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시약 및 기기 상세
유전자 도입을 위한 non-viral TcBuster mRNA Transposase & Transposon 이미지

유전자 도입을 위한 non-viral TcBuster mRNA Transposase & Transposon

Maker : BIO-TECHNE

TcBuster 시스템은 바이러스를 사용하지 않고 유전자를 세포내로 전달하는 방법으로, 유전자 전달 효율이 높고 안전하여, 세포치료제 개발에 효율적으로 적용될 수 있습니다.

프린트 제품 문의
특징

  • TcBuster는 여러 개의 관심 유전자를 전이하고 안정적으로 통합할 수 있는 대용량 전이소 시스템입니다. 
  • 바이러스 벡터에 대한 효과적인 대안인 TcBuster는 CAR T 또는 CAR NK와 같은 세포 치료제 개발 과정에서 상당한 시간과 비용 절감을 제공합니다.
  •  바이러스 벡터 기반의 공학(예: 렌티바이러스 또는 AAV)이 세포에 DNA 화물을 전달하는 표준 방법이었지만, TcBuster 시스템은 표준 전기천공 방법을 사용하여 비바이러스 솔루션을 제공합니다.

 

 TcBuster Reagent


TcBuster 제품군
mRNA Transposase TCB-001.1-100 (100 µg)  100 tests at 1 µg each - Useful for system evaluation
TCB-001.1-500 (500 µg) 500 tests at 1 µg each - Useful for further development
TCB-001.1-GMP (500 µg) 500 tests at 1 µg each - GMP grade
- Useful for pre-IND process development and ex vivo clinical applications
TcBuster-Compatible Transposons TCBP001-100 (100 µg) 25-100 tests at 1-4 µg each - Multicistronic CAR-containing transposon
- Useful in cell therapy applications as an experimental control
TCBP002-100 (100 µg) 25-100 tests at 1-4 µg each - Whole plasmid insertion of eGFP with no untransposed plasmid expression
- Useful in bioprocessing system evaluation as an experimental control
Custom Transposons 　 - for process development using your own therapeutic cargo
- Utilizes partnership with Aldevron™
Anti-TcBuster-M Transposase Antibody      MAB11511 (25 µg, 100 µg) 1 µg/mL for Western Blot - Primary antibody detects TcBuster-M transposase enzyme in Western blotting applications
20 µg/mL for Simple Western

  

Mechanistic Diagram of the TcBuster Non-Viral Gene Editing System.

The TcBuster system components are introduced into cells via electroporation (1). The TcBuster-M transposase mRNA is then translated into the transposase enzyme (2), which binds to the inverted terminal repeats (ITRs) on the DNA transposon (3). The transposase enzyme excises the genes of interest (GOI) from the transposon (4) and inserts them into the host genome (5). The GOI mRNA is transcribed from the host genome (6), and the protein is stably expressed in the edited cells (7).




Efficient gene editing of T cells modified with different sized cargos on different electroporation platforms.

Primary T cells from 3 donors were expanded for 7 days after genetic modification in GMP Human T Cell Media (Catalog # CCM038-GMP-1L) supplemented with 5% hAB serum and 10 ng/mL each of IL-7 (Catalog # BT-007-GMP) and IL-15 (Catalog # BT-015-GMP) in a 6 well G-Rex®. (A). Representative flow plots of genetically modified T cells with a 5.1 kb insert containing a GFP sequence, introduced on the ThermoFisher Neon™, Lonza 4D- Nucleofector®, or MaxCyte®. (B).Three DNA cargos introduced on the same electroporation platforms achieve high editing efficiency in T cells (>40%). Data points represent the average of 3 donors with technical duplicates ± SD.



TcBuster edited CD19-CAR-T cells specifically kill CD19+ target cells at low E:T ratios.

T cells from 3 donors were expanded for 7 days after genetic modification in GMP Human T Cell Media (Catalog # CCM038-GMP-1L) supplemented with 5% hAB serum and 10 ng/mL each of IL-7 (Catalog # BT-007-GMP) and IL-15 (Catalog # BT-015-GMP) in a 6 well G-Rex®. (A) TcBuster efficiently integrates cargos of different sizes regardless of electroporation platform, resulting in a similar number of total modified T cells produced. (B) T cells from 3 donors modified with the 5.1 kb plasmid were cryopreserved in CS10 following 7 days of culture after electroporation. T cells were thawed and immediately added to either CD19+ or CD19- target cells at different E:T ratios and controlled target cell growth after 24 hours of co-culture.



Genetic characterization of TcBuster genetically modified primary T cells shows a favorable insertional profile and low copy number.

(A) T cells from 3 donors were collected after 9 days of culture for digital PCR analysis of the population’s average adjusted vector copy number (VCN). (B) Integration site analysis (ISA) was performed on 3 different T cell donors that were modified with a different range of plasmid sizes with TcBuster or a lentiviral vector. DNA libraries were Illumina sequenced by GeneWerk (now ProtaGene).




TcBuster edits primary T cells as efficiently or better than lentivirus.

T cells from 3 donors were expanded for 9 days in GMP Human T Cell Media, supplemented with 5% hAB serum and 10 ng/mL each of IL-7 and IL-15, in a 6 well G-Rex®. The GOI is refers to the promoter and genes integrated in the genome. Graph shows the efficiency of genetic modification for both TcBuster and lentivirus, with TcBuster showing comparable or better editing efficiency. Data points represent the average of 3 donors ± SD.

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
TcBuster-M Transposase Antibody MAB11511 25 ug, 100 ug BIOTECHNE
TcBuster-M™ Transposase mRNA TCB-001.1-100 100 µg BIOTECHNE
TcBuster™ Transposon CD19CAR-DHFR-eGFP TCBP001-100 100 µg BIOTECHNE
TcBuster™ Transposon Insert On eGFP TCBP002-100 100 µg BIOTECHNE
TcBuster™-M Transposase mRNA TCB-001.1-500 500 µg BIOTECHNE
Maker
BIOTECHNE
Cat.No.
MAB11511
Product
TcBuster-M Transposase Antibody
Size
25 ug, 100 ug
Qty
Data Sheet
MSDS
Maker
BIOTECHNE
Cat.No.
TCB-001.1-100
Product
TcBuster-M™ Transposase mRNA
Size
100 µg
Qty
Data Sheet
MSDS
Maker
BIOTECHNE
Cat.No.
TCBP001-100
Product
TcBuster™ Transposon CD19CAR-DHFR-eGFP
Size
100 µg
Qty
Data Sheet
MSDS
Maker
BIOTECHNE
Cat.No.
TCBP002-100
Product
TcBuster™ Transposon Insert On eGFP
Size
100 µg
Qty
Data Sheet
MSDS
Maker
BIOTECHNE
Cat.No.
TCB-001.1-500
Product
TcBuster™-M Transposase mRNA
Size
500 µg
Qty
Data Sheet
MSDS

추천제품

관련제품

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS

자료

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제품 문의

 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
Maker
Cat.No.
Product
Size
Qty
Data Sheet
MSDS

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