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EverBrite Antifade Mounting Media 이미지

EverBrite Antifade Mounting Media

EverBrite Antifade Mounting Media는 면역형광 염색시 CF dye를 포함한 여러 형광 dye의 형광성을 유지시켜 줍니다. 특히 EverBrite TrueBlack® Hardset Mounting Medium은 Lipofuscin의 자가형광을 제거하여 백그라운드를 줄여줍니다.

프린트 제품 문의

EverBrite™ Mounting Medium 


  • Immunofluorescence (IF) imaging 시, CF dye를 포함한 형광 dye의 형광성을 유지시켜주는 antifade mounting medium 입니다. 
  • 형광물질의 photobleaching을 방지하기 위해 최적화되어 있습니다.
  • 굴절률은 1.46으로 coverslip glass와 immersion oil과 (1.5) 거의 동일합니다. 
  • 커버슬립을 CoverGripTM Coverslip Sealant로 밀봉후 이미징에 즉시 이용합니다. 
  • 슬라이드는 4도에서 1년 이상 보관할 수 있습니다. 
  • 핵 대조염색을 위한 DAPI를 포함하거나 포함하지 않고 사용할 수 있는 옵션이 있습니다.  
 EverBrite™ Mounting Medium
Cat. No.ProductCounterstain
23001EverBrite™ Mounting MediumNone
23002EverBrite™ Mounting Medium with DAPIDAPI

  

 

EverBrite™ 

Hardset Mounting Medium

  • CF dye를 포함한 형광 dye의 형광성을 유지시켜주는 antifade mounting medium로, 실온에서 24시간 이내에 완전히 경화됩니다.
  • Cy®3 및 Cy®5, Alexa Fluor® 647 및 DyLight® 649와 같은 cyanine 기반 형광과 호환됩니다.
  • 매니큐어나 sealant로 커버슬립 가장자리를 밀봉할 필요가 없습니다. 
  • 초기에 1.38의 굴절율을 가지며, 24시간 경화 후에는 1.42로 증가하고, 4일 후에는 1.46으로 증가하며 그 후에는 일정하게 유지됩니다. 


EverBrite TrueBlack® Hardset Mounting Medium-antifade
Unlike VECTASHIELD® Hardset, EverBrite™ Hardset does not quench the fluorescence of cyanine-based dyes. Methanol-fixed HeLa cells on coverslips were stained with mouse anti-tubulin antibody followed by Cy®5 goat anti-mouse secondary antibody. Coverslips were mounted using the indicated medium, cured according to the manufacturer's instructions, and then imaged by confocal microscopy. Other widely-used cyanine-based dyes include Cy®3, Alexa Fluor® 555, Alexa Fluor® 647, CF®555, CF®647, CF®660C, and CF®680. 

 

 

Cat. No.ProductCounterstain
23003EverBrite™ Hardset Mounting MediumNone
23004EverBrite™ Hardset Mounting Medium with DAPIDAPI
23016EverBrite™ Hardset with NucSpot® 640NucSpot® 640

 


EverBrite TrueBlack® Hardset Mounting Medium

  • 뇌, 망막 조직과 같은 노화된 동물 조직에서 Lipofuscin antofluorescene를 소멸시키는 antifade mounting media 입니다.
  • 적혈구나 세포외기질 등의 다른 소스의 백그라운드 감소에도 효과적입니다. 


EverBrite™ Hardset Mounting Medium
Methanol-fixed human cerebral cortex cryosections were stained with CF®488A Anti-GFAP, clone GA5+ASTRO/789 (glial cells, green), then mounted with EverBrite™ Hardset or EverBrite TrueBlack® Hardset mounting medium with NucSpot® 640 (nuclei, magenta). Lipofuscin autofluorescence was imaged in the Cy®3 channel (orange). Sections were imaged after curing overnight at room temperature. EverBriteTrueBlack® Hardset effectively quenched lipofuscin autofluorescence. 


EverBrite TrueBlack® Hardset Mounting Medium
Human cerebral cortex cryosections were stained with CF®405S GFAP Antibody (ASTRO/1974R) (glial cells, cyan), then mounted with EverBrite™ Hardset with NucSpot® 640, or EverBrite TrueBlack® Hardset with NucSpot® 640. Lipofuscin autofluorescence appears orange in the merged image and is effectively quenched after mounting in EverBrite TrueBlack®. Nuclei are stained with NucSpot® 640 (magenta).


Cat. No.ProductCounterstain
23017EverBrite TrueBlack® Hardset Mounting MediumNone
23018EverBrite TrueBlack® Hardset with DAPIDAPI
23019EverBrite TrueBlack® Hardset with NucSpot® 640NucSpot® 640

 


Drop-n-Stain EverBrite™ Mounting Medium   

 

  • CF dye 및 기타 여러 형광 dye의 보존을 위해 최적화된 봉입제 입니다.
  • Dropper bottle을 이용하여 피펫없이 분주할 수 있어 편리합니다. 

 

 Drop-n-Stain EverBrite™ Mounting Medium  

Cat. No.ProductCounterstain
23008Drop-n-Stain EverBrite™ Mounting MediumNone
23009Drop-n-Stain EverBrite™ Mounting Medium with DAPIDAPI

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
Drop-n-Stain EverBrite™ Mounting Medium 23008-T 2 ml BIOTIUM
Drop-n-Stain EverBrite™ Mounting Medium 23008 10 ml BIOTIUM
Drop-n-Stain EverBrite™ Mounting Medium with DAPI 23009 10 ml BIOTIUM
Drop-n-Stain EverBrite™ Mounting Medium with DAPI 23009-T 2 ml BIOTIUM
EverBrite TrueBlack® Hardset Mounting Medium 23017 1 ML BIOTIUM
EverBrite TrueBlack® Hardset Mounting Medium 23017-T 1 ML BIOTIUM
EverBrite TrueBlack® Hardset Mounting Medium with DAPI 23018-T 1 ML BIOTIUM
EverBrite TrueBlack® Hardset Mounting Medium with DAPI 23018 1 ML BIOTIUM
EverBrite TrueBlack® Hardset Mounting Medium with NucSpot® 640 23019 1 ML BIOTIUM
EverBrite TrueBlack® Hardset Mounting Medium with NucSpot® 640 23019-T 1 ML BIOTIUM
EverBrite™ Hardset Mounting Medium 23003 10 ml BIOTIUM
EverBrite™ Hardset Mounting Medium with DAPI 23004 10 ml BIOTIUM
EverBrite™ Hardset Mounting Medium with NucSpot® 640 23016-T 1 ML BIOTIUM
EverBrite™ Hardset Mounting Medium with NucSpot® 640 23016 1 ML BIOTIUM
EverBrite™ Mounting Medium 23001 10 ml BIOTIUM
EverBrite™ Mounting Medium with DAPI 23002 10 ml BIOTIUM
L-Ascorbic acid (50 mg) 4055 50 mg TOCRIS
Mounting Medium, Immuno-Fluore™ 08622701 25 ml MPBIO
Trolox (100 mg) 6002 100 mg TOCRIS
전체보기
Maker
BIOTIUM
Cat.No.
23008-T
Product
Drop-n-Stain EverBrite™ Mounting Medium
Size
2 ml
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
23008
Product
Drop-n-Stain EverBrite™ Mounting Medium
Size
10 ml
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
23009
Product
Drop-n-Stain EverBrite™ Mounting Medium with DAPI
Size
10 ml
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
23009-T
Product
Drop-n-Stain EverBrite™ Mounting Medium with DAPI
Size
2 ml
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
23017
Product
EverBrite TrueBlack® Hardset Mounting Medium
Size
1 ML
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
23017-T
Product
EverBrite TrueBlack® Hardset Mounting Medium
Size
1 ML
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
23018-T
Product
EverBrite TrueBlack® Hardset Mounting Medium with DAPI
Size
1 ML
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
23018
Product
EverBrite TrueBlack® Hardset Mounting Medium with DAPI
Size
1 ML
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
23019
Product
EverBrite TrueBlack® Hardset Mounting Medium with NucSpot® 640
Size
1 ML
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
23019-T
Product
EverBrite TrueBlack® Hardset Mounting Medium with NucSpot® 640
Size
1 ML
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
23003
Product
EverBrite™ Hardset Mounting Medium
Size
10 ml
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
23004
Product
EverBrite™ Hardset Mounting Medium with DAPI
Size
10 ml
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
23016-T
Product
EverBrite™ Hardset Mounting Medium with NucSpot® 640
Size
1 ML
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
23016
Product
EverBrite™ Hardset Mounting Medium with NucSpot® 640
Size
1 ML
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
23001
Product
EverBrite™ Mounting Medium
Size
10 ml
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
23002
Product
EverBrite™ Mounting Medium with DAPI
Size
10 ml
Qty
Data Sheet
MSDS
Maker
TOCRIS
Cat.No.
4055
Product
L-Ascorbic acid (50 mg)
Size
50 mg
Qty
Data Sheet
MSDS
Maker
MPBIO
Cat.No.
08622701
Product
Mounting Medium, Immuno-Fluore™
Size
25 ml
Qty
Maker
TOCRIS
Cat.No.
6002
Product
Trolox (100 mg)
Size
100 mg
Qty
Data Sheet
MSDS
전체보기

추천제품

관련제품

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
CoverGrip™ Coverslip Sealant 23005 15 ml BIOTIUM
CoverGrip™ Coverslip Sealant Refill 23005-1 100 ml BIOTIUM
DAPI 40011 10 mg BIOTIUM
DAPI, 10mg/mL in H2O 40043 1 ml BIOTIUM
DAPI, dilactate (4’,6-diamidino-2-phenylindole, dilactate) 40009 10 mg BIOTIUM

자료

  • Histology, Hematology and Cytology

    Histology, Hematology and Cytology 이미지

    [MPBIO]

    조직세포염색에 이용되는 시약에 대한 안내입니다.

웨비나/Video

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제품 문의

 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
Maker
Cat.No.
Product
Size
Qty
Data Sheet
MSDS

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