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Carbohydrate Metabolism Assay Kit 이미지

Carbohydrate Metabolism Assay Kit

β-Hydroxybutyrate (Ketone Body), G6PDH, Lactate, Lactose, Starch 등 탄수화물 대사에 관련된 다양한 물질을 분석하는 assay kit 입니다.

β-Hydroxybutyrate (Ketone Body) Assay Kit (#MET-5083)  

 

  • 케톤 (Ketone)은 간과 신장에서 지방산 산화로 생성되는 수용성 분자입니다.
  • β-Hydroxybutyrate는 혈청에서 가장 많이 발생하며 측정된 모든 Ketone Body의 약 75 %를 차지합니다.
  • 높은 수준의 ketone bodies 는 케톤증이나 케톤 산증을 유발할 수 있습니다.
  • β-Hydroxybutyrate (Ketone Body) Assay Kit는 다양한 샘플에 존재하는 총 β-BH를 측정할 수 있습니다
  • NAD +가 NADH로 환원되는 효소 순환 반응에 기초합니다.
  • 96well 로 colorimetric 와 fluorometric 방법으로 각각 제공됩니다.
  • Detection sensitivity limit : 약 31 uM (colorimetric) or 2 uM (fluorometric)
  • serum, plasma, lysates, urine, cell lysates, tissue homogenates 샘플로 분석 가능합니다.

β-Hydroxybutyrate (Ketone Body) Assay Kit (#MET-5083)

 

 

 

Glucose-6-Phosphate Dehydrogenase (G6PDH) Activity Assay Kit (#MET-5081)

 

  • Glucose-6-Phosphate Dehydrogenase (G6PDH) 는 다른세포과정 뿐만 아니라 해당 과정에서도 중요한 효소입니다
  • simple colorimetric assay 로 96well로 편리하게 세포, 조직에서 G6PDH의 actibity를 정량할 수 있습니다
  • Detection sensitivity limit : 약 1 mUnit/mL
  • suspension and adherent cells, tissue homogenates 샘플로 분석 가능합니다.
  • G6PDH standard가 제공됩니다.

 

 

 

 

Lactate Assay Kits (#MET-5012, MET-5013)

  • Biological sample내 존재하는 L-lactate를 측정합니다.
  • Lactate는 lactate oxidase에 의해 산화되어 pyruvate와 hydrogen peroxide를 생성하고, hydrogen peroxide는 컬러나 형광법으로 측정됩니다. 
  • Lactate standard curve 대비 unknown sample의 lactate양을 측정합니다. 


Lactate Assay Kits (#MET-5012, MET-5013)


Lactose Assay Kit (#MET-5001)

  • Milk를 포함한 식품 및 biological sample내 존재하는 lactose를 측정합니다.
  • lactose는 lactase에 의해 glucose와 galactose로 분해되며 glucose는 glucose oxidase에 의해 산화되어 hydrogen oxide와 D-gluconic acid를 방출합니다. 
  • 이 과정에서 유리된 hydrogen peroxide를 측정함으로써 lactose의 양을 측정합니다. 

Lactose Assay Kit (#MET-5001)


Starch Assay Kits (#MET-5025, MET-5026)

  • Food sample에 존재하는 starch를 측정하기 위한 kit 입니다.
  • Starch는 amyloglucosidase에 의해 glucose monomer로 분해되며, glucose는 glucose oxidase에 의해 산화되어 D-gluconic acid와 hydrogen peroxide를 생성합니다. 
  • 이 hydrogen peroxidase는 kit에서 제공하는 probe와 반응하여 컬러 및 형광법으로 측정됩니다. 



사용 논문

MET-5083

Inokuchi, S. et al. (2021). Suppression of optineurin impairs the progression of hepatocellular carcinoma through regulating mitophagy. Cancer Med. doi: 10.1002/cam4.3519 (#MET-5082).

MET-5081  
    1. Siddiqui, S.H. et al. (2021). Altered relationship between gluconeogenesis and immunity in broilers exposed to heat stress for different durations. Poult Sci. doi: 10.1016/j.psj.2021.101274.
    2. Zhu, Z. et al. (2020). Itaconate regulates the glycolysis/pentose phosphate pathway transition to maintain boar sperm linear motility by regulating redox homeostasis. Free Radic Biol Med159:44-53. doi: 10.1016/j.freeradbiomed.2020.07.008.

MET-5012

MET-5013

 
    1. Stefan, J. et al. (2021). Differential and Overlapping Effects of Melatonin and Its Metabolites on Keratinocyte Function: Bioinformatics and Metabolic Analyses. Antioxidants10(4):618. doi: 10.3390/antiox10040618 (#MET-5012).
    2. Meléndez, D.M. et al. (2021). Correlation between L-Lactate Concentrations in Beef Cattle, Obtained Using a Hand-Held Lactate Analyzer and a Lactate Assay Colorimetric Kit. Animals (Basel)11(4):926. doi: 10.3390/ani11040926 (#MET-5012).
    3. Meléndez, D.M. et al. (2021). Effects of conditioning, source, and rest on indicators of stress in beef cattle transported by road. PLoS One16(1):e0244854. doi: 10.1371/journal.pone.0244854 (#MET-5012).
    4. Rattu, G. et al. (2020). Lactate detection sensors for food, clinical and biological applications: a review. Environ Chem Lett. doi: 10.1007/s10311-020-01106-6 (#MET-5012).
    5. Lomeli, M.J.M. et al. (2020). Use of artificial illumination to reduce Pacific halibut bycatch in a U.S. West Coast groundfish Bottom trawl. Fish Res. doi: 10.1016/j.fishres.2020.105737 (#MET-5012).
    6. Aldonza, M. B. D. et al. (2020). Prior acquired resistance to paclitaxel relays diverse EGFR-targeted therapy persistence mechanisms. Sci. Adv6(6): eaav7416. doi: 10.1126/sciadv.aav7416 (#MET-5012).
    7. Kaneko, Y. et al. (2020). Rhynchophylline promotes stem cell autonomous metabolic homeostasis. Cytotherapy. pii: S1465-3249(19)30925-9. doi: 10.1016/j.jcyt.2019.12.008 (#MET-5013).
    8. Gospe, S.M. et al. (2019). Photoreceptors in a Mouse Model of Leigh Syndrome are Capable of Normal Light-Evoked Signaling. J Biol Chem. pii: jbc.RA119.007945. doi: 10.1074/jbc.RA119.007945 (#MET-5012).
    9. Ogando, J. et al. (2019). PD-1 signaling affects cristae morphology and leads to mitochondrial dysfunction in human CD8+ T lymphocytes. J Immunother Cancer7(1):151. doi: 10.1186/s40425-019-0628-7 (#MET-5013).
    10. Guo, R. et al. (2018). Cardiomyocyte-specific disruption of Cathepsin K protects against doxorubicin-induced cardiotoxicity. Cell Death Dis9(6):692. doi: 10.1038/s41419-018-0727-2 (#MET-5012).
    11. Longchamp, A. et al. (2018). Amino Acid Restriction Triggers Angiogenesis via GCN2/ATF4 Regulation of VEGF and H2S Production. Cell. 173(1):117-129.e14. doi: 10.1016/j.cell.2018.03.001 (#MET-5012).
MET-5001

Li, Y.  et al. (2021). Nrf2-ARE Signaling Partially Attenuates Lipopolysaccharide-Induced Mammary Lesions via Regulation of Oxidative and Organelle Stresses but Not Inflammatory Response in Mice. Oxid Med Cell Longev.  doi: 10.1155/2021/8821833.

MET-5025

MET-5026

Murakami, R. et al. (2021). A new galling insect model enhances photosynthetic activity in an obligate holoparasitic plant. Sci Rep11(1):13013. doi: 10.1038/s41598-021-92417-3 (#MET-5025).


주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
2-Phosphoglycerate Assay Kit MET-5167 100 assays CELL BIOLABS
Adenosine Monophosphate Assay Kit MET-5160 100 assays CELL BIOLABS
ADP Assay Kit MET-5164 100 assays CELL BIOLABS
ATP Assay Kit MET-5163 100 assays CELL BIOLABS
Beta-Hydroxybutyrate (Ketone Body) Assay Kit (Colorimetric) MET-5082 100 assays CELL BIOLABS
Beta-Hydroxybutyrate (Ketone Body) Assay Kit (Fluorometric) MET-5083 100 assays CELL BIOLABS
Branched Chain Amino Acid Assay Kit MET-5056 192 assays CELL BIOLABS
Carbohydrate (total) Assay Kit ARG82228 100 assay ARIGO
Glucose Assay Kit (Colorimetric) STA-680 500 assays CELL BIOLABS
Glucose Assay Kit (Fluorometric) STA-681 500 assays CELL BIOLABS
Glucose-6-Phosphate Dehydrogenase (G6PDH) Activity Assay Kit MET-5081 100 assays CELL BIOLABS
Glycogen Assay Kit (Colorimetric) MET-5022 100 assays CELL BIOLABS
Glycogen Assay Kit (Fluorometric) MET-5023 100 assays CELL BIOLABS
Glycogen Branching Enzyme Assay Kit ARG82031 96 wells ARIGO
Ketone Body Assay Kit (Colorimetric) ARG82173 200 tests ARIGO
Lactate (D-Lactate) Assay Kit ARG82836 100tests ARIGO
Lactate Assay Kit (Colorimetric) ARG82235 100 assay ARIGO
Lactate Assay Kit (Colorimetric) MET-5012 100 assays CELL BIOLABS
Lactate Assay Kit (Fluorometric) ARG82236 100 assay ARIGO
Lactate Assay Kit (Fluorometric) MET-5013 100 assays CELL BIOLABS
Lactose Assay Kit MET-5001 100 assays CELL BIOLABS
Malate Assay Kit (Colorimetric) MET-5119 200 assays CELL BIOLABS
Malate Assay Kit (Colorimetric) ARG82178 100 tests ARIGO
Malate Assay Kit (Fluorometric) MET-5120 100 assays CELL BIOLABS
Maltose Assay Kit (Colorimetric) ARG82180 100 tests ARIGO
Phosphoenolpyruvate Assay Kit Phosphoenolpyruvate Assay Kit 100 assays CELL BIOLABS
Pyrophosphate Assay Kit Pyrophosphate Assay Kit 100 assays CELL BIOLABS
Pyruvate Assay Kit MET-5029 100 assays CELL BIOLABS
Pyruvate Assay Kit (Colorimetric) MET-5125 100 assays CELL BIOLABS
Starch Assay Kit ARG82019 96 wells ARIGO
Starch Assay Kit (Colorimetric) MET-5025 100 assays CELL BIOLABS
Starch Assay Kit (Fluorometric) MET-5026 100 assays CELL BIOLABS
Total Carbohydrate Assay Kit STA-682 100 assays CELL BIOLABS
Total Thiol Assay Kit (Colorimetric) MET-5053 192 assays CELL BIOLABS
전체보기
Maker
CELL BIOLABS
Cat.No.
MET-5167
Product
2-Phosphoglycerate Assay Kit
Size
100 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
MET-5160
Product
Adenosine Monophosphate Assay Kit
Size
100 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
MET-5164
Product
ADP Assay Kit
Size
100 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
MET-5163
Product
ATP Assay Kit
Size
100 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
MET-5082
Product
Beta-Hydroxybutyrate (Ketone Body) Assay Kit (Colorimetric)
Size
100 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
MET-5083
Product
Beta-Hydroxybutyrate (Ketone Body) Assay Kit (Fluorometric)
Size
100 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
MET-5056
Product
Branched Chain Amino Acid Assay Kit
Size
192 assays
Qty
Data Sheet
MSDS
Maker
ARIGO
Cat.No.
ARG82228
Product
Carbohydrate (total) Assay Kit
Size
100 assay
Qty
Data Sheet
Maker
CELL BIOLABS
Cat.No.
STA-680
Product
Glucose Assay Kit (Colorimetric)
Size
500 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-681
Product
Glucose Assay Kit (Fluorometric)
Size
500 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
MET-5081
Product
Glucose-6-Phosphate Dehydrogenase (G6PDH) Activity Assay Kit
Size
100 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
MET-5022
Product
Glycogen Assay Kit (Colorimetric)
Size
100 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
MET-5023
Product
Glycogen Assay Kit (Fluorometric)
Size
100 assays
Qty
Data Sheet
MSDS
Maker
ARIGO
Cat.No.
ARG82031
Product
Glycogen Branching Enzyme Assay Kit
Size
96 wells
Qty
Data Sheet
Maker
ARIGO
Cat.No.
ARG82173
Product
Ketone Body Assay Kit (Colorimetric)
Size
200 tests
Qty
Data Sheet
Maker
ARIGO
Cat.No.
ARG82836
Product
Lactate (D-Lactate) Assay Kit
Size
100tests
Qty
Data Sheet
Maker
ARIGO
Cat.No.
ARG82235
Product
Lactate Assay Kit (Colorimetric)
Size
100 assay
Qty
Data Sheet
Maker
CELL BIOLABS
Cat.No.
MET-5012
Product
Lactate Assay Kit (Colorimetric)
Size
100 assays
Qty
Data Sheet
MSDS
Maker
ARIGO
Cat.No.
ARG82236
Product
Lactate Assay Kit (Fluorometric)
Size
100 assay
Qty
Data Sheet
Maker
CELL BIOLABS
Cat.No.
MET-5013
Product
Lactate Assay Kit (Fluorometric)
Size
100 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
MET-5001
Product
Lactose Assay Kit
Size
100 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
MET-5119
Product
Malate Assay Kit (Colorimetric)
Size
200 assays
Qty
Data Sheet
MSDS
Maker
ARIGO
Cat.No.
ARG82178
Product
Malate Assay Kit (Colorimetric)
Size
100 tests
Qty
Data Sheet
Maker
CELL BIOLABS
Cat.No.
MET-5120
Product
Malate Assay Kit (Fluorometric)
Size
100 assays
Qty
Data Sheet
MSDS
Maker
ARIGO
Cat.No.
ARG82180
Product
Maltose Assay Kit (Colorimetric)
Size
100 tests
Qty
Data Sheet
Maker
CELL BIOLABS
Cat.No.
Phosphoenolpyruvate Assay Kit
Product
Phosphoenolpyruvate Assay Kit
Size
100 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
Pyrophosphate Assay Kit
Product
Pyrophosphate Assay Kit
Size
100 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
MET-5029
Product
Pyruvate Assay Kit
Size
100 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
MET-5125
Product
Pyruvate Assay Kit (Colorimetric)
Size
100 assays
Qty
Data Sheet
MSDS
Maker
ARIGO
Cat.No.
ARG82019
Product
Starch Assay Kit
Size
96 wells
Qty
Data Sheet
Maker
CELL BIOLABS
Cat.No.
MET-5025
Product
Starch Assay Kit (Colorimetric)
Size
100 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
MET-5026
Product
Starch Assay Kit (Fluorometric)
Size
100 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
STA-682
Product
Total Carbohydrate Assay Kit
Size
100 assays
Qty
Data Sheet
MSDS
Maker
CELL BIOLABS
Cat.No.
MET-5053
Product
Total Thiol Assay Kit (Colorimetric)
Size
192 assays
Qty
Data Sheet
MSDS
전체보기

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0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
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