배 경
- 단백질 S(PROS1)은 당단백질로 응고, 세포자멸사, 암발달 및 진행 선천성 면역반응을 포함하는 여러 생물학적 과정에 관련되어 있으며, S1에 결합하는 알려진 receptor는 ACE2, angiotensin-converting enzyme 2, DPP4, CEACAM 등입니다.
- 코로나바이러스의 Spike (S) glycoprotein은 감염과정시 숙주세포에 바이러스가 결합하는데 필수적인 것으로 알려져 있습니다.
- Spike 단백질은 S1과 S2라는 두개의 소단위를 포함하는 type I transmembrane protein 으로, S1은 주로 세포 표면 수용체를 인식하는 역할을 하는 수용체 결합 도메인 (Receptor binding domain, RBD)를 포함하고 S2는 막 융합에 필요한 기본요소를 포함합니다. S 단백질은 중화항체 및 T 세포 반응 유도, 보호 면역에 중요한 역할을 합니다.
- Omicron variant (오미크론 변이)는 WHO에 보고된 후 variant of concern (VOC)로 지정될 만큼 전염성이 강한 변이체로, Delta 변이에 비해 두배 이상의 강한 전파력을 가지고 있습니다.
- 오미크론 변이 spike protein은 mutation, deletion, insertion등 약 30개 amino acid에 변화가 있으며 이로인해 전파력, 면역 저항성, 항체치료제에 대한 내성을 유발합니다.
- G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493K, G496S, Q498R, N501Y, Y505H에 mutation을 갖는 오미크론 변이 RBD protein을 제공합니다.
오미크론 map
주요 제품
SARS-CoV-2 Omicron Variant Recombinant Proteins
|
Product |
Cat. No. |
Source |
Fusion Tag |
Sequence |
|
SARS-CoV-2
(COVID-19) Omicron Variant (B.1.1.529) Spike RBD Protein |
21-801
21-844
95-128 |
Mammalian Cells
HEK 293 |
C-His/C-Fc/His-Avi |
Arg319-Phe541 (G339D, S371L, S373P, S375F, K417N, N440K, G446S,
S477N, T478K, E484A, Q493K/R, G496S,Q498R, N501Y, Y505H.) |
|
SARS-CoV-2 (COVID-19) Omicron Variant (B.1.1.529)
Spike S1 Protein |
95-129
21-846 |
HEK 293
Mammalian Cells |
His-Avi |
Val 16 - Arg 685 (A67V, HV69-70del, T95I, G142D, VYY143-145del,
N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S,
S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y,
N679K, P681H) |
|
SARS-CoV-2 (COVID-19) Omicron Variant (B.1.1.529)
Spike Trimer Protein |
21-845 |
Mammalian Cells |
C-his |
Ser13 - Lys1208 (A67V, HV69-70del, T95I, G142D, VYY143-145del,
N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S,
S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y,
N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F) |
|
SARS-CoV-2 (COVID-19) Omicron Variant (B.1.1.529)
Nucleocapsid Protein |
21-850 |
Mammalian Cells |
C-his |
Met1-Ala419 (P13L, ERS31-33 del, R203K, G240R) |
SARS-CoV-2 Omicron Variant (B.1.1.529) Spike RBD Recombinant Protein (#21-801)
| SPECIES: | SARS-CoV-2 |
| SOURCE SPECIES: | Mammalian cells |
| SEQUENCE: | RBD with mutations at G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493K, G496S, Q498R, N501Y, Y505H. |
| FUSION TAG: | His Tag |
| APPLICATIONS: | Immunogen |
| PURITY: | >90% as determined by SDS-PAGE |
| PHYSICAL STATE: | Lyophilized |
| BUFFER: | Supplied as lyophilized from PBS, pH 7.5. Please reconstitute the protein with ddH2O to the concentration with 1mg/ml. |
| STORAGE CONDITIONS: | Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Store at 2 to 8 ˚C for one week .Store at -20 to -80 ˚C for twelve months from the date of receipt. |
| ALTERNATE NAMES: | Spike,S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD |
| ACCESSION NO.: | P0DTC2(G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493K, G496S, Q498R, N501Y, Y505H) |
SARS-CoV-2 (COVID-19) Omicron Variant (B.1.1.529) Spike RBD Recombinant Protein
SARS-CoV-2 (COVID-19) Omicron Variant Spike S1 (His-Avi Tag) Recombinant Protein (#95-129)
| SPECIES: | SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Protein |
| SOURCE SPECIES: | HEK293 cells |
| SEQUENCE: | Val 16 - Arg 685 (A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H)-His-Avi |
| FUSION TAG: | His-Avi tag at C-terminus |
| TESTED APPLICATIONS: | ELISA, WB |
| APPLICATIONS: | This recombinant protein can be used for WB and ELISA. For research use only. |
| BIOLOGICAL ACTIVITY: | N/A |
| PURITY: | > 90% as determined by Bis-Tris PAGE |
| MOLECULAR WEIGHT: | 120 kD |
| PHYSICAL STATE: | Liquid |
| BUFFER: | PBS (pH 7.4) |
| STORAGE CONDITIONS: | Store in working aliquots at -70˚C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
| ALTERNATE NAMES: | S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD, Omicron variant spike RBD |
| ACCESSION NO.: | QHD43416.1 |
| OFFICIAL SYMBOL: | Surface glycoprotein |
| GENE ID: | 43740578 |
SARS-CoV-2 (COVID-19) Omicron Variant (B.1.1.529) Spike Trimer Recombinant Protein (#21-845)
| SPECIES: | SARS-CoV-2 |
| SOURCE SPECIES: | Mammalian cells |
| SEQUENCE: | The target gene encoding Ser13-Lys1208. Mutation points A67V, H69del, V70del, T95I, G142D, V143del, Y144del, Y145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F |
| FUSION TAG: | C-His Tag |
| APPLICATIONS: | Immunogen |
| PURITY: | >90% as determined by SDS-PAGE |
| MOLECULAR WEIGHT: | 139.62 kDa |
| PHYSICAL STATE: | Lyophilized |
| BUFFER: | Supplied as solution form in PBS, pH7.5 or lyophilized from PBS, pH7.5 |
| STORAGE CONDITIONS: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 ˚C for one week . Store at -20 to -80 ˚C for twelve months from the date of receipt. |
| ALTERNATE NAMES: | Spike,S protein Trimer,Spike glycoprotein Receptor-binding domain,S glycoprotein Trimer,Spike protein Trimer |
| ACCESSION NO.: | UFO069279.1 |
SARS-CoV-2 Spike P681H Antibody [7C11H11] (Omicron, Alpha Variant) (#PM-9375)
| HOST SPECIES: | Mouse |
| SPECIES REACTIVITY: | Virus |
| HOMOLOGY: | Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (25%) |
| IMMUNOGEN: | Anti-SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike P681H antibody (PM-9375) was raised against a peptide corresponding to 12 amino acids near the center of SARS-CoV-2 Alpha variant (B.1.1.7) Spike protein.
The immunogen is located within 650-700 amino acids of SARS-CoV-2 Alpha Variant (B.1.1.7, UK) Spike protein. |
| TESTED APPLICATIONS: | ELISA, WB |
| APPLICATIONS: | WB: 1 μg/mL.
Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike antibody can specifically detect SARS-CoV-2 Alpha Variant (B.1.1.7, UK) S1 protein, but not SARS-CoV-2 WT Spike S1 protein by ELISA and WB. It can also detect mutant peptide (681H), but not WT peptide (681P). All other applications and species not yet tested. |
| SPECIFICITY: | May cross-react with several virus of interest (VOI) variant lineages that contains P681H mutation, including B.11.318, B.1.621, B.1.621.1, P.3. But all of these lineages are rarely present in current pandemic. |
| PURIFICATION: | SARS-CoV-2 (COVID-19) Alpha Variant (B.1.1.7, UK) Spike P681H Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Monoclonal |
| ISOTYPE: | IgG2b, Kappa |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
SARS-CoV-2 (COVID-19) Spike G142D Δ143-145VYY Antibody (Omicron) (#9793)
| HOST
SPECIES: |
Rabbit |
| HOMOLOGY: |
Predicted
reactivity based on immunogen sequence: SARS-CoV Spike proteins: (33.3%) |
| IMMUNOGEN: |
Anti-SARS-CoV-2
Omicron variant specific Spike (G142D Δ143-145VYY) antibody(9793) was raised
against a peptide corresponding to 12 amino acids near the amino terminal
domain of SARS-CoV-2 Omicron variant (B.1.1.529) Spike protein.
The immunogen is located within 130-180 amino acids of SARS-CoV-2 Omicron
variant (B.1.1.529) Spike protein. |
| CONJUGATE: |
Unconjugated |
| TESTED APPLICATIONS: |
E,
WB |
| APPLICATION NOTE: |
E,
WB |
| SPECIFICITY: |
It
can only detect SARS-CoV-2 Omicron Variant (B.1.1.529) Spike protein and does
not cross-react with the spike proteins of other variants. |
| PURIFICATION: |
Anti-SARS-CoV-2
Omicron Variant Spike antibody is affinity chromatography purified via
peptide column. |
| CLONALITY: |
Polyclonal |
| ISOTYPE: |
IgG |
| PHYSICAL STATE: |
Liquid |
| BUFFER: |
Anti-SARS-CoV-2
Omicron Variant Spike antibody is supplied in PBS containing 0.02% sodium
azide. |
| CONCENTRATION: |
1
mg/ml |
Left : SARS-Cov-2 Omicron Variant Spike Antibodies Specifically Detect Omicron Variant Spike S1 Protein in an ELISA
Coating Antigen: SARS-CoV-2 spike S1 proteins of WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Omicron Variant Spike antibody, 9793, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.
Right : WB Validation of of Omicron Variant Spike Antibodies with SARS-CoV-2 Variant Spike S1 Proteins
Loading: 30 ng of SARS-CoV-2 spike S1 proteins, including WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529),Detection Antibodies: SARS-CoV-2 Omicron Variant Spike antibody, 9793, 2 μg/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.SARS-CoV-2 Omicron Variant Spike antibody (9793) specifically detects Omicron variant spike S1 protein (95-129), but not spike S1 protein of WT (10-300) and all other variants.
SARS-CoV-2 (COVID-19) Spike Q493R G496S Q498R N501Y Y505H Antibody (Omicron)(#9805)
| HOST
SPECIES: |
Rabbit |
| HOMOLOGY: |
Predicted
reactivity based on immunogen sequence: SARS-CoV Spike proteins: (55%) |
| IMMUNOGEN: |
Anti-SARS-CoV-2
Spike Q493R G496S Q498R N501Y Y505H (Omicron) antibody(9805) was raised
against a peptide corresponding to 20 amino acids in the receptor binding
domain of SARS-CoV-2 Omicron variant (B.1.1.529) Spike protein.
The immunogen is located within 480-530 amino acids of SARS-CoV-2 Omicron
variant (B.1.1.529) Spike protein. |
| CONJUGATE: |
Unconjugated |
| TESTED APPLICATIONS: |
E,
WB |
| APPLICATION NOTE: |
WB:
2 μg/mL.
Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Omicron
variant Spike antibody specifically detects Spike S1 protein of Omicron
Variant (B.1.1.529), but not WT and other variant by ELISA and WB. All other
applications and species not yet tested. |
| SPECIFICITY: |
It
can only detect SARS-CoV-2 Omicron Variant (B.1.1.529) Spike protein and does
not cross-react with the spike proteins of other variants. |
| PURIFICATION: |
Anti-SARS-CoV-2
Omicron Variant Spike antibody is affinity chromatography purified via
peptide column. |
| CLONALITY: |
Polyclonal |
| ISOTYPE: |
IgG |
| PHYSICAL STATE: |
Liquid |
| BUFFER: |
Anti-SARS-CoV-2
Omicron Variant Spike antibody is supplied in PBS containing 0.02% sodium
azide. |
| CONCENTRATION: |
1
mg/mL |
Left : SARS-Cov-2 Omicron Variant Spike Antibodies Specifically Detect Omicron Variant Spike S1 Protein & Spike RBD Protein in an ELISA, respectively
Right : WB Validation of of Omicron Variant Spike Antibodies with SARS-CoV-2 Variant Spike S1 Proteins