QuickFold™ Protein Refolding Buffer Formulation |
Component |
Formulation after Preparation (Addition of DTT,
GSH, and GSSH) |
DTT |
GSH/GSSH |
Buffer 1 |
50 mM MES pH
6.0, 9.6 mM NaCl, 0.4 mM KCl, 2 mM MgC , 2 mM CaC , 0.75 M Guanidine HCl,
0.5% Triton X-100, 0.1 mM DTT |
O |
|
Buffer 2 |
50 mM MES pH
6.0, 9.6 mM NaCl, 0.4 mM KCl, 2 mM MgC , 2 mM CaC , 0.5 M arginine, 0.05%
polyethylene glycol 3,550, 0.1 mM GSH, 0.01 mM GSSH |
|
O |
Buffer 3 |
50 mM MES pH
6.0, 9.6 mM NaCl, 0.4 mM KCl, 1 mM EDTA, 0.4 M sucrose, 0.75 M Guanidine HCl,
0.5% Triton X-100, 0.05% polyethylene glycol 3,550, 0.1 mM DTT |
O |
|
Buffer 4 |
50 mM MES pH
6.0, 240 mM NaCl, 10 mM KCl, 2 mM MgC , 2 mM CaC , 0.5 M arginine, 0.5%
Triton X-100, 0.1 mM GSH, 0.01 mM GSSH |
|
O |
Buffer 5 |
50 mM MES pH
6.0, 240 mM NaCl, 10 mM KCl, 1 mM EDTA, 0.4 M sucrose, 0.75 M Guanidine HCl,
0.1 mM DTT |
O |
|
Buffer 6 |
50 mM MES pH
6.0, 240 mM NaCl, 10 mM KCl, 1 mM EDTA, 0.5 M arginine, 0.4 M sucrose, 0.5%
Triton X-100, 0.05% polyethylene glycol 3,550, 0.1 mM GSH, 0.01 mM GSSH |
|
O |
Buffer 7 |
50 mM MES pH
6.0, 240 mM NaCl, 10 mM KCl, 2 mM MgC , 2 mM CaC , 0.75 M Guanidine HCl,
0.05% polyethylene glycol 3,550, 0.1 mM DTT |
O |
|
Buffer 8 |
50 mM Tris-Cl
pH 8.5, 9.6 mM NaCl, 0.4 mM KCl, 2 mM MgC , 2 mM CaC , 0.4 M sucrose, 0.5%
Triton X-100, 0.05% polyethylene glycol 3,550, 0.1 mM GSH, 0.01 mM GSSH |
|
O |
Buffer 9 |
50 mM Tris-Cl
pH 8.5, 9.6 mM NaCl, 0.4 mM KCl, 1 mM EDTA, 0.5 M arginine, 0.75 M Guanidine
HCl, 0.05% polyethylene glycol 3,550, 0.1 mM DTT |
O |
|
Buffer 10 |
50 mM Tris-Cl
pH 8.5, 9.6 mM NaCl, 0.4 mM KCl, 2 mM MgC , 2 mM CaC , 0.5 M arginine, 0.4 M
sucrose, 0.75 M Guanidine HCl, 0.1 mM GSH, 0.01 mM GSSH |
|
O |
Buffer 11 |
50 mM Tris-Cl
pH 8.5, 9.6 mM NaCl, 0.4 mM KCl, 1 mM EDTA, 0.5% Triton X-100, 0.1 mM DTT |
O |
|
Buffer 12 |
50 mM Tris-Cl
pH 8.5, 240 mM NaCl, 10 mM KCl, 1 mM EDTA, 0.05% polyethylene glycol 3,550,
0.1 mM GSH, 0.01 mM GSSH |
|
O |
Buffer 13 |
50 mM Tris-Cl
pH 8.5, 240 mM NaCl, 10 mM KCl, 1 mM EDTA, 0.5 M arginine, 0.75 M Guanidine
HCl, 0.5% Triton X-100, 0.1 mM DTT |
O |
|
Buffer 14 |
50 mM Tris-Cl
pH 8.5, 240 mM NaCl, 10 mM KCl, 2 mM MgC , 2 mM CaC , 0.5 M arginine, 0.4 M
sucrose, 0.75 M Guanidine HCl, 0.5% Triton X-100, 0.05% polyethylene glycol
3,550, 0.1 mM GSH, 0.01 mM GSSH |
|
O |
Buffer 15 |
50 mM Tris-Cl
pH 8.5, 240 mM NaCl, 10 mM KCl, 2 mM MgC , 2 mM CaC , 0.4 M sucrose, 0.1 mM
DTT |
O |
|