최적의 유전자 Cloning을 위한 Vector Selection Guide | 고마바이오텍

Vector Selection Guide 

 

Vector Name and Map	Host	Promoter	Bacterial Antibiotics	Copy number	N-terminal Epitope tag	Cleavage site	C-terminal Epitope tag
pGenLenti	Mammalian	T7, CMV	Ampicillin	High			C-DYKDDDDK
pcDNA3.1(+)	Mammalian	CMV	Ampicillin	High
pcDNA3.1(-)	Mammalian	CMV	Ampicillin	High
pcDNA3.1(+)-myc-His A	Mammalian	CMV	Ampicillin	High			C-Myc, C-His
pcDNA3.1(+)-myc-His B	Mammalian	CMV	Ampicillin	High			C-Myc, C-His
pcDNA3.1(+)-myc-His C	Mammalian	CMV	Ampicillin	High			C-Myc, C-His
pcDNA3.1(-)-myc-His A	Mammalian	CMV	Ampicillin	High			C-Myc, C-His
pcDNA3.1(-)-myc-His B	Mammalian	CMV	Ampicillin	High			C-Myc, C-His
pcDNA3.1(-)-myc-His C	Mammalian	CMV	Ampicillin	High			C-Myc, C-His
pCI-Neo	Mammalian	CMV	Ampicillin	High
pcDNA3.1(+)-C-DYK	Mammalian	CMV	Ampicillin	High			C-DYKDDDDK
pcDNA3.1(+)-C-HA	Mammalian	CMV	Ampicillin	High			C-HA
pcDNA3.1(+)-C-6His	Mammalian	CMV	Ampicillin	High			C-His
pcDNA3.1(+)-C-Myc	Mammalian	CMV	Ampicillin	High			C-MYC
pcDNA3.1(+)-N-DYK	Mammalian	CMV	Ampicillin	High	N-DYKDDDDK
pcDNA3.1(+)-N-HA	Mammalian	CMV	Ampicillin	High	N-HA
pcDNA3.1(+)-N-6His	Mammalian	CMV	Ampicillin	High	N-His
pcDNA3.1(+)-N-Myc	Mammalian	CMV	Ampicillin	High	N-Myc
pcDNA3.1(+)-N-GST(Thrombin)	Mammalian	CMV	Ampicillin	High	N-GST(Thrombin)	Thrombin
pcDNA3.1(+)-N-GST(TEV)	Mammalian	CMV	Ampicillin	High	N-GST(TEV)	TEF
pcDNA3.1/Hygro(+)	Mammalian	CMV	Ampicillin	High
pcDNA3.1/Hygro(-)	Mammalian	CMV	Ampicillin	High
pcDNA3.1/Zeo (+)	Mammalian	CMV	Ampicillin	High
pcDNA3.1/Zeo (-)	Mammalian	CMV	Ampicillin	High
pCMV-3Tag-1a	Mammalian	CMV	Kanamycin	High	N-3*DYKDDDDK
pCMV-3Tag-2a	Mammalian	CMV	Kanamycin	High	N-3*Myc
pCMV-3Tag-3a	Mammalian	CMV	Kanamycin	High			C-3*DYKDDDDK
pCMV-3Tag-4a	Mammalian	CMV	Kanamycin	High			C-3*Myc
pcDNA3.1(+)-C-eGFP	Mammalian	CMV	Ampicillin	High			C-eGFP
pcDNA3.1(+)-N-eGFP	Mammalian	CMV	Ampicillin	High	N-eGFP
pcDNA3.1(+)-P2A-eGFP	Mammalian	CMV	Ampicillin	High			C-eGFP
pcDNA3.1(+)-P2A	Mammalian	CMV	Ampicillin	High
pcDNA3.1(+)-N-DYK-P2A	Mammalian	CMV	Ampicillin	High			C-DYKDDDDK
pcDNA3.1(+)-C-DYK-P2A	Mammalian	CMV	Ampicillin	High			C-DYKDDDDK
pCMV-3Tag-1a-P2A	Mammalian	CMV	Kanamycin	High	N-3*DYKDDDDK
pCMV-3Tag-3a-P2A	Mammalian	CMV	Kanamycin	High			C-3*DYKDDDDK
pcDNA3.4	Mammalian	CMV	Ampicillin	High
pBluescript II KS(-)	Bacterial	T7	Ampicillin	High
pBluescript II KS(+)	Bacterial	T7	Ampicillin	High
pBluescript II SK(-)	Bacterial	T7	Ampicillin	High
pBluescript II SK(+)	Bacterial	T7	Ampicillin	High
pET-3a	Bacterial	T7	Ampicillin	Low	N-T7
pET-3b	Bacterial	T7	Ampicillin	Low	N-T7
pET-3c	Bacterial	T7	Ampicillin	Low	N-T7
pET-3d	Bacterial	T7	Ampicillin	Low	N-T7
pET-9a	Bacterial	T7	Kanamycin	Low	N-T7
pET-11a	Bacterial	T7 & Lac	Ampicillin	Low	N-T7
pET-11b	Bacterial	T7 & Lac	Ampicillin	Low	N-T7
pET-11c	Bacterial	T7 & Lac	Ampicillin	Low	N-T7
pET-11d	Bacterial	T7 & Lac	Ampicillin	Low	N-T7
pET-14b	Bacterial	T7	Ampicillin	Low	N-His
pET-15b	Bacterial	T7 & Lac	Ampicillin	Low	N-His
pET-16b	Bacterial	T7 & Lac	Ampicillin	Low	N-His
pET-17b	Bacterial	T7	Ampicillin	Low	N-T7
pET-19b	Bacterial	T7 & Lac	Ampicillin	Low	N-His
pET-20b(+)	Bacterial	T7	Ampicillin	Low	N-pelB		C-His
pET-21a(+)	Bacterial	T7 & Lac	Ampicillin	Low	N-T7		C-His
pET-21b(+)	Bacterial	T7 & Lac	Ampicillin	Low	N-T7		C-His
pET-21d(+)	Bacterial	T7 & Lac	Ampicillin	Low	N-T7		C-His
pET-22b(+)	Bacterial	T7 & Lac	Ampicillin	Low	N-pelB		C-His
pET-23a(+)	Bacterial	T7	Ampicillin	Low	N-T7		C-His
pET-24a(+)	Bacterial	T7 & Lac	Kanamycin	Low	N-T7		C-His
pET-24b(+)	Bacterial	T7 & Lac	Kanamycin	Low	N-T7		C-His
pET-24c(+)	Bacterial	T7 & Lac	Kanamycin	Low	N-T7		C-His
pET-24d(+)	Bacterial	T7 & Lac	Kanamycin	Low	N-T7		C-His
pET-25b(+)	Bacterial	T7 & Lac	Ampicillin	Low	N-pelB		C-HSV, C-His
pET-26b(+)	Bacterial	T7 & Lac	Kanamycin	Low	N-pelB		C-His
pET-27b(+)	Bacterial	T7 & Lac	Kanamycin	Low	N-pelB		C-HSV, C-His
pET-28a(+)	Bacterial	T7 & Lac	Kanamycin	Low	N-His, N-T7		C-His
pET-28b(+)	Bacterial	T7 & Lac	Kanamycin	Low	N-His, N-T8		C-His
pET-28c(+)	Bacterial	T7 & Lac	Kanamycin	Low	N-His, N-T9		C-His
pET-29a(+)	Bacterial	T7 & Lac	Kanamycin	Low	N-S-tag		C-His
pET-29b(+)	Bacterial	T7 & Lac	Kanamycin	Low	N-S-tag		C-His
pET-29c(+)	Bacterial	T7 & Lac	Kanamycin	Low	N-S-tag		C-His
PET-30a(+)	Bacterial	T7 & Lac	Kanamycin	Low	N-His, N-S-tag		C-His
PET-30b(+)	Bacterial	T7 & Lac	Kanamycin	Low	N-His, N-S-tag		C-His
PET-30c(+)	Bacterial	T7 & Lac	Kanamycin	Low	N-His, N-S-tag		C-His
PET-31b(+)	Bacterial	T7 & Lac	Ampicillin	Low	N-KSI		C-His
pET-32a(+)	Bacterial	T7 & Lac	Ampicillin	Low	N-Trx		C-His
pET-32b(+)	Bacterial	T7 & Lac	Ampicillin	Low	N-Trx		C-His
pET-41a(+)	Bacterial	T7 & Lac	Kanamycin	Low	N-GST, N-His		C-S-tag, C-His
pET-41b(+)	Bacterial	T7 & Lac	Kanamycin	Low	N-GST, N-His		C-S-tag, C-His
pET-41c(+)	Bacterial	T7 & Lac	Kanamycin	Low	N-GST, N-His		C-S-tag, C-His
pET-42a(+)	Bacterial	T7 & Lac	Kanamycin	Low	N-GST, N-His		C-S-tag, C-His
pET-42b(+)	Bacterial	T7 & Lac	Kanamycin	Low	N-GST, N-His		C-S-tag, C-His
pET-42c(+)	Bacterial	T7 & Lac	Kanamycin	Low	N-GST, N-His		C-S-tag, C-His
pET-43.1a(+)	Bacterial	T7 & Lac	Ampicillin	Low	N-GST, N-His		C-S-tag, C-His
pET-43.1b(+)	Bacterial	T7 & Lac	Ampicillin	Low	N-GST, N-His		C-S-tag, C-His
pET-45b(+)	Bacterial	T7 & Lac	Ampicillin	Low	N-His		C-S-tag
pET-50b(+)	Bacterial	T7 & Lac	Kanamycin	Low	N-His, N-Nus		C-S-tag
pET-51b(+)	Bacterial	T7 & Lac	Ampicillin	Low	N-Strep		C-His
pET-52b(+)	Bacterial	T7 & Lac	Ampicillin	Low	N-Strep		C-His
pET-24a(+)-TEV	Bacterial	T7 & Lac	Kanamycin	Low	N-T7	TEV	C-His
pET-28a(+)-TEV	Bacterial	T7 & Lac	Kanamycin	Low	N-T7	TEV	C-His
pGEX-2TK	Bacterial	Tac	Ampicillin	Low	N-GST
pGEX-4T-1	Bacterial	Tac	Ampicillin	Low	N-GST
pGEX-4T-2	Bacterial	Tac	Ampicillin	Low	N-GST
pGEX-4T-3	Bacterial	Tac	Ampicillin	Low	N-GST
pGEX-5X-1	Bacterial	Tac	Ampicillin	Low	N-GST
pGEX-5X-2	Bacterial	Tac	Ampicillin	Low	N-GST
pGEX-5X-3	Bacterial	Tac	Ampicillin	Low	N-GST
pGEX-6P-1	Bacterial	Tac	Ampicillin	Low	N-GST
pGEX-6P-2	Bacterial	Tac	Ampicillin	Low	N-GST
pGEX-6P-3	Bacterial	Tac	Ampicillin	Low	N-GST
pMAL-c4x	Bacterial	Tac	Ampicillin	Low	N-MBP
pMAL-c5E	Bacterial	Tac	Ampicillin	Low	N-MBP
pMAL-c5x	Bacterial	Tac	Ampicillin	Low	N-MBP
pMAL-p5E	Bacterial	Tac	Ampicillin	Low	N-MBP
pMAl-p5g	Bacterial	Tac	Ampicillin	Low	N-MBP
pMAl-p5x	Bacterial	Tac	Ampicillin	Low	N-MBP
pQE-1	Bacterial	T5,lac	Ampicillin	Low	N-His
pQE-60	Bacterial	T5,lac	Ampicillin	Low			C-His
pGS-21a	Bacterial	T7,lac	Ampicillin	Low	N-His, N-GST
pETDuet-1	Bacterial	T7,lac	Ampicillin	Low	N-His
pCDFDuet-1	Bacterial	T7,lac	Streptomycin	Low	N-His
pRSFDuet-1	Bacterial	T7,lac	Kanamycin	Low	N-His
pCOLADuet-1	Bacterial	T7,lac	Kanamycin	Low	N-His
pGEX-4T-1-H(RBS)	Bacterial	Tac	Ampicillin	Low	N-GST
pGEX-4T-1-M(RBS)	Bacterial	Tac	Ampicillin	Low	N-GST
pGEX-5X-1-H(RBS)	Bacterial	Tac	Ampicillin	Low	N-GST
pGEX-5X-1-M(RBS)	Bacterial	Tac	Ampicillin	Low	N-GST
pGEX-6P-1-H(RBS)	Bacterial	Tac	Ampicillin	Low	N-GST
pGEX-6P-1-M(RBS)	Bacterial	Tac	Ampicillin	Low	N-GST
pMAL-c4x-1-H(RBS)	Bacterial	Tac	Ampicillin	Low	N-MBP
pMAL-c4x-1-M(RBS)	Bacterial	Tac	Ampicillin	Low	N-MBP
pBacPAK8	Baculovirus	polyhedrin	Ampicillin	High
pBacPAK9	Baculovirus	polyhedrin	Ampicillin	High
pAcG2T	Baculovirus	polyhedrin	Ampicillin	High	N-GST
pAcHLT A	Baculovirus	polyhedrin	Ampicillin	High	N-His
pAcHLT B	Baculovirus	polyhedrin	Ampicillin	High	N-His
pAcHLT C	Baculovirus	polyhedrin	Ampicillin	High	N-His
pAcGHLT A	Baculovirus	polyhedrin	Ampicillin	High	N-His
pAcGHLT B	Baculovirus	polyhedrin	Ampicillin	High	N-His
pAcGHLT C	Baculovirus	polyhedrin	Ampicillin	High	N-His
pAcSG2	Baculovirus	polyhedrin	Ampicillin	High
pBAC-1	Baculovirus	polyhedrin	Ampicillin	High	C-His
pFastBac1	Baculovirus	polyhedrin	Ampicillin	High
pFastBacHT-A	Baculovirus	polyhedrin	Ampicillin	High	N-His	TEV
pFastBacHT-B	Baculovirus	polyhedrin	Ampicillin	High	N-His	TEV
pFastBacHT-C	Baculovirus	polyhedrin	Ampicillin	High	N-His	TEV
pFastBac-Dual	Baculovirus	polyhedrin, p10	Ampicillin	High
pAO815	Pichia	AOX1	Ampicillin	High
pPIC 3.5k	Pichia	AOX1	Ampicillin	High
pPIC9	Pichia	AOX1	Ampicillin	High	N-α-factor secretion signal
pPIC9K	Pichia	AOX1	Ampicillin	High
pPICZA	Pichia	AOX1	Zeocin	High			C-Myc, C-His
pPICZB	Pichia	AOX1	Zeocin	High			C-Myc, C-His
pPICZC	Pichia	AOX1	Zeocin	High			C-Myc, C-His
pPICZalphaA	Pichia	AOX1	Zeocin	High	N-α-factor secretion signal		C-Myc, C-His
pPICZalphaB	Pichia	AOX1	Zeocin	High	N-α-factor secretion signal		C-Myc, C-His
pPICZalphaC	Pichia	AOX1	Zeocin	High	N-α-factor secretion signal		C-Myc, C-His
pESC-TRP	S. cerevisiae	GAL1, GAL10	Ampicillin	High			C-DYKDDDDK, C-Myc
pESC-URA	S. cerevisiae	GAL1, GAL10	Ampicillin	High			C-DYKDDDDK, C-Myc
pESC-HIS	S. cerevisiae	GAL1, GAL10	Ampicillin	High			C-DYKDDDDK, C-Myc
pESC-LEU	S. cerevisiae	GAL1, GAL10	Ampicillin	High			C-DYKDDDDK, C-Myc

 

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