ExCellerate iPSC Expansion Medium | 고마바이오텍

특징 

• 미분화 iPSC의 탁월한 expansion과 유지에 사용됩니다. 
• Growth factor를 보충할 필요가 없는 all-in-one formulation을 제공합니다.
• 일관성 있는 결과를 제공합니다. 
• 다양한 matrice와 cell line에 사용됩니다. 
• 장기 배양에 이용 시, 세포 생존력, 유전자형 및 분화 잠재력이 안정하게 유지됩니다.  

데이터

Using ExCellerate iPSC Media better supports low density iPSC culture compared to other media suppliers.

Human iPSCs were plated at low density (3000 cells per well in a 6 well plate) and cultured until the cells reached approximately 70% confluence for 3 passages. Cells were harvested using Accutase and live cells were counted. All groups were then normalized to CCM036-GMP. Competitors showed slower and highly variable cell growth (Plot A) as well as lower viability (Plot B) compared to CCM036-GMP.

ExCellerate™ iPSC Expansion Medium maintain expression of stemness markers over long-term cultures (at least 24-passages).

Human iPSC expanded in CCM036-GMP express key stemness markers, Oct 3/4 and Nanog. SSEA-1 used as negative control.

Stable genotype over long-term culture.

Human iPSCs cultured in CCM036-GMP show characteristics healthy colony morphology including clearly defined edges, compact and tightly packed cells, along with normal karyotype after 26 passages.