[Novus] INSTA-Blot Western Blot Membranes | 고마바이오텍

특징 

• INSTA-Blot은 이미 단백질이 로딩되어 제공되는 PVDF membrane입니다. 
• 단백질 추출, 정량, 전기영동, transfer 과정이 생략되므로, WB 절차를 손쉽게 진행할 수 있습니다. 
• 각 membrane에는 분자량 마커와 다양한 세포/조직의 변성된 단백질 용해물(lysates)이 로딩되어 있습니다.

INSTA-Blot을 이용한 Western Blot 진행과정

1. Blocking 
2. Primary antibody incubation (1차 항체 배양)
3. Washing 
4. Secondary antibody incubation (2차 항체 배양)
5. Washing 
6. WB development

제품 종류   

데이터

Western Blot: INSTA-Blot Human Tissues [NBP2-30113] - Human Tissues. Approximately 20 ug per lane of human tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker (Mark12); 2. Brain; 3. Heart; 4. Small Intestine; 5. Kidney; 6. Liver; 7. Lung; 8. Skeletal Muscle; 9. Stomach; 10. Spleen; 11. Ovary; 12. Testis

Western Blot: INSTA-Blot Human Tissues [NBP2-31378] - SDS-Page: INSTA-Blot Human Tissues [NBP2-31378] - Approximately 50 ug per lane of human tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker; 2. Brain; 3. Heart; 4. Small Intestine; 5. Kidney; 6. Liver; 7. Lung; 8. Skeletal Muscle; 9. Stomach; 10. Spleen; 11. Ovary; 12. Testis

SDS-Page: INSTA-Blot Colon Tissue OncoPair [NBP2-30129] - Samples on the blot are layed out in pairs of tumor and normal tissue lysate from the same patient. There are seven pairs of samples, representing seven different patients, on each OncoPair blot.

SDS-Page: INSTA-Blot Human Brain [NBP2-30119] - Approximately 30 ug per lane of tissue lysate is resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker; 2-12 human brain.