SPINeasy DNA Kit for Tissue

제품 상세

SPINeasy DNA Kit for Tissue는 동물 조직에서 spin column technology를 이용하여 편리하게 genomic DNA를 추출하는 키트 입니다.

프린트 제품 문의

특징

지방이 많고 질긴 조직 (설치류 꼬리, 귀 펀치), 섬유질 조직 (심장, 근육) 등과 같이 용해가 어려운 샘플에서 30분만에 높은 수율의 DNA를 추출합니다.
Lysis buffer에는 RNA 오염 가능성을 최소화하는 RNase A가 포함되어 있습니다.
Buffer TD2를 사용한 후속 처리를 통해 DNA가 컬럼 S에 선택적으로 결합할 수 있습니다.
여러 유형의 조직에서 추출된 genome DNA는 PCR을 억제하지 않으며, long fragment PCR, qPCR, restriction digestion, sequencing등의 실험에 사용할 수 있습니다.
Lysing Matrix 포함, 혹은 비포함 옵션을 선택하실 수 있습니다.

구성

SPINeasy DNA Kit for Tissue With Lysing matrix (cat# 116558050)	SPINeasy DNA Kit for Tissue Without Lysing matrix (cat# 116559050)
Lysing Matrix M (50 ea) Equilibration Buffer (12 mL) Buffer TD1 (15 mL) Buffer TD2 (30 mL) Buffer TD3 (18 mL) Buffer TD4 (6 mL) Buffer TD5 (15 mL) Proteinase K (1.2 mL) RNase A (250 µL) Column S (50 ea) Collection Tube (150 ea) Elution Tube (50 ea)	Equilibration Buffer (12 mL) Buffer TD1 (15 mL) Buffer TD2 (30 mL) Buffer TD3 (18 mL) Buffer TD4 (6 mL) Buffer TD5 (15 mL) Proteinase K (1.2 mL) RNase A (250 µL) Column S (50 ea) Collection Tube (150 ea) Elution Tube (50 ea)

참고 데이터

Figure 1: Comparison of quality and quantity of gDNA extracted from various sample types using SPINeasy® DNA Kit for Tissue and Competitor Q. The gDNA yield, A260/A230 and A260/A280 ratio (from 15 mg of respective tissues) were measured with a spectrophotometer. The virtual gel image and DNA Integrity Number (DIN, in green, yellow, or red) were analyzed using Agilent Tapestation 4150.

Figure 2: Amplifiability of DNA extracted with SPINeasy® DNA Kit for Tissue (with Lysing Matrix)

Upper panel: Agarose gel electrophoresis of gDNA isolated from various rabbit/mouse tissues using SPINeasy® DNA Kit for Tissue (with Lysing Matrix). One microliter of extracted gDNA was amplified using rabbit or mouse-specific α-actin primers (1kb product). Reactions without DNA served as negative controls (NC).

Lower panel: Comparison of threshold cycles (Ct) of qPCR when amplification was performed using equal quantity of chicken liver, beef, and fish gill gDNA (25 ng). Targets were amplified with SYBR green.

Figure 3: Amplifiability of DNA extracted with SPINeasy® DNA Kit for Tissue (Without Lysing Matrix)

Upper panel: Agarose gel electrophoresis of PCR products amplified from gDNA isolated from various rabbit/mouse tissues using SPINeasy® DNA Kit for Tissue (Without Lysing Matrix). One microliter of extracted gDNA was amplified using rabbit or mouse-specific α-actin primers (1kb product). Reactions without DNA served as negative controls (NC).

Lower panel: Comparison of threshold cycles (Ct) of qPCR when amplification was performed using equal quantity of chicken liver, beef, and fish gill gDNA (25 ng) extracted with SPINeasy® DNA Kit and Competitor Q. Targets were amplified with SYBR green.

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
	Product	Cat.No.	Size	Maker	Qty	Data Sheet	MSDS
	SPINeasy DNA Kit for Tissue With Lysing matrix	116558050	50 preps	MPBIO
	SPINeasy DNA Kit for Tissue Without lysing matrix	116559050	50 preps	MPBIO

1
Maker	MPBIO
Cat.No.	116558050
Product	SPINeasy DNA Kit for Tissue With Lysing matrix
Size	50 preps
Qty
Data Sheet

2
Maker	MPBIO
Cat.No.	116559050
Product	SPINeasy DNA Kit for Tissue Without lysing matrix
Size	50 preps
Qty
Data Sheet

자료

웨비나/Video

제품 문의

제품명

이름*

직급*

소속/부서명*

교수님명*

이메일*

핸드폰 번호*

제목

내용

개인 정보 수집 및 이용 동의

SPINeasy DNA Kit for Tissue | 고마바이오텍