CRISPR Cas9 Nuclease

제품 상세

유전자 편집 성능이 입증된 Cas9, eSpCas9, Cas12a, Cas13a Nuclease 및 Cas9, FnCpf1, SpCas9 Antibody를 소개합니다. GMP 등급으로도 제공합니다.

프린트 제품 문의

GenCRISPR™ Nuclease

1) Cas9

Cas9 Nuclease는 E. coli에서 정제된 재조합 Streptococcus pyogenes Cas9 (wt) 단백질로, dsDNA에서 부위 특이적인 이중가닥 파손을 유도하는 강력한 유전자 편집 도구입니다.
Cas9 단백질은 CRISPR/Cas9 시스템의 guide RNA와 매우 안정적인 RNP (ribonucleoprotein) complex를 형성합니다.
RNP complex는 gRNA를 genomic DNA sequence와 매칭시켜 타겟 사이트를 인식하고 NGG PAM으로부터 3개 염기 내에서 DNA break를 유도합니다.

2) Cas12a

Cas9 시스템과 달리 CRISPR/Cpf1 시스템은 크기가 더 작고 추가 trans-activating crRNA (tracrRNA)가 필요하지 않습니다.
Cas9 시스템이 G-rich PAM을 필요로 하는 반면, Cas12a 시스템은 짧은 T-rich PAM에 의해 표적 DNA를 절단합니다.
4 또는 5-nt 5' overhang이 있는 엇갈린 (staggered) DNA 이중 가닥을 파손시킵니다.
BV3L6 Cas12a (cpf1) nuclease는 E.coli에서 발현 및 정제되며 C-말단에 핵 국소화 서열 (nuclear localization sequence, NLS)를 포함하고 6x His-tag을 포함합니다.

3) Cas13a

GenCRISPR Cas13a (C2c2) Nuclease는 RNA유도, RNA표적화 CRISPR 효소로, VI 타입 CRISPR 제품군에 속합니다.
Cas13a는 두가지 RNase 활성을 나타냅니다. (cis-sequence specific RNA guided cleavage, non-specific trans-ribonuclease activity)
Cas13a 단백질은 높은 특이성이 입증된 단일 RNA 분자를 검출하는데 적용됩니다.

	GenCRISPR™ Nucleases
	Cas9 Nuclease	Cas12a Nuclease	Cas13a Nuclease
Targeted Nucleic Acid	ds DNA	ds or ss DNA	ss RNA
Size of the protein	~1,000 – 1,600 AA	~1,300 AA	~1,400 AA
Guide	tracrRNA + crRNA	crRNA	crRNA
Type of cut	Double-strand, blunt cut	Double-strand, staggered cut	ss RNA
Cleavage	Cis-cleavage	Cis-cleavage / Trans-cleavage	Cis-cleavage / Trans-cleavage
Targeting restriction	GC-rich sequences	AT-rich sequences	-
PAM/PFS	5' - NGG - 3'	5′-TTTV-3′ upstream	A or U
GenScript variants	eSp, eGFP, Nickase, WT	WT	WT
Compliance	Research / GMP	RUO	RUO

GMP grade GenCRISPR™ Nuclease

ISO 13485와 GMP 품질경영시스템 기준에 따라 제작된 CRISPR gene editing용 wide type & mutant type Cas9 enzyme
Regulatory Documentation 제공
세포, 유전자, 조직을 이용한 치료제 개발시 보조 물질로 사용될 수 있음.
- USP <1043>. Ancillary materials for Cell, Gene and Tissue-engineered products.
- ICH Q7 Good Manufacturing Practice Guide for Active Pharmaceutical Ingredients
- NMPA: Technical guidelines for pharmaceutical research and evaluation of immune cell therapy products
- Guideline and procedure specified in Chinese Pharmacopeia.

1) GenCRISPR™ Ultra NLS-Cas9-GMP (#Z03623-GMP)

Wild type recombinant Cas 9 nuclease
높은 편집 효율을 필요로 하는 대부분의 CRISPR gene editing에 사용
Appearance : Clear, colorless, liquid
Purity : ≥ 95% as analyzed by SDS-PAGE, ≥ 95% as analyzed by SEC-HPLC
Concentration by A280 : 10 mg/ml ± 1 mg/ml
Bioactivity (in vitro) : ≥ 90%
Residual DNase : ≤ 10 ng/mg
Residual RNase : ≤ 1 ng/mg
Endotoxin Level : < 10 EU/mg
Residual HCP : ≤ 10 ng/mg
Residual HCD : ≤ 1 ng/mg
Mycoplasma : < LOD
Sterility : Sterile

2) GenCRISPR™ Ultra eSpCas9-2NLS-GMP (#Z03624-GMP)

Mutant type recombinant enhanced specificity Cas 9 nuclease
오프타겟을 낮추고 타겟에 대한 견고한 cleavage 효과를 필요로 하는 CRISPR gene editing에 사용
Appearance : Clear, colorless, liquid
Purity : ≥ 95% as analyzed by SDS-PAGE, ≥ 95% as analyzed by SEC-HPLC
Concentration by A280: 10 mg/ml ± 1 mg/ml
Bioactivity (in vitro): ≥ 85%
Residual DNase: ≤ 10 ng/mg
Residual RNase: ≤ 1 ng/mg
Endotoxin Level: < 10 EU/mg
Residual HCP : ≤ 10 ng/mg
Residual HCD : ≤ 1 ng/mg
Mycoplasma : < LOD
Sterility : Sterile

종류

Category	Cat. No.	Product
SpCas9 Nuclease (wild-type)	Z03386	GenCrispr Cas9 Nuclease
	Z03385	GenCrispr Cas9-C-NLS Nuclease
	Z03388/Z03468	GenCrispr Cas9-N-NLS Nuclease
	Z03389/Z03469	GenCrispr NLS-Cas9-NLS Nuclease
	Z03701	GenCRISPR™ Cas9 v1.1
	Z03702	GenCRISPR™ Cas9 v1.2
	Z03623-GMP	GenCRISPR™ Ultra NLS-Cas9-GMP
	Z03621	GenCRISPR™ Ultra NLS-Cas9-Research
	Z03623	GenCRISPR™ Ultra NLS-Cas9-basic GMP
eSpCas9 Nuclease (mutant)	Z03470	GenCrispr eSpCas9-N-NLS Nuclease
	Z03624	GenCRISPR™ Ultra eSpCas9-2NLS-basic GMP
	Z03624-GMP	GenCRISPR™ Ultra eSpCas9-2NLS-GMP
	Z03622	GenCRISPR™ Ultra eSpCas9-2NLS-Research
	Z03692	GenCRISPR™ Ultra eSpCas9-N-NLS Research, tag-free
Cas9-eGFP Nuclease	Z03393/Z03467	GenCrispr NLS-Cas9-EGFP Nuclease
SaCas9 Nuclease	Z03699	GenCRISPR™ SaCas9 2NLS Nuclease
Cas9 Nickase D10A	Z03390	GenCrispr NLS-Cas9-D10A Nickase
Cas12a Nuclease	Z03502	GenCRISPR™ Cas12a (Cpf1) Nuclease
Cas12a Nuclease	Z03753	GenCRISPR™ LbCas12a Nuclease
Cas13a Nuclease	Z03486	GenCRISPR™ Cas13a (C2c2) Nuclease
Cas13a Nuclease	Z03742	GenCRISPR™ LbuCas13a Nuclease

주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
Product	Cat.No.	Size	Maker
GenCRISPR™ Cas12a (Cpf1) Nuclease	Z03502-100	100ug	GENSCRIPT
GenCRISPR™ Cas12a (Cpf1) Nuclease	Z03502-500	500ug	GENSCRIPT
GenCRISPR™ Cas12a (Cpf1) Nuclease	Z03502-1	1mg	GENSCRIPT
GenCRISPR™ Cas12a (Cpf1) Nuclease	Z03502-50	50ug	GENSCRIPT
GenCRISPR™ Cas13a (C2c2) Nuclease	Z03486-100	100ug	GENSCRIPT
GenCRISPR™ Cas13a (C2c2) Nuclease	Z03486-1	1mg	GENSCRIPT
GenCRISPR™ Cas13a (C2c2) Nuclease	Z03486-500	500ug	GENSCRIPT
GenCRISPR™ Cas9 Antibody, pAb, Rabbit	A01885-100	100ul	GENSCRIPT
GenCRISPR™ Cas9 Antibody, pAb, Rabbit	A01885-40	40ul	GENSCRIPT
GenCRISPR™ Cas9 Nuclease	Z03386-50	50ug (5×10ug)(0.2mg/ml)	GENSCRIPT
GenCRISPR™ Cas9 Nuclease	Z03386-10	10ug (0.2mg/ml)	GENSCRIPT
GenCRISPR™ Cas9-C-NLS Nuclease	Z03385-100	100ug (4mg/ml)	GENSCRIPT
GenCRISPR™ Cas9-C-NLS Nuclease	Z03385-50	50ug (1mg/ml)	GENSCRIPT
GenCRISPR™ Cas9-N-NLS Nuclease	Z03468-1	1mg (10mg/ml)	GENSCRIPT
GenCRISPR™ Cas9-N-NLS Nuclease	Z03388-50	50ug (1mg/ml)	GENSCRIPT
GenCRISPR™ Cas9-N-NLS Nuclease	Z03468-500	500ug (10mg/ml)	GENSCRIPT
GenCRISPR™ Cas9-N-NLS Nuclease	Z03388-100	100ug (4mg/ml)	GENSCRIPT
GenCRISPR™ Cas9-N-NLS Nuclease	Z03468-100	100ug (10mg/ml)	GENSCRIPT
GenCRISPR™ eSpCas9-N-NLS Nuclease	Z03470-300	300ug (4mg/ml)	GENSCRIPT
GenCRISPR™ eSpCas9-N-NLS Nuclease	Z03470-100	100ug (4mg/ml)	GENSCRIPT
GenCRISPR™ FnCpf1 Antibody (14H3), mAb, Mouse	A01958-40	40ug	GENSCRIPT
GenCRISPR™ FnCpf1 Antibody (9H6), mAb, Mouse	A01957-40	40ug	GENSCRIPT
GenCRISPR™ NLS-Cas9-D10A Nickase	Z03390-50	50ug (1mg/ml)	GENSCRIPT
GenCRISPR™ NLS-Cas9-D10A Nickase	Z03390-100	100ug (4mg/ml)	GENSCRIPT
GenCRISPR™ NLS-Cas9-D10A Nickase	Z03390-10	10ug (1mg/ml)	GENSCRIPT
GenCRISPR™ NLS-Cas9-EGFP Nuclease	Z03467-1	1mg(10mg/ml)	GENSCRIPT
GenCRISPR™ NLS-Cas9-EGFP Nuclease	Z03393-50	50ug (1mg/ml)	GENSCRIPT
GenCRISPR™ NLS-Cas9-EGFP Nuclease	Z03467-500	500ug(10mg/ml)	GENSCRIPT
GenCRISPR™ NLS-Cas9-EGFP Nuclease	Z03393-100	100ug (3mg/ml)	GENSCRIPT
GenCRISPR™ NLS-Cas9-EGFP Nuclease	Z03467-100	100ug(10mg/ml)	GENSCRIPT
GenCRISPR™ NLS-Cas9-NLS Nuclease	Z03389-50	50ug (1mg/ml)	GENSCRIPT
GenCRISPR™ NLS-Cas9-NLS Nuclease	Z03469-100	100ug (10mg/ml)	GENSCRIPT
GenCRISPR™ NLS-Cas9-NLS Nuclease	Z03389-100	100ug (4mg/ml)	GENSCRIPT
GenCRISPR™ NLS-Cas9-NLS Nuclease	Z03469-1	1mg (10mg/ml)	GENSCRIPT
GenCRISPR™ NLS-Cas9-NLS Nuclease	Z03469-500	500ug (10mg/ml)	GENSCRIPT
GenCRISPR™ SaCas9 2NLS Nuclease	Z03699-0.1	0.1mg	GENSCRIPT
GenCRISPR™ SaCas9 2NLS Nuclease	Z03699-1	1mg	GENSCRIPT
GenCRISPR™ SaCas9 2NLS Nuclease	Z03699-0.5	0.5mg	GENSCRIPT
GenCRISPR™ SpCas9 Antibody (14B6), mAb, Mouse	A01936-40	40ug	GENSCRIPT
GenCRISPR™ SpCas9 Antibody (4A1), mAb, Mouse	A01935-40	40ug	GENSCRIPT
GenCRISPR™ T7 Endonuclease I	Z03396-250	250U (10000 U/ml)	GENSCRIPT
GenCRISPR™ T7 Endonuclease I	Z03396-1250	1250U (10000 U/ml)	GENSCRIPT
GenCRISPR™ Ultra eSpCas9-2NLS-GMP	Z03624-GMP-5	5mg	GENSCRIPT
GenCRISPR™ Ultra eSpCas9-2NLS-GMP	Z03624-GMP-2.5	2.5mg	GENSCRIPT
GenCRISPR™ Ultra NLS-Cas9-GMP	Z03623-GMP-2.5	2.5mg	GENSCRIPT
GenCRISPR™ Ultra NLS-Cas9-GMP	Z03623-GMP-5	5mg	GENSCRIPT
GenCRISPR™ Ultra NLS-Cas9-Research	Z03621-0.5	0.5mg	GENSCRIPT
GenCRISPR™ Ultra NLS-Cas9-Research	Z03621-5	5mg	GENSCRIPT
GenCRISPR™ Ultra NLS-Cas9-Research	Z03621-1	1mg	GENSCRIPT
GenCRISPR™ SaCas9 Antibody (11C12), mAb, Mouse	A01951-40	40ug	GENSCRIPT

전체보기

1
Maker	GENSCRIPT
Cat.No.	Z03502-100
Product	GenCRISPR™ Cas12a (Cpf1) Nuclease
Size	100ug
Qty
Data Sheet

2
Maker	GENSCRIPT
Cat.No.	Z03502-500
Product	GenCRISPR™ Cas12a (Cpf1) Nuclease
Size	500ug
Qty
Data Sheet

3
Maker	GENSCRIPT
Cat.No.	Z03502-1
Product	GenCRISPR™ Cas12a (Cpf1) Nuclease
Size	1mg
Qty
Data Sheet

4
Maker	GENSCRIPT
Cat.No.	Z03502-50
Product	GenCRISPR™ Cas12a (Cpf1) Nuclease
Size	50ug
Qty
Data Sheet

5
Maker	GENSCRIPT
Cat.No.	Z03486-100
Product	GenCRISPR™ Cas13a (C2c2) Nuclease
Size	100ug
Qty
Data Sheet

6
Maker	GENSCRIPT
Cat.No.	Z03486-1
Product	GenCRISPR™ Cas13a (C2c2) Nuclease
Size	1mg
Qty
Data Sheet

7
Maker	GENSCRIPT
Cat.No.	Z03486-500
Product	GenCRISPR™ Cas13a (C2c2) Nuclease
Size	500ug
Qty
Data Sheet

8
Maker	GENSCRIPT
Cat.No.	A01885-100
Product	GenCRISPR™ Cas9 Antibody, pAb, Rabbit
Size	100ul
Qty
Data Sheet

9
Maker	GENSCRIPT
Cat.No.	A01885-40
Product	GenCRISPR™ Cas9 Antibody, pAb, Rabbit
Size	40ul
Qty
Data Sheet

10
Maker	GENSCRIPT
Cat.No.	Z03386-50
Product	GenCRISPR™ Cas9 Nuclease
Size	50ug (5×10ug)(0.2mg/ml)
Qty
Data Sheet

11
Maker	GENSCRIPT
Cat.No.	Z03386-10
Product	GenCRISPR™ Cas9 Nuclease
Size	10ug (0.2mg/ml)
Qty
Data Sheet

12
Maker	GENSCRIPT
Cat.No.	Z03385-100
Product	GenCRISPR™ Cas9-C-NLS Nuclease
Size	100ug (4mg/ml)
Qty
Data Sheet

13
Maker	GENSCRIPT
Cat.No.	Z03385-50
Product	GenCRISPR™ Cas9-C-NLS Nuclease
Size	50ug (1mg/ml)
Qty
Data Sheet

14
Maker	GENSCRIPT
Cat.No.	Z03468-1
Product	GenCRISPR™ Cas9-N-NLS Nuclease
Size	1mg (10mg/ml)
Qty
Data Sheet

15
Maker	GENSCRIPT
Cat.No.	Z03388-50
Product	GenCRISPR™ Cas9-N-NLS Nuclease
Size	50ug (1mg/ml)
Qty
Data Sheet

16
Maker	GENSCRIPT
Cat.No.	Z03468-500
Product	GenCRISPR™ Cas9-N-NLS Nuclease
Size	500ug (10mg/ml)
Qty
Data Sheet

17
Maker	GENSCRIPT
Cat.No.	Z03388-100
Product	GenCRISPR™ Cas9-N-NLS Nuclease
Size	100ug (4mg/ml)
Qty
Data Sheet

18
Maker	GENSCRIPT
Cat.No.	Z03468-100
Product	GenCRISPR™ Cas9-N-NLS Nuclease
Size	100ug (10mg/ml)
Qty
Data Sheet

19
Maker	GENSCRIPT
Cat.No.	Z03470-300
Product	GenCRISPR™ eSpCas9-N-NLS Nuclease
Size	300ug (4mg/ml)
Qty
Data Sheet

20
Maker	GENSCRIPT
Cat.No.	Z03470-100
Product	GenCRISPR™ eSpCas9-N-NLS Nuclease
Size	100ug (4mg/ml)
Qty
Data Sheet

21
Maker	GENSCRIPT
Cat.No.	A01958-40
Product	GenCRISPR™ FnCpf1 Antibody (14H3), mAb, Mouse
Size	40ug
Qty
Data Sheet

22
Maker	GENSCRIPT
Cat.No.	A01957-40
Product	GenCRISPR™ FnCpf1 Antibody (9H6), mAb, Mouse
Size	40ug
Qty
Data Sheet

23
Maker	GENSCRIPT
Cat.No.	Z03390-50
Product	GenCRISPR™ NLS-Cas9-D10A Nickase
Size	50ug (1mg/ml)
Qty
Data Sheet

24
Maker	GENSCRIPT
Cat.No.	Z03390-100
Product	GenCRISPR™ NLS-Cas9-D10A Nickase
Size	100ug (4mg/ml)
Qty
Data Sheet

25
Maker	GENSCRIPT
Cat.No.	Z03390-10
Product	GenCRISPR™ NLS-Cas9-D10A Nickase
Size	10ug (1mg/ml)
Qty
Data Sheet

26
Maker	GENSCRIPT
Cat.No.	Z03467-1
Product	GenCRISPR™ NLS-Cas9-EGFP Nuclease
Size	1mg(10mg/ml)
Qty
Data Sheet

27
Maker	GENSCRIPT
Cat.No.	Z03393-50
Product	GenCRISPR™ NLS-Cas9-EGFP Nuclease
Size	50ug (1mg/ml)
Qty
Data Sheet

28
Maker	GENSCRIPT
Cat.No.	Z03467-500
Product	GenCRISPR™ NLS-Cas9-EGFP Nuclease
Size	500ug(10mg/ml)
Qty
Data Sheet

29
Maker	GENSCRIPT
Cat.No.	Z03393-100
Product	GenCRISPR™ NLS-Cas9-EGFP Nuclease
Size	100ug (3mg/ml)
Qty
Data Sheet

30
Maker	GENSCRIPT
Cat.No.	Z03467-100
Product	GenCRISPR™ NLS-Cas9-EGFP Nuclease
Size	100ug(10mg/ml)
Qty
Data Sheet

31
Maker	GENSCRIPT
Cat.No.	Z03389-50
Product	GenCRISPR™ NLS-Cas9-NLS Nuclease
Size	50ug (1mg/ml)
Qty
Data Sheet

32
Maker	GENSCRIPT
Cat.No.	Z03469-100
Product	GenCRISPR™ NLS-Cas9-NLS Nuclease
Size	100ug (10mg/ml)
Qty
Data Sheet

33
Maker	GENSCRIPT
Cat.No.	Z03389-100
Product	GenCRISPR™ NLS-Cas9-NLS Nuclease
Size	100ug (4mg/ml)
Qty
Data Sheet

34
Maker	GENSCRIPT
Cat.No.	Z03469-1
Product	GenCRISPR™ NLS-Cas9-NLS Nuclease
Size	1mg (10mg/ml)
Qty
Data Sheet

35
Maker	GENSCRIPT
Cat.No.	Z03469-500
Product	GenCRISPR™ NLS-Cas9-NLS Nuclease
Size	500ug (10mg/ml)
Qty
Data Sheet

36
Maker	GENSCRIPT
Cat.No.	Z03699-0.1
Product	GenCRISPR™ SaCas9 2NLS Nuclease
Size	0.1mg
Qty
Data Sheet

37
Maker	GENSCRIPT
Cat.No.	Z03699-1
Product	GenCRISPR™ SaCas9 2NLS Nuclease
Size	1mg
Qty
Data Sheet

38
Maker	GENSCRIPT
Cat.No.	Z03699-0.5
Product	GenCRISPR™ SaCas9 2NLS Nuclease
Size	0.5mg
Qty
Data Sheet

39
Maker	GENSCRIPT
Cat.No.	A01936-40
Product	GenCRISPR™ SpCas9 Antibody (14B6), mAb, Mouse
Size	40ug
Qty
Data Sheet

40
Maker	GENSCRIPT
Cat.No.	A01935-40
Product	GenCRISPR™ SpCas9 Antibody (4A1), mAb, Mouse
Size	40ug
Qty
Data Sheet

41
Maker	GENSCRIPT
Cat.No.	Z03396-250
Product	GenCRISPR™ T7 Endonuclease I
Size	250U (10000 U/ml)
Qty
Data Sheet

42
Maker	GENSCRIPT
Cat.No.	Z03396-1250
Product	GenCRISPR™ T7 Endonuclease I
Size	1250U (10000 U/ml)
Qty
Data Sheet

43
Maker	GENSCRIPT
Cat.No.	Z03624-GMP-5
Product	GenCRISPR™ Ultra eSpCas9-2NLS-GMP
Size	5mg
Qty
Data Sheet

44
Maker	GENSCRIPT
Cat.No.	Z03624-GMP-2.5
Product	GenCRISPR™ Ultra eSpCas9-2NLS-GMP
Size	2.5mg
Qty
Data Sheet

45
Maker	GENSCRIPT
Cat.No.	Z03623-GMP-2.5
Product	GenCRISPR™ Ultra NLS-Cas9-GMP
Size	2.5mg
Qty
Data Sheet

46
Maker	GENSCRIPT
Cat.No.	Z03623-GMP-5
Product	GenCRISPR™ Ultra NLS-Cas9-GMP
Size	5mg
Qty
Data Sheet

47
Maker	GENSCRIPT
Cat.No.	Z03621-0.5
Product	GenCRISPR™ Ultra NLS-Cas9-Research
Size	0.5mg
Qty
Data Sheet

전체보기

CRISPR Cas9 Nuclease | 고마바이오텍

CRISPR Cas9 Nuclease

주문정보

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