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제품 상세
유전독성 시험을 위한 Comet Assay Kits 이미지

유전독성 시험을 위한 Comet Assay Kits

OxiSelect™ Comet Assay Kit는 Single Cell Gel Electrophoresis assay를 통해 세포의 DNA 손상을 측정하는 Assay Kit 입니다.

프린트 제품 문의

특징

 

  • 다양한 형식의 DNA 손상에 대해 Single cell gel electrophoresis assay 방법으로 스크리닝합니다.
  • 전류가 흐르는 동안 손상된 DNA는 "comet (혜성)" 모양을 형성하며, Epifluorescence microscopy로 확인한 결과 건강한 DNA와 시각적으로 구분됩니다.
  • 96-well 혹은 3-well 형식으로 제공됩니다.
  • low-melting agarose의 경우 Slide 형식을 이용하여 분석하실 수 있습니다.


실험 과정 





실험 결과


Etoposide Treatment of Jurkat Cells. Jurkat cells untreated (left) and treated (right) with Etoposide before performing OxiSelect™ Comet Assay under 
alkaline electrophoresis conditions at 33V / 300 mA for 15 minutes.



Epifluorescence Microscopy Visualization of DNA Damage using the OxiSelect™ Comet Assay Kit. 



사용 논문

  1. Park, K. et al. (2021). Aicardi-Goutières syndrome-associated gene SAMHD1 preserves genome integrity by preventing R-loop formation at transcription–replication conflict regions. PLoS Genet. 17(4): e1009523. doi: 10.1371/journal.pgen.1009523.
  2. Ramos, H. et al. (2021). A selective p53 activator and anticancer agent to improve colorectal cancer therapy. Cell Rep. 35(2):108982. doi: 10.1016/j.celrep.2021.108982.
  3. Planelló, R. et al. (2021). Genotoxic effects and transcriptional deregulation of genetic biomarkers in Chironomus riparius larvae exposed to hydroxyl- and amine-terminated generation 3 (G3) polyamidoamine (PAMAM) dendrimers. Sci Total Environ. doi: 10.1016/j.scitotenv.2021.145828.
  4. Fan, D. et al. (2021). A Novel Salt Inducible Kinase 2 Inhibitor, ARN-3261, Sensitizes Ovarian Cancer Cell Lines and Xenografts to Carboplatin. Cancers (Basel). 13(3):446. doi: 10.3390/cancers13030446.
  5. Siemionow, M. et al. (2020). Transplantation of Dystrophin Expressing Chimeric (DEC) Human Cells of Myoblast/MSC Origin Improves Function in Duchenne Muscular Dystrophy Model. Stem Cells Dev. doi: 10.1089/scd.2020.0161.
  6. Lammert, C.R. et al. (2020). AIM2 inflammasome surveillance of DNA damage shapes neurodevelopment. Nature. doi: 10.1038/s41586-020-2174-3.
  7. Shibayama, Y. et al. (2020). Aberrant (pro)renin receptor expression induces genomic instability in pancreatic ductal adenocarcinoma through upregulation of SMARCA5/SNF2H. Commun Biol. 3(1):724. doi: 10.1038/s42003-020-01434-x.
  8. Han, J. et al. (2020). Elevated CXorf67 Expression in PFA Ependymomas Suppresses DNA Repair and Sensitizes to PARP Inhibitors. Cancer Cell. doi: 10.1016/j.ccell.2020.10.009.
  9. Hays, E. et al. (2020). The SWI/SNF ATPase BRG1 stimulates DNA end resection and homologous recombination by reducing nucleosome density at DNA double strand breaks and by promoting the recruitment of the CtIP nuclease. Cell Cycle. doi: 10.1080/15384101.2020.1831256.
  10. Ibnu Rasid, E.N. et al. (2020). Effect of Dioscorea hispida var. Daemona (Roxb) Prain & Burkill on Oxidative Stress and DNA Damage in the Liver of Pregnant Rats. Int J Biomed Sci. 16(3).
  11. Le, B.V. et al. (2020). TGFβR-SMAD3 Signaling Induces Resistance to PARP Inhibitors in the Bone Marrow Microenvironment. Cell Rep. 33(1):108221. doi: 10.1016/j.celrep.2020.108221.
  12. Klotz-Noack, K. et al. (2020). SFPQ Depletion Is Synthetically Lethal with BRAFV600E in Colorectal Cancer Cells. Cell Rep. 32(12):108184. doi: 10.1016/j.celrep.2020.108184.
  13. Ito, S.S. et al. (2020). Inhibition of the ATR kinase enhances 5-FU sensitivity independently of non-homologous end-joining and homologous recombination repair pathways. J Biol Chem. doi: 10.1074/jbc.RA120.013726.
  14. Klak, M. et al. (2020). Irradiation with 365 nm and 405 nm wavelength shows differences in DNA damage of swine pancreatic islets. PLoS One. 15(6):e0235052. doi: 10.1371/journal.pone.0235052.
  15. Khalil, A.M. et al. (2020).  Association between Mobile Phone Using and DNA Damage of Epithelial Cells of the Oral Mucosa. J Biotechnol Biomed. 3(2020): 50-66. doi: 10.26502/jbb.2642-91280027.
  16. Wang, Y. et al. (2020). Targeting therapeutic vulnerabilities with PARP inhibition and radiation in IDH-mutant gliomas and cholangiocarcinomas. Sci Adv. doi: 10.1126/sciadv.aaz3221.
  17. Fang, Y. et al. (2020). Epigenetic dysregulation of Mdr1b in the blood-testis barrier contributes to dyszoospermia in mice exposed to cadmium. Ecotoxicol Environ Saf. 190:110142. doi: 10.1016/j.ecoenv.2019.110142.
  18. Cupello, S. et al. (2019). Distinct roles of XRCC1 in genome integrity in Xenopus egg extracts. Biochem J. 476(24):3791-3804. doi: 10.1042/BCJ20190798.
  19. Naci, D. et al. (2019). Cell adhesion to collagen promotes leukemia resistance to doxorubicin by reducing DNA damage through the inhibition of Rac1 activation. Sci Rep. 9(1):19455. doi: 10.1038/s41598-019-55934-w.
  20. Lu, S. et al. (2019). Additive effects of a small molecular PCNA inhibitor PCNA-I1S and DNA damaging agents on growth inhibition and DNA damage in prostate and lung cancer cells. PLoS One. 14(10):e0223894. doi: 10.1371/journal.pone.0223894.
  21. Dedobbeleer, M. et al. (2019). MKP1 phosphatase is recruited by CXCL12 in Glioblastoma cells and plays a role in DNA Strand Breaks Repair. Carcinogenesis. doi: 10.1093/carcin/bgz151.
  22. Zhou, J. et al (2019). Aberrantly Expressed Timeless Regulates Cell Proliferation and Cisplatin Efficacy in Cervical Cancer. Hum Gene Ther. doi: 10.1089/hum.2019.080.
  23. Sarkar, R. et al. (2019). Rotavirus activates a noncanonical ATM-Chk2 branch of DNA damage response during infection to positively regulate viroplasm dynamics. Cell Microbiol. doi: 10.1111/cmi.13149.
  24. Maner, J. et al. (2019). Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions. RSC Adv. 9:39447-39457. doi: 10.1039/C9RA05352B.
  25. Han, Y. et al. (2019). Exposure to waterborne nTiO2 reduces fertilization success and increases polyspermy in a bivalve mollusc: A threat to population recruitment. Environ Sci Technol. doi: 10.1021/acs.est.9b03675.
  26. Shansky, Y.D. et al. (2019). Human Platelet Lysate Sustains the Osteogenic/Adipogenic Differentiation Potential of Adipose-Derived Mesenchymal Stromal Cells and Maintains Their DNA Integrity in vitro. Cells Tissues Organs. doi: 10.1159/000502813.
  27. Gothai, S. et al. (2019). In Vitro and In Vivo-Scientific Evaluation on Cytotoxicity and Genotoxicity of Traditional Medicinal Plant Couroupita Guianensis Aubl. Flower. PhOL. 2:24-38. 
  28. Al Khateeb, W. et al. (2019). Growth, Yield and Genetic Integrity of Spinach and Chrysanthemum as Affected by Soil Supplementation with Dam Sediments Collected From King Talal and Al-Mujib Dams/ Jordan. World Appl. Sci. J. 37(1):58-69. doi: 10.5829/idosi.wasj.2019.58.69.
  29. Su, J. et al. (2019). Genomic Integrity Safeguards Self-Renewal in Embryonic Stem Cells. Cell Rep. 28(6):1400-1409.e4. doi: 10.1016/j.celrep.2019.07.011.
  30. Xia, Y. et al. (2019). Rescue of DNA damage after constricted migration reveals a mechano-regulated threshold for cell cycle. J Cell Biol. pii: jcb.201811100. doi: 10.1083/jcb.201811100.

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
OxiSelect™ 10-Well Comet Assay Slides STA-853 25 slides CELL BIOLABS
OxiSelect™ 10-Well Comet Assay Slides STA-852 5 slides CELL BIOLABS
OxiSelect™ 96-Well Comet Assay Kit STA-355 96 assays CELL BIOLABS
OxiSelect™ 96-Well Comet Assay Kit STA-355-5 5x96 assays CELL BIOLABS
OxiSelect™ 96-Well Comet Assay Slide STA-356-5 5 slides CELL BIOLABS
OxiSelect™ 96-Well Comet Assay Slide STA-356 1 slide CELL BIOLABS
OxiSelect™ Comet Assay Kit (10-Well Slides) STA-851 250 assays CELL BIOLABS
OxiSelect™ Comet Assay Kit (10-Well Slides) STA-850 50 assays CELL BIOLABS
OxiSelect™ Comet Assay Kit (3-Well Slides) STA-351 75 assays CELL BIOLABS
OxiSelect™ Comet Assay Kit (3-Well Slides) STA-350 15 assays CELL BIOLABS
OxiSelect™ Comet Assay Kit (3-Well Slides) STA-351-5 5x75 assays CELL BIOLABS
OxiSelect™ Comet Assay Slides (3-Well) STA-353 25 slides CELL BIOLABS
OxiSelect™ Comet Assay Slides (3-Well) STA-352 5 slides CELL BIOLABS
OxiSelect™ Comet Assay Slides (3-Well) STA-353-5 5x25 slides CELL BIOLABS
전체보기
Maker
CELL BIOLABS
Cat.No.
STA-853
Product
OxiSelect™ 10-Well Comet Assay Slides
Size
25 slides
Qty
Data Sheet
Maker
CELL BIOLABS
Cat.No.
STA-852
Product
OxiSelect™ 10-Well Comet Assay Slides
Size
5 slides
Qty
Data Sheet
Maker
CELL BIOLABS
Cat.No.
STA-355
Product
OxiSelect™ 96-Well Comet Assay Kit
Size
96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-355-5
Product
OxiSelect™ 96-Well Comet Assay Kit
Size
5x96 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-356-5
Product
OxiSelect™ 96-Well Comet Assay Slide
Size
5 slides
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-356
Product
OxiSelect™ 96-Well Comet Assay Slide
Size
1 slide
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-851
Product
OxiSelect™ Comet Assay Kit (10-Well Slides)
Size
250 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-850
Product
OxiSelect™ Comet Assay Kit (10-Well Slides)
Size
50 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-351
Product
OxiSelect™ Comet Assay Kit (3-Well Slides)
Size
75 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-350
Product
OxiSelect™ Comet Assay Kit (3-Well Slides)
Size
15 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-351-5
Product
OxiSelect™ Comet Assay Kit (3-Well Slides)
Size
5x75 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-353
Product
OxiSelect™ Comet Assay Slides (3-Well)
Size
25 slides
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-352
Product
OxiSelect™ Comet Assay Slides (3-Well)
Size
5 slides
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-353-5
Product
OxiSelect™ Comet Assay Slides (3-Well)
Size
5x25 slides
Qty
Data Sheet
MSDS
전체보기

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주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS

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