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Ion Assay Kit | Cofactor Assay Kit 이미지

Ion Assay Kit | Cofactor Assay Kit

Calcium, NAD+/NADH, NADP+/NADPH, Zinc, Chloride, Chromium, Copper, Iron, Magnesium등 ion 및 mineral cofactor를 측정하는 Assay Kit을 소개합니다.

Calcium Assay Kit (#MET-5121) 

  

  • Calcium은 GPCR 시그널링에서는 signal transduction pathway를 유도하는 2차 메신저로서, 뉴런, 심장, 근육세포 등에서는 calcium channel을 통한 action potential에 관여하는 중요한 이온입니다. 또한 많은 enzyme의 cofactor로서 역할을 하며, hormone level 조절에 관여하기도 합니다.
  • Calcium Assay Kit은 컬러 probe를 이용하여 샘플내 칼슘의 양을 측정하는 제품입니다. 
  • 민감도 : 15.6uM calcium 측정
  • 구성 :
    • Calcium standard
    • Assay buffer
    • Colorimetric Probe


 

NAD+/NADH Assay Kit (#MET-5014, MET-5030)

  • NAD는 adenine과 nicotinamide로 구성되며 phosphate group에 의해 연결되어 있습니다. 모든 living cell에서 발견되며, glycolysis나 citric acid cycle에서 electron를 전달하는 역할을 합니다. NAD는 전자 수용체 역할을 하는 산화된 형태 (NAD+)와 전자를 제공하는 환원된 형태 (NADH) 사이를 순환합니다. 
  • NAD+/NADH Assay kit은 cell과 tissue에 존재하는 NAD+와 NADH를 검출합니다.
  • 컬러방법(MET-5014)와 형광방법(MET-5030)을 제공합니다.
  • 구성 : Probe, NAD Cycling Substrate, Assay Buffer, Extraction Buffer, NAD Cycling Enzyme, NAD+Standard 



NAD+/NADH Assay Kit (#MET-5014, MET-5030)




NADP+/NADPH Assay Kit (#MET-5018, MET-5031)

  • NADP는 metabolic redox와 cell signaling에 관여하는 cofactor로, 환원형태(NADPH)와 전자 수용 산화형태(NADP+)를 순환하며 전자를 전달합니다. 
  • NADP+/NADPH Assay Kit는 cell, tissue lysate로부터 NADP+와 NADPH를 검출합니다. 
  • 컬러방법(MET-5018)과 형광방법(MET-5031)을 제공합니다.



Zinc Assay Kit (#MET-5138)

  • 생물학적 시료에 존재하는 아연의 총량을 측정하는 컬러분석 킷트입니다.
  • 단백질로부터 유리된 아연은 컬러 시약과 반응하여 red color를 발색하고 96well spectrophotometric microtiter plate에서 측정됩니다. 
  • 민감도 : ~0.4 µM zinc 검출
  • 구성 : 
    • Color Reagent
    • Zinc Reagent 
    • Zinc Standard
    • Deproteinizing Solution
Zinc Assay Kit (#MET-5138)



사용 논문

MET-5121  Graybill, P.M. et al. Single Cell Forces after Electroporation. ACS Nano. doi: 10.1021/acsnano.0c07020.

MET-5014,

MET-5030

 
    1. Kotla, S. et al. (2021). Nucleus-mitochondria positive feedback loop formed by ERK5 S496 phosphorylation-mediated poly (ADP-ribose) polymerase activation provokes persistent pro-inflammatory senescent phenotype and accelerates coronary atherosclerosis after chemo-radiation. Redox Biol. doi: 10.1016/j.redox.2021.102132 (#MET-5030).
    2. Yumnam, S. et al. (2020). Downregulation of dihydrolipoyl dehydrogenase by UVA suppresses melanoma progression via triggering oxidative stress and altering energy metabolism. Free Radic Biol Med. doi: 10.1016/j.freeradbiomed.2020.11.037 (#MET-5014).
    3. Wahba, N.S. et al. (2019). Vitamin D3 potentiates the renoprotective effects of vildagliptin in a rat model of fructose/salt-induced insulin resistance. Eur J Pharm Sci. doi: 10.1016/j.ejps.2019.105196 (#MET-5014).
    4. Maurer, S. et al. (2019). Investigation of fatty aldehyde and alcohol synthesis from fatty acids by αDox- or CAR-expressing Escherichia coli. J Biotechnol. pii: S0168-1656(19)30828-4. doi: 10.1016/j.jbiotec.2019.08.011 (#MET-5014).
    5. Kawaguchi, A.T. et al. (2019). Artificial Oxygen Carrier Improves Fatigue-Resistance in Slow-muscle but not in Fast-muscle in a Rat in situ Model. Artif Organs. doi: 10.1111/aor.13535 (#MET-5030).

MET-5018,

MET-5031

 
    1. Ogawa, K. et al. (2020). Forced expression of miR-143 and -145 in cardiomyocytes induces cardiomyopathy with a reductive redox shift. Cell Mol Biol Lett25:40. doi: 10.1186/s11658-020-00232-x (#MET-5018).
    2. Liu, J. et al. (2020). Taurine protects against cardiac dysfunction induced by pressure overload through SIRT1–p53 activation. Chem Biol Interact. doi: 10.1016/j.cbi.2020.108972 (#MET-5018).


주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
Calcium Assay Kit MET-5121 250 assays CELL BIOLABS
Calcium Assay Kit (Colorimetric) ARG82142 500 tests ARIGO
Chloride Assay Kit (Colorimetric) ARG82143 250 tests ARIGO
Chromium Assay Kit (Colorimetric) ARG82145 250 tests ARIGO
Copper Assay Kit (Colorimetric) ARG82148 250 tests ARIGO
Direct Total Iron colorimetric assay (Serum etc), 480 tests 1230-5 1 kit ALPHA DIAGNOSTICS
Direct Total Iron colorimetric assay (Serum etc), 96 wells 1230 1 kit ALPHA DIAGNOSTICS
Magnesium Assay Kit (Colorimetric) ARG82177 250 tests ARIGO
NAD / NADH Assay Kit ARG82283 96 wells ARIGO
NAD+/NADH Assay Kit MET-5014 100 assays CELL BIOLABS
NAD+/NADH Assay Kit (Fluorometric) MET-5030 100 assays CELL BIOLABS
NADP+/NADPH Assay Kit MET-5018 100 assays CELL BIOLABS
NADP+/NADPH Assay Kit (Fluorometric) MET-5031 100 assays CELL BIOLABS
Nori Rat NADH Dehydrogenase ELISA Kit GR118317 96 wells GENORISE
QuantiChrom™ Calcium Assay Kit DICA-500 500 assays BIOASSAY SYSTEMS
QuantiChrom™ Chloride Assay Kit DICL-250 250 assays BIOASSAY SYSTEMS
QuantiChrom™ Chromium Assay Kit DCRM-250 250 assays BIOASSAY SYSTEMS
QuantiChrom™ Copper Assay Kit DICU-250 250 assays BIOASSAY SYSTEMS
QuantiChrom™ Iron Assay Kit DIFE-250 250 assays BIOASSAY SYSTEMS
QuantiChrom™ Magnesium Assay Kit DIMG-250 250 assays BIOASSAY SYSTEMS
QuantiChrom™ Zinc Assay Kit DIZN-250 250 assays BIOASSAY SYSTEMS
Zinc Assay Kit (Colorimetric) MET-5138 100 assays CELL BIOLABS
Zinc Assay Kit (Colorimetric) ARG82199 250 tests ARIGO
전체보기
Maker
CELL BIOLABS
Cat.No.
MET-5121
Product
Calcium Assay Kit
Size
250 assays
Qty
Data Sheet
MSDS
Maker
ARIGO
Cat.No.
ARG82142
Product
Calcium Assay Kit (Colorimetric)
Size
500 tests
Qty
Data Sheet
Maker
ARIGO
Cat.No.
ARG82143
Product
Chloride Assay Kit (Colorimetric)
Size
250 tests
Qty
Data Sheet
Maker
ARIGO
Cat.No.
ARG82145
Product
Chromium Assay Kit (Colorimetric)
Size
250 tests
Qty
Data Sheet
Maker
ARIGO
Cat.No.
ARG82148
Product
Copper Assay Kit (Colorimetric)
Size
250 tests
Qty
Data Sheet
Maker
ALPHA DIAGNOSTICS
Cat.No.
1230-5
Product
Direct Total Iron colorimetric assay (Serum etc), 480 tests
Size
1 kit
Qty
Data Sheet
Maker
ALPHA DIAGNOSTICS
Cat.No.
1230
Product
Direct Total Iron colorimetric assay (Serum etc), 96 wells
Size
1 kit
Qty
Data Sheet
Maker
ARIGO
Cat.No.
ARG82177
Product
Magnesium Assay Kit (Colorimetric)
Size
250 tests
Qty
Data Sheet
Maker
ARIGO
Cat.No.
ARG82283
Product
NAD / NADH Assay Kit
Size
96 wells
Qty
Data Sheet
Maker
CELL BIOLABS
Cat.No.
MET-5014
Product
NAD+/NADH Assay Kit
Size
100 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
MET-5030
Product
NAD+/NADH Assay Kit (Fluorometric)
Size
100 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
MET-5018
Product
NADP+/NADPH Assay Kit
Size
100 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
MET-5031
Product
NADP+/NADPH Assay Kit (Fluorometric)
Size
100 assays
Qty
Data Sheet
MSDS
Maker
GENORISE
Cat.No.
GR118317
Product
Nori Rat NADH Dehydrogenase ELISA Kit
Size
96 wells
Qty
Maker
BIOASSAY SYSTEMS
Cat.No.
DICA-500
Product
QuantiChrom™ Calcium Assay Kit
Size
500 assays
Qty
Data Sheet
MSDS
Maker
BIOASSAY SYSTEMS
Cat.No.
DICL-250
Product
QuantiChrom™ Chloride Assay Kit
Size
250 assays
Qty
Data Sheet
MSDS
Maker
BIOASSAY SYSTEMS
Cat.No.
DCRM-250
Product
QuantiChrom™ Chromium Assay Kit
Size
250 assays
Qty
Data Sheet
MSDS
Maker
BIOASSAY SYSTEMS
Cat.No.
DICU-250
Product
QuantiChrom™ Copper Assay Kit
Size
250 assays
Qty
Data Sheet
MSDS
Maker
BIOASSAY SYSTEMS
Cat.No.
DIFE-250
Product
QuantiChrom™ Iron Assay Kit
Size
250 assays
Qty
Data Sheet
MSDS
Maker
BIOASSAY SYSTEMS
Cat.No.
DIMG-250
Product
QuantiChrom™ Magnesium Assay Kit
Size
250 assays
Qty
Data Sheet
MSDS
Maker
BIOASSAY SYSTEMS
Cat.No.
DIZN-250
Product
QuantiChrom™ Zinc Assay Kit
Size
250 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
MET-5138
Product
Zinc Assay Kit (Colorimetric)
Size
100 assays
Qty
Data Sheet
MSDS
Maker
ARIGO
Cat.No.
ARG82199
Product
Zinc Assay Kit (Colorimetric)
Size
250 tests
Qty
Data Sheet
전체보기

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