조혈모세포 CFC 분석을 위한 Methylcellulose media | 고마바이오텍

특징

• Hematopoietic progenitor의 lineage를 정량화하는데 이용되는 Colony forming cell (CFC) assay는 시간이 많이 걸리고 쉽지 않을 수 있습니다. 이러한 Cell colony의 성공적인 계수는 정확한 세포수, 성장인자 및 cytokine의 존재, 적절한 습도 및 고품질 배지 등과 같은 요인에 달려있습니다. R&D Systems의 Methylcellular media는 CFC 분석에 최적화된 구성 요소를 제공합니다. 
• CFC 분석은 조혈 전구 세포가 사이토카인 자극에 반응하여 반고체 배지에서 콜로니로 증식하고 분화하는 능력에 기초합니다. 형성된 콜로니는 고유한 형태에 따라 열거하고 특성화할 수 있습니다.

주요 제품

▒ Methylcellulose Stock and Base Media  

▒ Complete Methylcellulose Media  

데이터

Human Hematopoietic Colony Formation Using the Methylcellulose-based Colony Forming Cell Assay. 

Colony forming unit-erythroid (CFU-E) are clonogenic progenitors that produce only one or two clusters with each cluster containing from 8 to approximately 100 hemoglobinized erythroblasts. It represents the more mature erythroid progenitors that have less proliferative capacity.B. Colony forming unit-granulocyte (CFU-G) are clonogenic progenitors of granulocytes that give rise to a homogeneous population of eosinophils, basophils, or neutrophils.C. Colony forming unit-granulocyte, macrophage (CFU-GM) are progenitors that give rise to colonies containing a heterogeneous population of macrophages and granulocytes. The morphology is similar to the CFU-M and CFU-G descriptions.D. Burst forming unit-erythroid (BFU-E) colonies can be described as small (3 to 8 clusters), intermediate (9 to 16 clusters), or large (more than 16 clusters) according to the number of clusters present. These are primitive erythroid progenitors that have high proliferative capacity.E. Colony forming unit-macrophage (CFU-M) are clonogenic progenitors of macrophages that give rise to a homogenous population of macrophages.F. Colony forming unit-granulocyte, erythrocyte, macrophage, megakaryocyte (CFU-GEMM) are multi-lineage progenitors that give rise to erythroid, granulocyte, macrophage and megakaryocyte lineages, as the name indicates.

Mouse Hematopoietic Colony Formation Using the Methylcellulose-based Colony Forming Cell Assay. 

Burst forming unit-erythroid (BFU-E) colonies are defined as clusters with a minimal of 30 cells that can be seen from day 7 onward. Each individual cluster consisted of tiny, irregular shaped cells that may appear fused together. Each cluster normally contains 5-8 cells, and the size of the cluster is similar to that of a single macrophage. The cluster may vary in sizes and color. A large BFU-E is usually bright red and is differentiable even without the use of a microscope. Smaller BFU-E may not appear red in color but is distinguishable based on the morphology.B.Colony forming unit-macrophage (CFU-M; left) are clonogenic progenitors of macrophages that give rise to a homogenous population of macrophages. Colony forming unit-granulocyte (CFU-G; right) are clonogenic progenitors of granulocytes that give rise to a homogeneous population of eosinophils, basophils, or neutrophils.C. Colony forming unit-granulocyte, macrophage (CFU-GM) are progenitors that give rise to colonies containing a heterogeneous population of macrophages and granulocytes. The morphology is similar to the CFU-M and CFU-G descriptions.D.Colony forming unit-granulocyte, erythrocyte, macrophage, megakaryocyte (CFU-GEMM) are multi-lineage progenitors that give rise to the lineage of erythroid, granulocytes, macrophages, and megakaryocytes as the name indicates. It can be identified as reddish colored cells (erythroid) mixed with colorless cells (granulocytes, macrophages, and megakaryocytes) in a single colony. This progenitor is typically the largest colony on the culture dish; occasionally CFU-GM may attain a size comparable or larger than that of CFU-GEMM.