower doxorubicin concentration, whereas higher doxorubicin concentrations resulted in tachycardia and ultimately cell death. Data kindly provided by Dr J. Maynes, University of Toronto.

Na+와 K+를 block하는 Quinidine을 테스트. Axol의 Cardiomyocyte는 낮은 농도에서 triangulation의 증가를 확인

Anti Dopaminergic Drug인 Droperidol을 처리하여, 가장 낮은 농도에서 QT prolongation을 확인