SPINeasy® DNA/RNA Kit for Soil | DNA와 RNA 동시추출 | 고마바이오텍

특징

• 하나의 토양 샘플에서 DNA와 RNA를 각각 추출할 수 있습니다.
• High biomass, high contiminant, 및 low biomass 샘플로부터 고효율로 고품질의 DNA를 추출할 수 있습니다.
• 재현성이 높은 결과를 얻을 수 있습니다.
• 간단한 과정을 통하여, DNA와 RNA를 동시 추출할 수 있습니다.
• 샘플의 양이 적을 때 효율적입니다.

구성품

사양

• Technology : Silica membrane technology
• Format : Mini Column
• Sample : Soil (e.g., sand/compost/sediments/topsoil)
• Sample amount : Up to 500 mg
• Observed yield : Up to 50 μg (DNA) / 30 μg (RNA) (sample-dependent)
• Elution volume : 30–100 μL
• Preparation time : <45 min (6 preps)

사용 방법

 데이터

그림 1. The SPINeasy® DNA/RNA Kit for Soil gives higher yield of nucleic acid than that of Competitor Z. Three types of soil samples (250 mg each) were used for DNA/RNA extraction. Yields were measured by fluorometric quantification. Each dot on the plot represents a single extraction.

그림 2. The SPINeasy® DNA/RNA Kit for Soil successfully extracted high-quality DNA and RNA from all challenging types of soil samples, including high biomass, high contaminant, and low biomass. Virtual gels obtained using the Agilent 4150 TapeStation analyses were shown along with the DNA integrity (DIN) and RNA integrity (RIN) values. Each dot on the plot represents a single sample.

그림 3. High purity and amplifiability of DNA/RNA extracted with SPINeasy® DNA/RNA Kit for Soil. (Purity) DNA/RNA purity was indicated by the Purity A260/A280 and A260/A230 ratios. (Humic Acid) Representative picture of the DNA and RNA outputs. A brownish coloration indicates the presence of humic acid contamination, in line with the quantification results using a spectrophotometer @ 400nm. (qPCR) The absence of inhibitor was further assessed using inhibitor-sensitive qPCR and RT-qPCR. One microliter of undiluted sample was amplified using primers targeting bacteria ribosomal 16s