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제품 상세
Direct PCR Buffer 이미지

Direct PCR Buffer

EzWay Direct PCR Buffer는 Whole blood, Mouse tail, Tissue 등과 같은 Sample에서 DNA 정제없이 바로 PCR을 수행할 수 있습니다.

프린트 제품 문의

특징

  

  • PCR전에 DNA를 purification할 필요가 없이 바로 PCR을 수행할 수 있습니다.
    • DNA purification에 드는 시간을 절약해 드립니다.
    • 번거로운 스텝을 줄여주므로 편리합니다.
    • 샘플핸들링시 발생할지도 모르는 contamination 위험으로부터 안전합니다.
    • DNA purification중에 발생하는 샘플 loss의 염려가 없습니다.
    • 여러조작이 필요없으므로, 다량의 샘플을 한번에 PCR할 수 있습니다. 

  • Whole blood 및 다양한 샘플에 Direct PCR을 적용할 수 있습니다. 
    • Blood (Fresh, heparinized, EDTA-treated, Citrate-treated, dried blood on papers)
    • Animal (mouse) tails
    • Tissues (fresh, frozen or paraffinized), tissue microarray
    • Cultured cells
    • Buccal cells, saliva
    • Hair root, sperm
    • Plant
    • Bacteria 등

 

  • 어떤 DNA Polymerase를 이용해도 Direct PCR이 가능합니다. 
    • 여러 회사의Taq과 호환됩니다.
    • Taq, Pfu, Pwo, Tth등의 다양한 DNA polymerase 뿐만 아니라, chemically modified Hot start DNA polymerase에도 호환됩니다.

 

  • Hot start 효과를 가지므로, background없는 깨끗한 특이 밴드만을 잡아낼 수 있습니다. 
    • lysis 과정을 거치지 않는 blood와 같은 샘플의 경우, PCR 시작 후 온도가 상승하면서 blood cell이 깨지고 DNA가 유리되면 이때 primer가 타겟 DNA site에 반응할 수 있으므로 비특이반응 우려로부터 안전합니다. 


적용

  • Forensic science
  • Viral infection, Molecular diagnostic test
  • Multiplex PCR. SNP detection, PCR-RFLP
  • Single cell diagnostic
  • Sequencing, Cloning
  • Blood banking, Identity testing
  • Laboratory automatic PCR
 

구성 

  • Direct PCR Buffer : EzWay™ Direct PCR Buffer only
  • Direct PCR Buffer Set  :  EzWay™ Direct PCR Buffer, Lysis Buffer I & II,  Magic Buffer (For high GC contents)


데이터


Fig.1 Direct PCR from whole blood
PCR amplifications were performed using 20 ng human genomic DNA or 1 ul Heparin-treated whole blood in a 50ul PCR reaction volume. EzWayTM Direct PCR Buffer can amplify directly up to 2kb amplicon and achieve clear amplification than DNA PCR. It means that direct PCR amplification has hot start function even without the help of hot start enzyme because the cells are disrupted in initial heating stage, then the exposed DNA templates meet primers instantly at higher temperature than Tm.



Fig.2 Direct PCR from cultured cell
Direct PCR was performed with U-138 brain tumor cell. Cells were lysed with the EzWayTM Direct Lysis Buffer II first and incubated at room temperature for 10 minutes. 1ul of the supernatant was added directly to 20ul PCR reaction mixtures.



Fig.3 Direct PCR from mouse tail
PCR template was obtained from mouse albino tail by incubation at 56°C for 10 minutes with the EzWayTM Direct Lysis Buffer I and Proteinase K (PK). PK was removed by heating at 95°C for 5 minutes, then,1ul of the supernatant was added directly to 20ul PCR reaction mixtures. The mouse albino mutation was identified by rapid lysis and PCR-RFLP using EzWayTM Direct PCR Buffer. A point mutation in the tyrosinase gene of BALB/c albino mouse at position 85 (cystein → serine) creats an additional DdeI site and this mutation is actually present in BALB/c genomic DNA.
 BALB/c strain : 20, 113, 35, 130 bp
 C57BL/6 strain : 20, 113, 165 bp



Fig.4 Direct PCR from Plant
Electropherogram with Arabidopsis leaf lysate and purified genomic DNA. Four leaves of Arabidopsis were mixed with 100 ul of 1X Direct Lysis Buffer I, ground with a pestle (+) or not ground (-), followed by incubation at 80°C for 2 hours. After heat treatment, these lysates were amplified with conventional PCR buffer (Lane 1-4) and EzWayTM Direct PCR Buffer (Lane 5-8). Amplification of genomic DNA from two ecotypes,Col (lane 9) and Ler (lane 10), was identified with amplicon sizes of 0.85 and 1.0 kb, respectively. Thus, one sample (lane 1, 3, 5, and 7) was a Col homozygote, and the other (lane 2, 4, 6, and 8) was a Col/Ler heterozygote.



Fig.5 Melting curve analysis of duplex real-time PCR amplification of S. flexneri and S. typhimurium
The virA and invA gene fragments were amplified simultaneously from fecal samples spiked with equal amounts of S. flexneri and S. typhimurium in the presence of EzWayTM Direct PCR Buffer.
Tms were resolvable between the two products. Tm was 82°C and 87°C for virA and invA, respectively. Specific products are visible at 215 bp and 284 bp for each dilution.


주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
EzWay Direct PCR Buffer (5x) K0568001 500ul LABIS KOMA
EzWay Direct PCR Buffer Set (5x) K0568002 1set LABIS KOMA
Maker
LABIS KOMA
Cat.No.
K0568001
Product
EzWay Direct PCR Buffer (5x)
Size
500ul
Qty
Data Sheet
Maker
LABIS KOMA
Cat.No.
K0568002
Product
EzWay Direct PCR Buffer Set (5x)
Size
1set
Qty
Data Sheet

추천제품

관련제품

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
EzWay Direct Taq PCR MasterMix (2x) K0568010 1ml LABIS KOMA
EzWay Direct Taq PCR MasterMix w/o dye (2x) K0568020 1ml LABIS KOMA
EzWay Direct ApoE Genotyping Kit K0568500 50T LABIS KOMA
EzWay Direct Hot SYBRGreen qMasterMix K0569021 1ml LABIS KOMA
EzWay Direct Hot Taq PCR MasterMix K0568210 1ml LABIS KOMA
EzWay Direct Hot Universal qMasterMix K0569020 1ml LABIS KOMA
EzWay Direct Multiplex PCR MasterMix (2x) K0567840 1ml LABIS KOMA
EzWay Direct PCR Buffer Set (5x) K0568002 1set LABIS KOMA
EzWay Mouse Tail Direct PCR Kit K0568600 1set LABIS KOMA
EzWay Plant Direct PCR Kit K0568700 1set LABIS KOMA

자료

  • 래비스고마 PRODUCT & SERVICE

    래비스고마 PRODUCT & SERVICE 이미지

    [LABISKOMA]

    ELISA & Assay / Precast Gel / Electrophoresis / PCR / Multiplex

웨비나/Video

TOP

제품 문의

 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
Maker
Cat.No.
Product
Size
Qty
Data Sheet
MSDS

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