SPINeasy® DNA/RNA Kit for Feces | 고마바이오텍

특징 

• 섬유질, 소화되지 않은 입자, 빌리루빈, 복합 다당류, 지질 등 여러 화합물이 섞여 있는 분변 샘플은 DNA 추출이 어렵거나 추출된 DNA의 품질이 저하됩니다. SPINeasy® DNA/RNA Kit for Feces는 독점적인 저해제 제거기술과 DNA/RNA에만 선택적으로 결합하는 신기술을 이용하여 성분에 상관없이 까다로운 분변 샘플로부터 microbiome genomic DNA와 RNA를 동시에 분리할 수 있습니다. 
• 분리된 DNA/RNA는 오염 물질을 함유하지 않으며, inhibitor 제거 과정이 필요없습니다.
• qPCR 및 RT-qPCR을 포함한 다운스트림 응용 분야에 즉시 사용할 수 있습니다. 

Figure 1. The SPINeasy® DNA/RNA Kit for Feces extracted DNA and RNA from various fecal samples with high yield and purity. Eleven fecal sample types were processed using SPINeasy® DNA/RNA Kit and competitor Q kit. Yield and purity ratios (A260/280 and A260/230) were measured by fluorometric quantification. Each dot on the plot represents a single extraction. Representative virtual gels (obtained using Agilent 4150 TapeStation analyses) were presented together with the DNA integrity (DIN) and RNA integrity (RIN) values. The red rectangle indicated the contamination of RNA samples with some high molecular weight DNA.

Unlike competitor Q kit, no cross-DNA contamination was observed in RNA samples extracted with the SPINeasy® DNA/RNA Kit for Feces. As shown in Agilent virtual gel (Figure 1, red rectangle) or agarose gel profile (Figure 2), some RNA samples extracted with the competitor Q kit contained DNA contamination; this highlights the outstanding performance of the SPINeasy® DNA/RNA Kit for Feces as compared to other similar commercial kit on the market.

Figure 2. No DNA contamination in the RNA samples extracted with the SPINeasy® DNA/RNA Kit for Feces. RNA samples extracted from horse, duck and pig fecal samples were visualized on agarose gel. Unlike competitor Q, no smeared nucleic acid corresponding to DNA contamination could be observed in RNA samples extracted with MP Biomedicals’ kit.