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제품 상세
Epigenetics Assay Kit | Methylation 및 HDAC 분석 이미지

Epigenetics Assay Kit | Methylation 및 HDAC 분석

HDAC Assay Kit와 Global Methylation ELISA Kit를 소개합니다.

프린트 제품 문의
주요 제품


HDAC Assay Kit (#GR107011)

  • Histone deacetylases (HDACs)의 활성을 분석합니다. 
  • 컬러 방법으로 측정합니다.
  • Cell-permeable한 substrate를 통해 세포 lysis 필요 없이 cell culture에 바로 이용할 수 있습니다. 
  • 구성 : 
    • Assay buffer 12 mL
    • Developer 12 mL
    • Substrate 100 uL
    • HDAC inhibitor(TSA) 10 uL 1mM

HDAC Assay Kit




Global Methylation ELISA Kit (#ARG82223)

 

  • 암세포에서 일어나는 DNA modification 결과인 5'-methyl-2'-deoxycytidine (5MedCyd)를 측정하는 Kit 입니다.
  • 세포 혹은 조직에서 얻은 genomic DNA나 Urine 샘플에서 바로 5’-methyl-2’-deoxycytidine (5MedCyd)를 경쟁적 효소 면역측정 방법으로 측정합니다.
  • 5MedCyd DNA가 미리 코팅된 EIA plate가 구성품으로 제공됩니다.
  • Detection sensitivity range: 30 nM - 4 uM
  • 구성 : 
    • DNA High-Binding Plate 
    • Anti-5MedCyd Antibody
    • Secondary Antibody, HRP Conjugate 
    • Assay Diluent 
    • 10X Wash Buffer 
    • Substrate Solution 
    • Stop Solution 
    • 5MedCyd Standard 
    • 5MedCyd DNA Conjugate 

      

     Global Methylation ELISA Kit 

      


    사용 논문

     

    STA-380
    STA-380-5    		 　
      1. Mungala Lengo, A. et al. (2020). Relationship between redox potential of glutathione and DNA methylation level in liver of newborn guinea pigs. Epigenetics. doi: 10.1080/15592294.2020.1781024.
      2. Holubek, R. et al. (2020). The Recovery of Soybean Plants after Short-Term Cadmium Stress. Plants (Basel). 9(6):E782. doi: 10.3390/plants9060782.
      3. Quintanilla-Mena, M. et al. (2019). Biological responses of shoal flounder (Syacium gunteri) to toxic environmental pollutants from the southern Gulf of Mexico. Environ Pollut. doi: 10.1016/j.envpol.2019.113669.
      4. Pennarossa, G. et al. (2019). Rho Signaling-Directed YAP/TAZ Regulation Encourages 3D Spheroid Colony Formation and Boosts Plasticity of Parthenogenetic Stem Cells. Adv Exp Med Biol. doi: 10.1007/5584_2019_423.
      5. Onishi, A. et al. (2019). Urine 5MedC, a Marker of DNA Methylation, in the Progression of Chronic Kidney Disease. Disease Markers. doi: 10.1155/2019/5432453.
      6. Jarmasz, J.S. et al (2019). DNA methylation and histone post-translational modification stability in post-mortem brain tissue. Clin Epigenetics. 11(1):5. doi: 10.1186/s13148-018-0596-7.
      7. Pennarossa, G. et al. (2019). Use of a PTFE Micro-Bioreactor to Promote 3D Cell Rearrangement and Maintain High Plasticity in Epigenetically Erased Fibroblasts. Stem Cell Rev. 15(1):82-92. doi: 10.1007/s12015-018-9862-5.
      8. Pennarossa, G. et al. (2018). Epigenetic Erasing and Pancreatic Differentiation of Dermal Fibroblasts into Insulin-Producing Cells are Boosted by the Use of Low-Stiffness Substrate. Stem Cell Rev. 14(3):398-411. doi: 10.1007/s12015-017-9799-0.
      9. Shah, S.M. et al. (2017). Retinoic acid induces differentiation of buffalo (Bubalus bubalis) embryonic stem cells into germ cells. Gene. pii: S0378-1119(17)30382-7. doi: 10.1016/j.gene.2017.05.037.
      10. Manzoni, E.F.M. et al. (2016). 5-azacytidine affects TET2 and histone transcription and reshapes morphology of human skin fibroblasts. Sci. Rep. 6:37017.
      11. Lynch, E. W. et al. (2016). Cyclical DNA methyltransferase 3a expression is a seasonal and oestrus timer in reproductive tissues. Endocrinology. doi:10.1210/en.2015-1988.
      12. Shah, S. M. et al. (2016). Testicular cell conditioned medium supports embryonic stem cell differentiation towards germ lineage and to spermatocyte-and oocyte-like cells. Theriogenology. doi:10.1016/j.theriogenology.2016.02.025.
      13. Kurdyukov, S. & Bullock, M. (2016). DNA methylation analysis: Choosing the right method. Biology.doi:10.3390/biology5010003.
      14. Shah, S. M. et al. (2015). Bone morphogenetic protein 4 (BMP4) induces buffalo (Bubalus bubalis) embryonic stem cell differentiation into germ cells. Biochimie. 119:113-124.
      15. Creppy, E. E. et al. (2014). Study of epigenetic properties of Poly(HexaMethylene Biguanide) hydrochloride (PHMB). Int J Environ Res Public Health. 11:8069-8092.
      16. Jefferson, W. et al. (2013). Persistently Altered Epigenetic Marks in the Mouse Uterus After Neonatal Estrogen Exposure. Mol Endocrinol. 27:1666-1677.

     

    주문정보

    주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
      Product Cat.No. Size Maker Qty Data Sheet MSDS
    		Genorise® HDAC Fluorometric Assay Kit GR107011 100 tests GENORISE
    		Global DNA Methylation ELISA Kit ARG82223 96 wells ARIGO
    		Global DNA Methylation ELISA Kit (5’-methyl-2’-deoxycytidine Quantitation) STA-380 96 assays CELL BIOLABS
    		Global DNA Methylation ELISA Kit (5’-methyl-2’-deoxycytidine Quantitation) STA-380-5 5x96 assays CELL BIOLABS
    		Human Asymmetrical Dimethylarginine (ADMA) ELISA Kit, 96 tests 100-310-ADM 1 kit ALPHA DIAGNOSTICS
    		Human Symmetrical Dimethylarginine (SDMA) ELISA Kit, 96 tests 100-340-SDM 1 kit ALPHA DIAGNOSTICS
    Maker
    GENORISE
    Cat.No.
    GR107011
    Product
    Genorise® HDAC Fluorometric Assay Kit
    Size
    100 tests
    Qty
    Maker
    ARIGO
    Cat.No.
    ARG82223
    Product
    Global DNA Methylation ELISA Kit
    Size
    96 wells
    Qty
    Data Sheet
    Maker
    CELL BIOLABS
    Cat.No.
    STA-380
    Product
    Global DNA Methylation ELISA Kit (5’-methyl-2’-deoxycytidine Quantitation)
    Size
    96 assays
    Qty
    Data Sheet
    MSDS
    Maker
    CELL BIOLABS
    Cat.No.
    STA-380-5
    Product
    Global DNA Methylation ELISA Kit (5’-methyl-2’-deoxycytidine Quantitation)
    Size
    5x96 assays
    Qty
    Data Sheet
    MSDS
    Maker
    ALPHA DIAGNOSTICS
    Cat.No.
    100-310-ADM
    Product
    Human Asymmetrical Dimethylarginine (ADMA) ELISA Kit, 96 tests
    Size
    1 kit
    Qty
    Data Sheet
    MSDS
    Maker
    ALPHA DIAGNOSTICS
    Cat.No.
    100-340-SDM
    Product
    Human Symmetrical Dimethylarginine (SDMA) ELISA Kit, 96 tests
    Size
    1 kit
    Qty
    MSDS

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      Product Cat.No. Size Maker Qty Data Sheet MSDS

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