특징
- HepaSH는 일본의 중앙실험동물연구소(CIEA)와 Biopredic사의 공동연구를 통해 개발된 신개념의 Human hepatocyte로, 인간 간세포를 humanized liver mice (TK-NOG mice)에 이식하여 얻은 인간 간세포 모델입니다.
- 동일한 조건의 인간 간세포를 지속적으로 공급할 수 있습니다.
- 장기 배양이 가능합니다. (monolayer culture에서 14일간 배양)
- 서로 다른 3명의 donor로 부터 파생된 hepatocyte를 이용함으로써, 분석결과의 신뢰성을 향상시킬 수 있습니다.
- Donor 1: Female, Caucasian, 12 years old
- Donor 2: Male, Caucasian, 3 months
- Donor 3: Male, African, 31 years old
- HepaSH 세포는 PHH와 유사한 수준의 약물 대사 효소 활성과 유전자 발현을 보입니다.
- Fresh suspended hepatocytes 형태로 제공되어 바로 plating하여 실험에 이용할 수 있습니다.
- PHH와 마찬가지로 약물동태 및 독성시험, 약리시험 등에 사용할 수 있습니다.
종류
| Cat. No. |
Product |
Donor 정보 |
세포 수 |
보관온도 |
| FSSH001 |
Fresh Suspended
Ready-to-plate HepaSH® |
Female,
Caucasian,12 years old |
5x10^6 cells/vial |
4℃ |
| FSSH002 |
Fresh Suspended
Ready-to-plate HepaSH® |
Male, Caucasian,3
months |
5x10^6 cells/vial |
4℃ |
| FSSH003 |
Fresh Suspended
Ready-to-plate HepaSH® |
Male, Caucasian,5
years old |
5x10^6 cells/vial |
4℃ |
Culture media
| Cat.
No. |
Product |
구성품 |
보관온도 |
| MIL130C |
OneStep PHep Thawing Medium for hepatocytes |
MIL130C×1 |
+4℃ |
| MIL221 |
Seeding Medium |
MIL600C×1, ADD221C×1 |
MIL600C：+4℃ |
| MIL222 |
Incubation Medium |
MIL600C×1, ADD222C×1 |
ADD221C, ADD222C：-20℃ |
| MIL600C |
Basal hepatic cell medium without additives |
MIL600C×1 |
+4℃ |
| ADD221C |
Additives for hepatocyte seeding medium |
ADD221C×1 |
-20℃ |
| ADD222C |
Additives for hepatocyte culture medium |
ADD222C×1 |
-20℃ |
참고 데이터
- 같은 donor 다른 mice 간의 drug-metabolizing enzyme activity 비교
- HepaSH Morphology and Life Style
- Metabolically Competent HH
참고 문헌
・M. Hasegawa, et al., “The reconstituted 'humanized liver' in TK-NOG mice is mature and functional.” Biochem Biophys Res Commun. 2011 Feb 18;405(3):405-10.
https://doi.org/10.1016/j.bbrc.2011.01.042
・H. Suemizu, et al., “Chimeric mice with humanized liver as a model for testing organophosphate and carbamate pesticide exposure.” Pest Manag Sci. 2018 Jun; 74(6): 1424–1430.
https://doi.org/10.1002/ps.4825
・S. Uehara, et al., “Expression and inducibility of cytochrome P450s inhuman hepatocytes isolated from chimeric mice with humanised livers.” Xenobiotica. 2019 Jun;49(6):678-687.
https://doi.org/10.1080/00498254.2018.1495346
・Yoshi Suemizu, "The usefulness of humanized liver mice in non-clinical research," Tanimoto School Toxicity Question Box. 2020, Vol. 22, pp. 32-40.
・P. Bachour-El Azzi, et al., “Expression and functional activity of cytochrome P450 enzymes in human hepatocytes with sustainable reproducibility for in vitro phenotyping studies.” Adv Pharmacol. 2022:95:285-305
https://doi.org/10.1016/bs.apha.2022.05.009
・Y. Uno, et al., “Cytochrome P450 1A2 and 2C enzymes autoinduced by omeprazole in dog hepatocytes and human HepaRG and HepaSH cells are involved in omeprazole 5-hydroxylation and sulfoxidation.” Xenobiotica. 2023 Dec;53(6-7):465-473.
https://doi.org/10.1080/00498254.2023.2266840
・S. Uehara, et al., “HepaSH cells: Experimental human hepatocytes with lesser inter-individual variation and more sustainable availability than primary human hepatocytes.” Biochem Biophys Res Commun. 2023 Jun 30:663:132-141.
https://doi.org/10.1016/j.bbrc.2023.04.054
・A. Zerdoug, et al., “Drug transporter expression and activity in cryopreserved humanhepatocytes isolated from chimeric TK-NOG mice with humanized livers.” Toxicol In Vitro. 2023 Aug:90:105592
https://doi.org/10.1016/j.tiv.2023.105592