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피부 연구를 위한 ELISA Kit 이미지

피부 연구를 위한 ELISA Kit

FGF7, Elafin, CD10, CALML5 등 피부 연구를 위한 ELISA Kit을 소개합니다.

배경

 

Fibroblast growth factor 7 (FGF 7)은 상피 세포 특이적 성장 인자입니다. Mouse 및 Rat homolog에 대한 연구에 의해 FGF 7 은 epidermis의 형태 형성, 상처의 reepithelialization, 모발 발달 및 초기 폐 기관 형성에 관여하는 것으로 보여집니다.

 

Elafin은 피부의 호중구 및 췌장 elastase에 특이적인 억제제이며, elastase에 의해 매개되는 조직 단백질 분해를 예방할 수 있습니다. 이 유전자의 발현은 박테리아 LPS와 사이토카인에 의해 상승 조절됩니다. 

 

Type 1 콜라겐은 대부분의 connective tissue에 존재하는 fobril-forming 콜라겐으로, 뼈, 각막, 진피 및 힘줄에 풍부합니다. 이 유전자의 돌연변이는 골 형성 부전 유형 I-IV, Ehlers-Danlos 증후군 VIIA 유형, Ehlers-Danlos 증후군 Classical type, Caffey 질병 및 idiopathic osteoporosis과 관련이 있습니다. 또한, dermatofibrosarcoma protuberans와 같은 특정 피부암과 관련이 있습니다. 

 

CD 10 은 UV에 의해 유도되는 피부 preelastic and elastic fiber와 관련된 elastase의 활성을 보입니다. 

 

CALML 5는 epidermis에서 발현되고 칼슘 결합 단백질(calcium binding proteins)의 calmodulin family와 관련된 새로운 칼슘 결합 단백질을 암호화합니다. 재조합 단백질에 대한 기능적 연구를 통해 칼슘에 결합하고 이 때 구조적 변화를 겪는다는 것이 밝혀졌습니다. Reconstructed epidermis에서만 풍부하게 검출되며 분화된 각질 세포에 제한됩니다. 또한 각질 세포의 최종 분화에서 핵심 효소인 것으로 밝혀진 transglutaminase 3과 결합할 수 있습니다. 

 

FABP5는 표피 세포에서 발견되는 지방산 결합 단백질을 암호화하며, 건선 조직(psoriasis tissue)에서 상향 조절됩니다. 또한 FABP는 지방산 흡수, 수송 및 대사에 역할을 하며,  이 유전자의 다형성은 제2형 당뇨병과 연관이 있습니다.

 

 

 

종류

 

Human KGF ELISA Kit (#ARG81263)
Product Description ARG81263 Human KGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human KGF in serum, plasma or cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name KGF
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15 pg/ml
Sample Type Serum, plasma or cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Alternate Names FGF-7; Fibroblast growth factor 7; Heparin-binding growth factor 7; KGF; Keratinocyte growth factor; HBGF-7
   
Human CD10 ELISA Kit (#ARG81762)
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD10
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 62.5 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 125 - 8000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.1%; Inter-Assay CV: 7.4%
Alternate Names Enkephalinase; Neutral endopeptidase; Neutral endopeptidase 24.11; Common acute lymphocytic leukemia antigen; Neprilysin; CD antigen CD10; CALLA; EC 3.4.24.11; CD10; Skin fibroblast elastase; SFE; Atriopeptidase; NEP
   
Human Elafin ELISA Kit (#ARG81504)
Product Description ARG81504 Human Elafin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Elafin in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Elafin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.7%
Alternate Names Protease inhibitor WAP3; cementoin; WFDC14; Skin-derived antileukoproteinase; WAP four-disulfide core domain protein 14; SKALP; Elafin; Elastase-specific inhibitor; WAP3; Peptidase inhibitor 3; ESI; PI-3
   
Human CALML5 ELISA Kit (#ARG82604)
Product Description ARG82604 Human CALML5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CALML5 in serum, plasma and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity This kit could assay both natural and recombinant Human CALML5.

No significant cross-reactivity or interference was observed in the following samples:
Human: CALML3, GM-CSF, IFN gamma, IL1 beta, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL17A, IL18, IL21, IL22, IL23, MCP1, MIP1 beta, SCF, TGF beta 1, TNF alpha and VEGF.
Mouse: GM-CSF, IFN gamma, IL1 beta, IL2, IL4, IL6, IL10, IL17A and TNF alpha.
Rat: IFN gamma, IL1 beta, IL4, IL6, IL10 and TNF alpha.
Target Name CALML5
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 23.5 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 46.9 - 3000 pg/ml
Sample Volume 20 µl
Precision Intra-Assay CV: 4.2%
Inter-Assay CV: 2.0%
Alternate Names CLSP; Calmodulin-like skin protein; Calmodulin-like protein 5
   
Human Pro-Collagen I alpha 1 ELISA Kit (#ARG83041)
Product Description ARG83041 Human Pro-Collagen I alpha 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Pro-Collagen I alpha 1 in serum, plasma (heparin), ascites, urine and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity Not cross-reacts with:
Human BMP2, BMP4, TIE2, Collagen II, Collagen III alpha1.
Mouse BCMA, VEGF, TNF alpha, Collagen II.
Rat Collagen II
Target Name COL1A1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Protein Full Name Collagen alpha-1(I) chain
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin), ascites, urine and cell culture supernatants.
Standard Range 62.5-4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: < 10%
Inter-Assay CV: < 10%
Alternate Names COL1A1, Collagen Type I Alpha 1 Chain, OI4, Collagen, Type I, Alpha 1, Collagen Alpha-1(I) Chain, Alpha-1 Type I Collagen, Collagen Of Skin, Tendon And Bone, Alpha-1 Chain, Collagen Alpha-1(I) Chain Preproprotein, Type I Procollagen Alpha 1 Chain, Collagen Alpha 1 Chain Type I, Pro-Alpha-1 Collagen Type , Alpha1(I) Procollagen, Type I Proalpha, EDSARTH1, CAFYD, EDSC, OI1, OI2, OI3
Human FABP5 ELISA Kit (#ARG81389)
Product Description ARG81389 Human FABP5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human FABP5 in serum, plasma and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity Cross-Reactivity: Not react with FABP1, FABP2, FABP3, FABP4, FABP6, FABP7, FABP8, and FABP9.
Target Name FABP5
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.6 ng/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 0.75 - 24 ng/ml
Sample Volume 50 µl
Alternate Names PA-FABP; Epidermal-type fatty acid-binding protein; KFABP; EFABP; E-FABP; Psoriasis-associated fatty acid-binding protein homolog; Fatty acid-binding protein, epidermal; Fatty acid-binding protein 5; PAFABP
Mouse CD10 ELISA Kit (#ARG81763)
Product Description ARG81763 Mouse CD10 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD10 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD10
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 46.85 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 93.7 - 6000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.1%; Inter-Assay CV: 6.0%
Alternate Names Enkephalinase; Neutral endopeptidase; Neutral endopeptidase 24.11; Common acute lymphocytic leukemia antigen; Neprilysin; CD antigen CD10; CALLA; EC 3.4.24.11; CD10; Skin fibroblast elastase; SFE; Atriopeptidase; NEP
Mouse CCL27 / CTACK ELISA Kit (#ARG81709)
Product Description ARG81709 Mouse CCL27 / CTACK ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CCL27 / CTACK in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CCL27 / CTACK
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.6%; Inter-Assay CV: 6.8%
Alternate Names ILC; SCYA27; CTACK; Cutaneous T-cell-attracting chemokine; CTAK; PESKY; ESkine; Skinkine; ESKINE; Small-inducible cytokine A27; IL-11 R-alpha-locus chemokine; C-C motif chemokine 27; ALP; CC chemokine ILC
Human FABP5 ELISA Kit (#ARG81389)
Product Description ARG81389 Human FABP5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human FABP5 in serum, plasma and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity Cross-Reactivity: Not react with FABP1, FABP2, FABP3, FABP4, FABP6, FABP7, FABP8, and FABP9.
Target Name FABP5
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.6 ng/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 0.75 - 24 ng/ml
Sample Volume 50 µl
Alternate Names PA-FABP; Epidermal-type fatty acid-binding protein; KFABP; EFABP; E-FABP; Psoriasis-associated fatty acid-binding protein homolog; Fatty acid-binding protein, epidermal; Fatty acid-binding protein 5; PAFABP



 

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
Human CALML5 ELISA Kit ARG82604 96 wells ARIGO
Human Elafin ELISA Kit ARG81504 96 wells ARIGO
Human FABP5 ELISA Kit ARG81389 96 wells ARIGO
Human KGF ELISA Kit ARG81263 96 wells ARIGO
Human Pro-Collagen I alpha 1 ELISA Kit ARG83041 96wells ARIGO
Maker
ARIGO
Cat.No.
ARG82604
Product
Human CALML5 ELISA Kit
Size
96 wells
Qty
Data Sheet
Maker
ARIGO
Cat.No.
ARG81504
Product
Human Elafin ELISA Kit
Size
96 wells
Qty
Data Sheet
Maker
ARIGO
Cat.No.
ARG81389
Product
Human FABP5 ELISA Kit
Size
96 wells
Qty
Data Sheet
Maker
ARIGO
Cat.No.
ARG81263
Product
Human KGF ELISA Kit
Size
96 wells
Qty
Data Sheet
Maker
ARIGO
Cat.No.
ARG83041
Product
Human Pro-Collagen I alpha 1 ELISA Kit
Size
96wells
Qty
Data Sheet

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0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
Maker
Cat.No.
Product
Size
Qty
Data Sheet
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