| Catalog
No. |
Product Name |
Format Conc. |
| ax2510 |
Human iPSC-Derived Atrial
Cardiomyocyte Kit |
Cell (ax2518) + Media (ax2530-500) +
Fibronectin (ax0049) |
| ax2518 |
Human iPSC-Derived Atrial
Cardiomyocyte |
1.0 million cells/vial |
| ax2530-500 |
Cardiomyocyte Maintenance Medium |
1 x 500 mL Basal Medium 1 x 10 mL
Supplement |
| ax0050 |
Fibronectin Coating Solution |
1 mL |
참고 데이터
1) Phenotypic characterization: ICC
Immunocytochemistry data of cardiac- and atrial-specific proteins. Troponin T staining (red) confirmed the presence of cardiac myocytes, ANP is specifically secreted by atrial myocytes upon atrial stretching and MLC2a facilitates cardiac contractility. The nuclear marker DAPI was used as a counterstain.
2) Phenotypic characterization: RNAseq
RNASeq characterization of axoCells human iPSC-derived ventricular (ax2508) and atrial (ax2518) cardiomyocytes. axoCells cardiomyocytes grown for 14 days compared to iPSCs from the same donor. Heatmap displays expression of several key cardiac markers in axoCells atrial and ventricular cardiomyocytes with respective Z-scores (White – high expression; Black – low expression)
3) Functional relevance: compound testing on patch clamp
Atrial cardiomyocyte phenotype was confirmed by the modulation of spontaneous action potentials using selective pharmacological compounds. Work done in collaboration with Metrion Biosciences.
Representative spontaneous action potentials are shown under control conditions (grey) and after the application of 50 μM 4-AP (A, blue) or 1 μM Carbachol (B, orange). The bar graphs show the average effect (% of control) on action potential parameters (N=5). (A) A low concentration of 4-aminopyridine (4-AP) selectively inhibits the IKur current to broaden action potential duration and slow spontaneous firing. (B) Carbachol was used to activate the IKACh current, resulting in action potential shortening and profound slowing of firing frequency.
4) Functional relevance: contractility
The effect of carbachol, an activator of IKAch in atrial cardiomyocytes, on the mean beat rate of axoCells atrial (left) and ventricular (right) hiPSC-CMs, as measured on the Flexcyte 96.
관련 자료
Protocol