| Nuclear
Stains |
Cat. No. |
Color (Ex/Em*) |
Cell
permeability |
For live/fixed
cells |
Fix after
staining? |
Features |
EverBrite™ Mounting
Medium w/DAPI
EverBrite™ Hardset w/DAPI
Drop-n-Stain EverBrite™ w/DAPI |
23002
23004
23009 |
Blue (358/461 nm) |
N/A |
Mounting fixed samples |
N/A |
• Hardset and wet-set mounting
media
• Broad dye compatibility
• Available with DAPI for one-step mounting and staining
• Drop-n-Stain EverBrite™ comes in convenient dropper bottle |
DAPI
DAPI, 10 mg/mL in H2O
DAPI, dilactate 10 mg in H2O |
40011
40043
40009 |
Blue (358/461 nm) |
Membrane permeant |
Live cells
Fixed cells |
Yes |
• Classic nuclear counterstain for
fixed cells
• Can be used at higher concentrations to stain live cells
• Dilactate salt has improved water solubility |
Hoechst 33258, 10 mg/mL in H2O
Hoechst 33258, pentahydrate
Hoechst 33342, 10 mg/mL in H2O
Hoechst 33342, trihydrochloride trihydrate |
40044
40045
40046
40047 |
Blue (358/461 nm) |
Membrane permeant |
Live cells
Fixed cells |
Yes |
• Classic nuclear counterstain for
live cells
• Can also be used on fixed cells |
| Oxazole Gold (SYBR®
Gold) |
40094 |
Green (496/539) |
Membrane permeant |
Live cells |
No 2 |
• Aka SYBR® Gold, an
ultrasensitive DNA and RNA gel stain
• Can be used for live cell staining of nuclei and mitochondrial DNA |
| NucSpot® 470 Nuclear
Stain |
40083 |
Green (460/546 nm) |
Membrane impermeant |
Dead cells
Fixed cells |
No 1 |
• Nuclear-specific green
counterstain for fixed cells
• Selectively stains dead cells in live cultures
• Excellent match for blue LED excitation sources |
| Thiazole Green (SYBR®
Green I) |
40086 |
Green (498/522 nm) |
Membrane permeant |
Live cells
Fixed cells |
No 2 |
• Aka SYBR® Green I, a well known
DNA gel stain and qPCR dye
• Can be used as a green nuclear stain for all cells in live cultures
• Loses nuclear specificity after fixation
• Can be excited by 488 nm laser line |
| NucSpot® Live 488
Nuclear Stain |
40081 |
Green (500/515 nm) |
Membrane permeant |
Live cells
Fixed cells |
Yes |
• Low-toxicity nuclear stain
• Fix before or after labeling
• Live cell staining may require verapamil (included) |
| Live-or-Dye NucFix™
Red |
32010 |
Red (520/610 nm) |
Membrane impermeant |
Dead cells |
Yes |
• Reactive nuclear stain for dead
cells
• Specifically stains dead cell nuclei
• Fix/permeabilize without dye transfer between cells |
| NucSpot® Live 650
Nuclear Stain |
40082 |
Far-red (650/675 nm) |
Membrane permeant |
Live cells
Fixed cells |
Yes |
• Low-toxicity nuclear stain for
the Cy®5 channel
• Fix before or after labeling
• Live cell staining may require verapamil (included)
• Compatible with SIM, STED, or STORM |
| RedDot™1 Far-Red
Nuclear Stain |
40060 |
Far-red (662/694 nm) |
Membrane permeant |
Live cells |
No 2 |
• For short-term live cell
staining (≤4 hours)
• Analyze DNA content/cell cycle by flow cytometry
• Useful for cell number normalization for In Cell Western®
• Can be excited at wavelengths between 488 and 647 nm
• Detect in Cy®5 or APC channel |
| RedDot™2 Far-Red
Nuclear Stain |
40061 |
Far-red (665/695 nm) |
Membrane impermeant |
Dead cells
Fixed cells |
No 1 |
• Far-red nuclear stain for dead
or fixed cells
• Selectively stains dead cells
• Specific nuclear counterstain for fixed cells
• Can be excited at wavelengths between 488 and 647 nm
• Detect in Cy®5 or APC channel |