CRISPR Mutation Detection kit | sgRNA Screening & Synthesis Kit | 고마바이오텍

GenCRISPR Mutation Detection Kit (#L00688) 

• TALEN, CRISPR, ZFN과 같은 유전자 편집프로그램이 도입된 세포에서 추출된 genomic DNA의 site-specific cleavage를 확인하기 위한 킷트 입니다.
• 구성품 :
• High-Fidelity DNA polymerase
• 5X PCR Reaction Buffer
• GenCrispr T7 Endonuclease I (Cat.No.Z03396)
• 10X GenCrispr T7 Endonuclease I Reaction buffer
• Control Template DNA
• Control Primer Mix
• Protease K
• dNTP

• GenCRISPR sgRNA Screening Kit는 세포 형질 도입 전에 single guide RNA (sgRNA)의 성능을 평가하기 위해 이용됩니다. 
• 구성품 : 
• GenCrispr Cas9 Nuclease
• 10X Reaction Buffer
• Positive Control sgRNA
• Positive Control Substrate
• RNase-free water

gRNA efficiency screening using GenCRISPR sgRNA screening kit.

Twelve unique synthesized gRNAs were incubated with Cas9 nuclease for 1 h at 37°C according to the instructions in theGenCRISPR sgRNA screening kit manual. 

Upon separating the reactions by agarose gel electrophoresis, only gRNA9 was observed to induce cleavage.

GenCRISPR sgRNA Synthesis Kit (#L00694)

• GenCRISPR sgRNA synthesis kit는 gRNA를 합성하기 위한 kit 입니다. 
• T7 promoter를 포함한 gRNA DNA template를 생성하고, gRNA를 합성합니다. 
• 구성품 :

• 10 μM TracrRNA fragment 

• 10 μM T7 Primer Mix 
• 10 X PCR Reaction buffer 
• High-Fidelity polymerase 
• 10 μM dNTP mixture 
• Control gRNA forward and reverse primers (10 μM )
• 50 mM DTT 
  
• 10 X Transcription Reaction buffer 
• 10 mM NTP mix 
• GenCrispr T7 RNA polymerase 
• RNase inhibitor 
• Nuclease-free water