미세소관 형광체 | Microtubule Staining Dye | 고마바이오텍

특징 

• 미세소관은 모든 진핵 세포에서 발견되는 세포 골격의 주요 구성 요소입니다. 지름 20-25nm인 가운데가 비어있는 실린더 형태이며, α- 와 β-tubulin으로 구성되어 직선의 protofilament 형태를 이룹니다. GTP 가수분해를 통해 연속적으로 형성과 분해가 이어지며, 빠르게 성장하는 β-subunits (+) end와 느리게 성장하는 α-subunits (-) end를 가집니다.  
• 미세소관은 다양한 세포 과정에서 중요한 역할을 합니다. 세포 내 수송, 신호 전달, 증식 등의 세포 필수적 작용에 관여하며 세포 구조를 유지합니다. 또 세포 분열 동안 진핵 세포의 염색체를 끌어당기는 mitotic spindle을 구성합니다.
• 미세소관 연구에 유용한 Taxol 기반의 Flutax와 Taxol Janelia Fluor를 제공합니다. 

주요 제품  

참고 데이터

Flutax 1 labeled HeLa cells. 

Flutax 1 was diluted in HBSS and applied to live HeLa cells at a concentration of 2 μM. HeLa cells were incubated for 1 hour at 37°C. After incubation was complete, Flutax 1 was washed off, and fresh HBSS was applied to the cells. Cells were not fixed because Flutax 1 staining is not retained at all after fixation (photobleach). Staining can be done only on Live cells. Flutax 1 staining in live cells diminishes very rapidly when exposed to light, so the cells should be exposed to light as little as possible to retain good fluorescent signal. 

Flutax 2 labeled HeLa cells. 

Flutax 2 was diluted in HBSS and applied to live HeLa cells at a concentration of 2 μM. HeLa cells were incubated for 1 hour at 37°C. After incubation was complete, Flutax 2 was washed off, and fresh HBSS was applied to the cells. Cells were not fixed because Flutax 2 staining is not retained at all after fixation (photobleach). Like Flutax 1, Flutax 2 staining in live cells diminishes very rapidly when exposed to light, so the cells should be exposed to light as little as possible to retain good signal. 

Application of Taxol Janelia Fluor® 646, dye on COS7 cells. 

COS7 cells were labeled with 3 µM Taxol Janelia Fluor® 646 for one hour at 37°C. Images were taken on a Leica TCS SP8 Confocal Laser Scanning Microscope. All images kindly provided by Prof. Christian Soeller, University of Exeter; acquired by Evelina Lucinskaite, Anna Meletiou and Alexander Clowsley.