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Rho-associated kinase (ROCK) Assay Kits 이미지

Rho-associated kinase (ROCK) Assay Kits

Rho-associated kinase (ROCK)에 의해 MYPT1의 Thr696 부분에 인산화가 되었는지를 확인함으로써 ROCK의 활성을 측정하는 kit 입니다. ELISA방식과 Western Blot 방식으로 제공됩니다.

96-well ROCK Activity Assay Kit (#STA-416) 


  • 재조합 MYPT1으로 코팅된 microplate에 ROCK sample (예, purified kinase, cell/tissue lysate등)을 반응시킨 후 anti-phospho-MYT1(Thr696) 항체로 phosphorylated MYPT1을 측정합니다. 
  • 방사성동위원소를 사용하지 않습니다.
  • Detection sensitivity limit : 200 pg of active ROCK-II 

ROCK-II_Activity_Assay
ROCK-II Activity Assay. Left: Active ROCK-II was incubated for 60 minutes at 30°C.
Right: 2.5 ng of active ROCK-II was incubated at 30°C for times as shown. Phosphorylation of
MYPT1 substrate was detected by anti-phospho-MYPT1 (Thr696) antibody as described in Assay Protocol.



Rho Kinase (ROCK) Activity Immunoblot Kit (#STA-415)

  • MYPT1을 ROCK 샘플과 반응시킨 후 생성된 phosphorylated MYPT1을 anti-phospho-MYPT1(Thr696) 항체로 Western blot 상에서 확인합니다. 
  • 방사성동위원소를 사용하지 않습니다.
  • Active ROCK-II가 positive control로 제공됩니다. 

ROCK-II_Activity_Immunoblot_Assay

ROCK-II Activity Immunoblot Assay. 25 μL of 1X Kinase Buffer containing 10 ng of
active ROCK-II was incubated with 50 μL of 1X Kinase Buffer containing 0.2 mM ATP and 500 ng of
recombinant MYPT1 for 30 minutes at 30°C. Kinase reaction was stopped by adding 25 μL of 4X
SDS-PAGE Sample Buffer. Lane 1, 3, 5, 7: Without kinase (negative control); Lane 2, 4, 6, 8: with
kinase. 200 ng (Lane 1 and 2), 100 ng (Lane 3 and 4), 50 ng (Lane 5 and 6) or 25 ng (Lane 7 and 8) of
recombinant MYPT1 substrate were loaded onto SDS-PAGE. Phosphorylation of MYPT1 substrate
was detected by anti-phospho-MYPT1 (Thr696) antibody as described in Assay Protocol.


주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
96-Well ROCK Activity Assay Kit STA-416 96 assays CELL BIOLABS
96-Well ROCK Activity Assay Kit STA-416-5 5x96 assays CELL BIOLABS
ROCK Activity Immunoblot Kit STA-415 20 assays CELL BIOLABS
Maker
CELL BIOLABS
Cat.No.
STA-416
Product
96-Well ROCK Activity Assay Kit
Size
96 assays
Qty
Data Sheet
Maker
CELL BIOLABS
Cat.No.
STA-416-5
Product
96-Well ROCK Activity Assay Kit
Size
5x96 assays
Qty
Data Sheet
Maker
CELL BIOLABS
Cat.No.
STA-415
Product
ROCK Activity Immunoblot Kit
Size
20 assays
Qty
Data Sheet

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