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제품 상세
핵 대조 염색을 위한 NucSpot stain 이미지

핵 대조 염색을 위한 NucSpot stain

NucSpot® Nuclear Stain은 녹색에서 근적외선까지 다양한 색상으로 제공되는 핵 대조 염색액 입니다. NucSpot® Live Cell Nuclear Stain은 Live cell 이미징에 사용될 수 있습니다.

NucSpot® Nuclear Stains

 

특징


  • NucSpot은 DAPI와 달리 녹색에서 근적외선까지 다양한 색상으로 핵을 염색할 수 있는 핵 대조 염색액 입니다.
  • DNA에 결합하기 전까지는 최소한의 형광을 띠고, no-wash 방법으로 핵을 염색할 수 있습니다. 
  • 핵과 세포질을 모두 염색하는 기존의 다른 핵산 dye와는 달리, 핵에만 특이적으로 결합합니다. 
  • 유세포 분석이나 형광 이미징 실험시, 고정되지 않은 세포에서 dead cell만 선택적으로 염색할 수 있습니다. 
  • NucSpot® 470 및 NucSpot® Far-Red는 DNA 함량분석에 사용될 수 있습니다. 

종류

Product Ex/Em Detection Channel Size Cat. No.
NucSpot® 500/515 497/513 nm FITC* 20 uL 41040-T
100 uL 41040
NucSpot® 555/570 559/566 nm Cy®3 or PE* 20 uL 41033-T
100 uL 41033
NucSpot® 568/580 572/583 nm Cy®3 or PE* 20 uL 41036-T
100 uL 41036
NucSpot® 594/615 603/613 nm Texas Red® or PE-Texas Red®* 20 uL 41037-T
100 uL 41037
NucSpot® 650/665 653/671 nm Cy®5 or APC* 20 uL 41034-T
100 uL 41034
NucSpot® 680/700 683/707 nm Cy®5.5* 20 uL 41035-T
100 uL 41035
NucSpot® 750/780 757/780 nm Cy®7 or APC-Cy®7* 20 uL 41038-T
100 uL 41038
* May show crosstalk in lower wavelength detection channels. Perform single-stain controls before combining with other probes.
† NucSpot® Far-Red is designed for flow cytometry optical systems and is not recommended for fluorescence microscopy.


PFA-fixed, Triton® X-100 permeabilized HeLa cells stained with NucSpot® Nuclear Stains in PBS and imaged by confocal microscopy without a wash step. All stains were used at 1X concentration except for NucSpot® 750/780, which was used at 5X concentration in order to image using 640 nm excitation.



Live dead discrimination by flow cytometry using NucSpot® Nuclear Stains. A mixture of untreated and heat-killed Jurkat cells was stained with each NucSpot® Nuclear Stain and analyzed by flow cytometry on a BD CytoFLEX flow cytometerin the detection channel shown on the histogram x-axis. Solid peaks represent stained cells; open gray peaks represent unstained cells. The concentrations of each dye used were: 1X NucSpot® 470, 0.1X NucSpot® 500/515, 0.5X NucSpot® 555/565, 1X NucSpot® 568/580; 0.5X NucSpot® 594/615, 0.05X NucSpot® 650/665, 0.2X NucSpot® 680/700, 0.5X NucSpot® 750/780.



NucSpot® Live Cell Nuclear Stains 

 

특징


  • NucSpot® Live Nuclear Stain은 살아있는 세포의 실시간 이미징에 이용할 수 있는 저독성 핵 염색액 입니다.
  • 세척이 필요없으며, 염색은 최대 72시간 동안 안정적 입니다. 
  • NucSpot® Live 488은 FITC 녹색 형광(Ex/Em 500/515 nm)을, NucSpot® Live 650은 Cy®5 채널에서 검출하기 위한 원적외선 형광(650/675 nm)을 가지며, SIM 및 STED의 초고해상도 이미징에도 사용될 수 있습니다. 

종류

Product Ex/Em Detection Channel Size Cat. No.
NucSpot® Live 488 500/515 nm FITC 10 uL 40081-T
50 uL 40081
NucSpot® Live 650 650/675 nm Cy®5 10 uL 40082-T
50 uL 40082

 


 

 


 

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
NucSpot® 470, 1000X in DMSO 40083 200 ul BIOTIUM
NucSpot® 470, 1000X in DMSO 40083-T 20 ul BIOTIUM
NucSpot® 500/515, 1000X in DMSO 41040 100UL BIOTIUM
NucSpot® 500/515, 1000X in DMSO (trial size) 41040-T 20UL BIOTIUM
NucSpot® 555/570, 1000X in DMSO 41033 100ul BIOTIUM
NucSpot® 555/570, 1000X in DMSO (trial size) 41033-T 20ul BIOTIUM
NucSpot® 568/580, 1000X in DMSO 41036 100ul BIOTIUM
NucSpot® 568/580, 1000X in DMSO (trial size) 41036-T 20ul BIOTIUM
NucSpot® 594/615, 1000X in DMSO 41037 100ul BIOTIUM
NucSpot® 594/615, 1000X in DMSO (trial size) 41037-T 20ul BIOTIUM
NucSpot® 650/665, 1000X in DMSO 41034 100ul BIOTIUM
NucSpot® 650/665, 1000X in DMSO (trial size) 41034-T 20ul BIOTIUM
NucSpot® 680/700, 1000X in DMSO 41035 100ul BIOTIUM
NucSpot® 680/700, 1000X in DMSO (trial size) 41035-T 20ul BIOTIUM
NucSpot® 750/780, 1000X in DMSO 41038 100ul BIOTIUM
NucSpot® 750/780, 1000X in DMSO (trial size) 41038-T 20ul BIOTIUM
NucSpot® Far-Red, 1000X in DMSO 40085-T 50 ul BIOTIUM
NucSpot® Far-Red, 1000X in DMSO 40085 0.5 ml BIOTIUM
NucSpot® Live 488, 1000X in DMSO 40081 50 ul BIOTIUM
NucSpot® Live 488, 1000X in DMSO 40081-T 10 ul BIOTIUM
NucSpot® Live 650, 1000X in DMSO 40082 50 ul BIOTIUM
NucSpot® Live 650, 1000X in DMSO 40082-T 10 ul BIOTIUM
전체보기
Maker
BIOTIUM
Cat.No.
40083
Product
NucSpot® 470, 1000X in DMSO
Size
200 ul
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
40083-T
Product
NucSpot® 470, 1000X in DMSO
Size
20 ul
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
41040
Product
NucSpot® 500/515, 1000X in DMSO
Size
100UL
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
41040-T
Product
NucSpot® 500/515, 1000X in DMSO (trial size)
Size
20UL
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
41033
Product
NucSpot® 555/570, 1000X in DMSO
Size
100ul
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
41033-T
Product
NucSpot® 555/570, 1000X in DMSO (trial size)
Size
20ul
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
41036
Product
NucSpot® 568/580, 1000X in DMSO
Size
100ul
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
41036-T
Product
NucSpot® 568/580, 1000X in DMSO (trial size)
Size
20ul
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
41037
Product
NucSpot® 594/615, 1000X in DMSO
Size
100ul
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
41037-T
Product
NucSpot® 594/615, 1000X in DMSO (trial size)
Size
20ul
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
41034
Product
NucSpot® 650/665, 1000X in DMSO
Size
100ul
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
41034-T
Product
NucSpot® 650/665, 1000X in DMSO (trial size)
Size
20ul
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
41035
Product
NucSpot® 680/700, 1000X in DMSO
Size
100ul
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
41035-T
Product
NucSpot® 680/700, 1000X in DMSO (trial size)
Size
20ul
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
41038
Product
NucSpot® 750/780, 1000X in DMSO
Size
100ul
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
41038-T
Product
NucSpot® 750/780, 1000X in DMSO (trial size)
Size
20ul
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
40085-T
Product
NucSpot® Far-Red, 1000X in DMSO
Size
50 ul
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
40085
Product
NucSpot® Far-Red, 1000X in DMSO
Size
0.5 ml
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
40081
Product
NucSpot® Live 488, 1000X in DMSO
Size
50 ul
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
40081-T
Product
NucSpot® Live 488, 1000X in DMSO
Size
10 ul
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
40082
Product
NucSpot® Live 650, 1000X in DMSO
Size
50 ul
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
40082-T
Product
NucSpot® Live 650, 1000X in DMSO
Size
10 ul
Qty
Data Sheet
MSDS
전체보기

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MSDS

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