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제품 상세
RNA 정량 | AccuBlue® Broad Range RNA Quantitation Kit 이미지

RNA 정량 | AccuBlue® Broad Range RNA Quantitation Kit

AccuBlue® Broad Range RNA Quantitation Kit은 5-1000 ng 범위의 RNA를 정량할 수 있으며 NGS, RT-PCR 실험을 위한 RNA 준비에 효과적입니다.

프린트 제품 문의

AccuBlue® Broad Range RNA Quantitation Kit (#31073)  

 

  • Widest linear range의 RNA를 정량합니다.
  • 형광 microplate reader 및 Qubit® fluorometer로 측정합니다.
  • mammalian cell RNA의 정확한 측량을 위해 mammalian RNA standard를 제공합니다.
  • NGS, RT-PCR 실험을 위한 RNA를 정량하는데 효과적입니다.
  • Linear range : 5-1000ng RNA
  • Dye Ex/Em : 650/670nm
  • Kit의 구성 

         - RNA Broad Range Dye, 200X

         - RNA Broad Range Buffer

         - RNA Dilution Buffer (can use as Standard 1 on Qubit®)

         - RNA Broad Range Standard, 100 ng/uL* (can use as Standard 2 on Qubit®)




Assay range
The assay is linear between 5 and 1000 ng of RNA per 200 uL assay well, which spans both Qubit® RNA HS and Qubit® BR ranges. It is ideal for use in quantifying RNA for sensitive applications such as Next-Gen Sequencing (NGS) or reverse transcription PCR (RT-PCR).





Linearity of AccuBlue® Broad Range RNA Quantitation assay between 5 and 1000 ng of RNA per well in microplate assay with excitation/emission at 630/670 nm. 




The AccuBlue® Broad Range RNA quantitation assay can detect single-stranded DNA (ssDNA), but with lower affinity than longer RNA. Dilution series of two types of ssDNA (M13mp18 linearized plasmid and salmon sperm DNA) were compared to the same concentrations of RNA Broad Range Standard (total Jurkat RNA). The fluorescence values of the ssDNA were lower overall; M13mp18 ssDNA was ~50% lower, and salmon sperm DNA was ~ 75% lower.





RNA Broad Range Standard, 100 ng/uL (#99850) 

  • Stand-alone total RNA standard
  • Mammalian cell total RNA in 1X Tris-EDTA
  • 100 ng/uL
  • 300 uL
  • Store at -20°C or below (can be stored at -80°C for long-term stability)


주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
AccuBlue® Broad Range RNA Quantitation Kit (1000 assays) 31073 1 kit BIOTIUM
AccuBlue® Broad Range RNA Quantitation Kit (200 assays) 31073-T 1 KT BIOTIUM
RNA Broad Range Standard, 100 ng/uL 99850 1 EA BIOTIUM
Maker
BIOTIUM
Cat.No.
31073
Product
AccuBlue® Broad Range RNA Quantitation Kit (1000 assays)
Size
1 kit
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
31073-T
Product
AccuBlue® Broad Range RNA Quantitation Kit (200 assays)
Size
1 KT
Qty
Data Sheet
MSDS
Maker
BIOTIUM
Cat.No.
99850
Product
RNA Broad Range Standard, 100 ng/uL
Size
1 EA
Qty
Data Sheet
MSDS

추천제품

관련제품

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
AccuBlue® NextGen dsDNA Quantitation Kit (1000 assays) 31060 1 kit BIOTIUM
AccuClear® Ultra High Sensitivity dsDNA Quantitation Kit with 8 DNA Standards (1000 assays) 31028 1 kit BIOTIUM
RNA Broad Range Standard, 100 ng/uL 99850 1 EA BIOTIUM
RNase-Free Calf Thymus DNA, 1 mg/mL (1 mL) 31065 1 ml BIOTIUM

자료

  • DNA & RNA Quantitation Kits

    DNA & RNA Quantitation Kits 이미지

    [BIOTIUM]

    DNA, RNA 측정 kit을 소개합니다.

웨비나/Video

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제품 문의

 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
Maker
Cat.No.
Product
Size
Qty
Data Sheet
MSDS

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