mRNA 항원을 이용한 막단백질 항체 제작 서비스

제품 상세

mRNA 항원을 이용한 antibody service로 에피토프 접근이 어려운 transmembrane protein에 대한 항체를 효과적으로 생산할 수 있습니다.

프린트 제품 문의

특징

항원별 비교

Immunogen format	Weaknesses	Strengths	Recommended index
mRNA	Instability of mRNA, mRNA delivery	Direct translation of mRNA in the cytoplasm, spontaneous protein PTM in mammalian cells, natural conformation, no need for adjuvant	★★★★★
DNA	low expression level since DNA must cross the nuclear membrane for transcription, high cost for DNA immunization		★
Protein	High technical limitation of full length GPCRs expression and purification in vitro	High immunogenicity	★★
Peptide	Only recognize linear epitopes, not conformational epitopes, epitope omission	Low synthesis cost	★★★★
VLP	Non-specific antibody generation, little difference between positive and negative screening	Higher abundance of target antigen compared with overexpressed cells	★★★
Overexpressed cell line	Low immunogenicity due to the very low percentage of GPCR of interest on the entire membrane		★★
Nanodisc	Low success rate of GPCRs assembly with MSPs and phospholipid to form Nanodisc	Similar to natural cell membrane structure	★★★

Workflow

Case Study

Case 1) 7TM protein hCCR9

Goal: Generate mouse monoclonal antibodies specific to 7-transmembrane protein hCCR9
Immunogen: hCCR9-mRNA-LNP
Screening Material: hCCR9-overexpressing CHO-K1
Challenges: No available purified protein & failed in DNA immunization
Results: Successfully generate 9 hCCR9-positive clones and 7 validated clones (client selected)

Case 2) 4TM protein hCLD-18.2

Goal: Generate mouse monoclonal antibodies specific to 4-transmembrane protein hCLD-18.2
Immunogen: hCLD18.2-mRNA-LNP
Screening Material: hCLD18.2-overexpressing CT26
Challenges: No available purified protein & failed in DNA immunization
Results: Successfully generate 386 hCLD18.2-positive parental clones

Case 3) 7TM protein hPTX

Goal: Generate mouse monoclonal antibodies that are specific to 7-transmembrane protein hPTX
Immunogen: hPTX-mRNA-LNP
Screening Material: hPTX-overexpressing 293FT
Challenges: No available purified protein & highly PTM
Results: Successfully generate 21 hPTX-positive parental clones

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
	Product	Cat.No.	Size	Maker	Qty	Data Sheet	MSDS
	mRNA 항원을 이용한 막단백질 항체 제작서비스	.	.	GENSCRIPT

제품 문의

제품명

이름*

직급*

소속/부서명*

교수님명*

이메일*

핸드폰 번호*

제목

내용

개인 정보 수집 및 이용 동의

mRNA 항원을 이용한 막단백질 항체 제작 서비스 | 고마바이오텍