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제품 상세

Antibody

6.5 - 270 kDa 범위를 커버하는 3가지 컬러 밴드를 포함하며,
최대 100%까지의 transfer 효과를 개런티하는 Prestained protein ladder를 소개합니다.

배경

 

RFP는 빨간색-주황색의 형광을 내는 형광단으로 처음에는 DsRed라는 이름으로 불리웠습니다. λex 최대값은 558nm, λem 최대값은 583nm입니다. mCherry는 DsRed에서 파생된 빨간색 형광단백질이며, 세포 내 구성요소에 표지를 할 때 사용됩니다. 



 

특징

  

  • 세포나 조직에서 단백질 위치를 감지하는 데에 이용되는 RFP (Red Fluorescent Protein), mCherry, tdTomato 등 red 형광 단백질에 대한 goat 항체입니다. 
  • 일부 다른 red 형광 단백질에 교차반응이 있지만, GFP에는 교차반응이 없습니다.
  • Rabbit, mouse antibody와 함께 triple co-staining에 이용할 수 있습니다. 
  • ICC, IHC 등의 immunostaining 및 WB에 사용 가능합니다.

 

 

종류


anti-RFP antibody (#ARG55744)    
Product Description Goat Polyclonal antibody recognizes RFP  
Tested Reactivity Other  
Tested Application ICC/IF, IHC-Fr, IHC-P, WB  
Specificity This antibody recognizes red fluorescent protein (dsRed), mCherry, mOrange, mPlum, mRFP, mStrawberry and tdTomato. It does not cross-react to GFP.  
Host Goat  
Clonality Polyclonal  
Isotype IgG  
Target Name RFP  
Immunogen Purified recombinant RFP peptide produced in E. coli  
Conjugation Un-conjugated  
Alternate Names Cherry fluorescent protein; dsRed; red fluorescent protein; tdTomato  
         
  anti-tdTomato antibody (#ARG55724)    
Product Description Goat Polyclonal antibody recognizes tdTomato  
Tested Reactivity Other  
Tested Application ICC/IF, IHC-Fr, IHC-P, WB  
Specificity This antibody recognizes tdTomato, mCherry and red fluorescent protein (DsRed). It does not cross-react to GFP.  
Host Goat  
Clonality Polyclonal  
Isotype IgG  
Target Name tdTomato  
Immunogen Purified full-length recombinant protein produced in E. coli.  
Conjugation Un-conjugated  
Alternate Names Cherry fluorescent protein; DsRed; mCherry; red fluorescent protein; RFP  
         
  anti-mCherry antibody (#ARG55723)    
Product Description Goat Polyclonal antibody recognizes mCherry  
Tested Reactivity Other  
Tested Application ICC/IF, IHC-Fr, IHC-P, WB  
Specificity This antibody recognizes mCherry, red fluorescent protein (dsRed) and tdTomato. It does not cross-react to GFP.  
Host Goat  
Clonality Polyclonal  
Isotype IgG  
Target Name mCherry  
Immunogen Purified recombinant peptide produced in E. coli  
Conjugation Un-conjugated  
Alternate Names Cherry fluorescent protein; dsRed; red fluorescent protein; tdTomato  
         




실험 결과

 

 

ARG55723 anti-mCherry antibody WB image. Western blot: 100 µg of 293HEK cells transduced with myc-mCherry Ad and stained with ARG55723 anti-mCherry antibody at 1:1000 dilution.

 


 

ARG55723 anti-mCherry antibody ICC/IF image. Immunocytochemistry: 293HEK cells transfected with mCherry-Rab1a and stained with ARG55723 anti-mCherry antibody at 1:50 dilution. Cells were fixed with 4% of PFA. 

 

 ARG55724 anti-tdTomato antibody WB image. Western blot: 50 µg of 293HEK cells transduced with cDNA and stained with ARG55724 anti-tdTomato antibody at 1:1000 dilution.

 


ARG55724 anti-tdTomato antibody ICC/IF image. Immunocytochemistry: 293HEK cells transfected with cDNA and stained with ARG55724 anti-tdTomato antibody at 1:50 dilution. Cells were fixed with 4% of PFA. 

 

 

ARG55744 anti-RFP antibody WB image Western blot. 50 µg of 293HEK cells transduced with RFP Ad stained with ARG55744 anti-RFP antibody at 1:2000 dilution. 

 

 

 ARG55744 anti-RFP antibody ICC/IF image. Immunocytochemistry: 293HEK cells transfected with RFP stained with ARG55744 anti-RFP antibody at 1:50 dilution. Cells were fixed with 4% PFA.

 

 

 

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
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자료

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제품 문의

0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
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