TrueBlack Lipofuscin Autofluorescence Quenchers | 고마바이오텍

Figure 1. Lipofuscin autofluorescence in methanol-fixed adult human brain sections. In untreated tissue (top row), lipofuscin appeared as fluorescent granules that fluoresced in all fluorescence channels. Sudan Black B (middle row) masked lipofuscin autofluorescence, but introduced background in the red and far-red channels. TrueBlack® (bottom row) masked lipofuscin with minimal increase in fluorescence background (bottom row). Methanol-fixed cryosections of adult human cerebral cortex were left untreated or stained with 0.1% Sudan Black B in 70% ethanol or 1X TrueBlack® according to product protocol. Samples were imaged at the same gain settings on a Zeiss LSM 700 confocal microscope in the FITC (green), Cy®3 (red), and Cy®5 (far-red) channels.

Figure 2. Quenching of lipofuscin autofluorescence using TrueBlack® pretreatment before immunofluorescence staining. Formaldehyde-fixed human cortex cryosections were left untreated (A) or treated with TrueBlack® (B), then stained with CF®488A anti-NeuN antibody conjugate (green) and DAPI (blue). Sections were imaged in all channels on a Zeiss LSM700 confocal microscope. A. Lipofuscin fluoresces brightly in all channels, appearing as white spots (red arrows)  in the merged image of untreated tissue. B. TrueBlack® pretreatment eliminated lipofuscin autofluorescence, with negligible effect on specific staining.