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Immunoglobulin Isotype Control 이미지

Immunoglobulin Isotype Control

ELISA, WB등에 이용되는 Immunoglobulin isotype control입니다.

배경

Immunoassay를 통해 타겟을 감지하려고 할 때, 타겟과 항체의 특이적 반응 외에도 Fc 수용체, 내인성 효소, 반응성 항원 결정기 등에 항체가 결합하기도 합니다. 이러한 비특이적 결합에 의해 백그라운드 staining이 되면 실험 결과에 왜곡이 생기게 됩니다. 백그라운드 염색이나 위양성 결과를 줄이기 위해, 음성 대조군이 필요합니다. 항체와 유사하지만, 타겟과 결합하지 않는 immunoglobulin control을 사용하면, ELISA, IHC, WB에서 실험 결과의 정확도를 향상시킬 수 있습니다.



특징

  • Flow cytometry, IHC, ELISA, WB, IF 등에 이용되는 Immunoglobulin isotype control입니다. 
  • 1차 항체와 유사하지만, 타겟 특이적 결합을 하지 않아 negative control로 사용할 수 있습니다.
  • Normal Human IgG 는  멀티플렉스와 microarray에서 대조군으로 ELISA에서 standard로 사용할 수 있습니다.
  • Normal Human IgM Lambda는 ELISA에서 standard로 사용할 수 있습니다.
  • Isotype control은 blocking이나 coating agent로도 사용할 수 있습니다. 

 



주요 제품



Control Normal Human IgG

 

  • CATALOG NUMBER: 98-217
  • Tested Applications: ELISA, IP, Microarray, Multiplex
  • Host Species: Human
  • Size: 1 mg
  • HOST SPECIES: Human
  • TESTED APPLICATIONS: ELISA, IP, Microarray, Multiplex
  • APPLICATION NOTE: Normal Human IgG can be used as control in multiplex and microarray assays and as a standard in ELISA.
 


Human IgG-UNLB (98-217) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Mouse Anti-Human IgG Fc-UNLB followed by Goat Anti-Mouse IgG, Human ads-HRP secondary antibody and chemiluminescent detection. 



Control Normal Human IgG1 Kappa

 

  • CATALOG NUMBER: 98-221
  • Tested Applications: ELISA, Flow, In vitro Control, IP, Surface Plasma Resonance
  • Host Species: Human
  • Size: 0.5 mg
  • HOST SPECIES: Human
  • TESTED APPLICATIONS: ELISA, Flow, In vitro Control, IP, Surface Plasma Resonance
  • APPLICATION NOTE: Normal Human IgG1 Kappa can be used as control in flow cytometry, immunoprecipitation, and in vitro assays, as a standard in ELISA, and for immobilization in surface plasmon resonance.

 


ELISA plate was coated with Human IgG1 Kappa (98-221). Immunoglobulin was detected with serially diluted Mouse Anti-Human IgG1 Hinge-BIOT, Mouse Anti-Human IgG2 Fc-BIOT, Mouse Anti-Human IgG3 Hinge-BIOT, Mouse Anti-Human IgG4 pFc-BIOT, Mouse Anti-Human IgA1-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA2-BIOT, Mouse Anti-Human IgD-BIOT, Mouse Anti-Human IgE Fc-BIOT, Mouse Anti-Human IgM-BIOT, Mouse Anti-Human Kappa-BIOT, and Mouse Anti-Human Lambda-BIOT followed by Streptavidin-HRP.  



Control Normal Goat IgG (FITC)

 

  • CATALOG NUMBER: 98-105
  • Tested Applications: FLISA, Flow, IHC-Fr, IHC-P
  • Host Species: Goat
  • Size: 100 tests 
  • HOST SPECIES: Goat
  • TESTED APPLICATIONS: FLISA, Flow, IHC-Fr, IHC-P
  • APPLICATION NOTE: Normal Goat IgG (FITC) can be used as control in flow cytometry and immunohistochemistry assays.  




Frozen mouse lymph node section was stained with Goat IgG-FITC (98-105: left) and Goat Anti-Mouse IgM, Human ads-FITC (right) followed by DAPI. 


 


주문정보

주문정보 - Cat No, PRODUCT, SIZE, 수량 등 항목으로 구성되어있습니다.
  Product Cat.No. Size Maker Qty Data Sheet MSDS
Chicken IgY Isotype Control antibody ARG20781 1 mg ARIGO
Chicken IgY Isotype Control antibody (HRP) ARG20782 500 ul ARIGO
Control Normal Canine IgG 98-209 5 mg PROSCI
Control Normal Chicken IgY 98-240 10 mg PROSCI
Control Normal Chicken IgY 3707 1 mg PROSCI
Control Normal Chicken IgY (HRP) 98-241 1 ml PROSCI
Control Normal Donkey IgG 98-242 10 mg PROSCI
Control Normal Donkey IgG 5087 1 mg PROSCI
Control Normal Goat F(ab’)2 IgG 98-114 5 mg PROSCI
Control Normal Goat F(ab’)2 IgG (APC) 98-118 100 tests PROSCI
Control Normal Goat F(ab’)2 IgG (Biotin) 98-116 0.5 mg PROSCI
Control Normal Goat F(ab’)2 IgG (Cy5) 98-121 100 tests PROSCI
Control Normal Goat F(ab’)2 IgG (FITC) 98-115 100 tests PROSCI
Control Normal Goat F(ab’)2 IgG (Low Endotoxin/Azide Free) 98-120 0.5 mg PROSCI
Control Normal Goat F(ab’)2 IgG (PE) 98-117 100 tests PROSCI
Control Normal Goat F(ab’)2 IgG (PE-Cy5) 98-119 100 tests PROSCI
Control Normal Goat IgG 3705 1 mg PROSCI
Control Normal Goat IgG 98-104 10 mg PROSCI
Control Normal Goat IgG (APC) 98-110 100 tests PROSCI
Control Normal Goat IgG (Biotin) 98-108 0.5 mg PROSCI
Control Normal Goat IgG (FITC) 98-105 100 tests PROSCI
Control Normal Goat IgG (Low Endotoxin/Azide Free) 98-112 0.5 mg PROSCI
Control Normal Goat IgG (PE) 98-109 100 tests PROSCI
Control Normal Goat IgG (PE-Cy5) 98-111 100 tests PROSCI
Control Normal Goat IgG (Rhodamine) 98-106 100 tests PROSCI
Control Normal Goat IgG (Texas Red) 98-107 100 tests PROSCI
Control Normal Goat IgG Fab 98-211 5 mg PROSCI
Control Normal Goat IgG Fc 98-210 2 mg PROSCI
Control Normal Hamster IgG 98-195 0.5 mg PROSCI
Control Normal Hamster IgG (APC) 98-199 0.1 mg PROSCI
Control Normal Hamster IgG (APC-Cy5.5) 98-205 0.1 mg PROSCI
Control Normal Hamster IgG (Biotin) 98-197 0.5 mg PROSCI
Control Normal Hamster IgG (Cy5) 98-202 0.1 mg PROSCI
Control Normal Hamster IgG (FITC) 98-196 0.5 mg PROSCI
Control Normal Hamster IgG (Low Endotoxin/Azide Free) 98-201 0.5 mg PROSCI
Control Normal Hamster IgG (PE) 98-198 0.1 mg PROSCI
Control Normal Hamster IgG (PE-Cy5) 98-200 0.1 mg PROSCI
Control Normal Hamster IgG (PE-Cy7) 98-204 0.1 mg PROSCI
Control Normal Horse IgG 5089 1 mg PROSCI
Control Normal Human IgA Kappa 98-231 0.5 mg PROSCI
Control Normal Human IgA Kappa (Alkaline Phosphatase) 98-232 1 ml PROSCI
Control Normal Human IgA Kappa (HRP) 98-233 1 ml PROSCI
Control Normal Human IgA Lambda 98-234 0.5 mg PROSCI
Control Normal Human IgA Lambda (Alkaline Phosphatase) 98-235 1 ml PROSCI
Control Normal Human IgA Lambda (HRP) 98-236 1 ml PROSCI
Control Normal Human IgG 98-217 1 mg PROSCI
Control Normal Human IgG 5503 1 mg PROSCI
Control Normal Human IgG (Alkaline Phosphatase) 98-218 1 ml PROSCI
Control Normal Human IgG (HRP) 98-219 1 ml PROSCI
Control Normal Human IgG (Low Endotoxin/Azide Free) 98-220 0.5 mg PROSCI
전체보기
Maker
ARIGO
Cat.No.
ARG20781
Product
Chicken IgY Isotype Control antibody
Size
1 mg
Qty
Data Sheet
Maker
ARIGO
Cat.No.
ARG20782
Product
Chicken IgY Isotype Control antibody (HRP)
Size
500 ul
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-209
Product
Control Normal Canine IgG
Size
5 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-240
Product
Control Normal Chicken IgY
Size
10 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
3707
Product
Control Normal Chicken IgY
Size
1 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-241
Product
Control Normal Chicken IgY (HRP)
Size
1 ml
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-242
Product
Control Normal Donkey IgG
Size
10 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
5087
Product
Control Normal Donkey IgG
Size
1 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-114
Product
Control Normal Goat F(ab’)2 IgG
Size
5 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-118
Product
Control Normal Goat F(ab’)2 IgG (APC)
Size
100 tests
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-116
Product
Control Normal Goat F(ab’)2 IgG (Biotin)
Size
0.5 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-121
Product
Control Normal Goat F(ab’)2 IgG (Cy5)
Size
100 tests
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-115
Product
Control Normal Goat F(ab’)2 IgG (FITC)
Size
100 tests
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-120
Product
Control Normal Goat F(ab’)2 IgG (Low Endotoxin/Azide Free)
Size
0.5 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-117
Product
Control Normal Goat F(ab’)2 IgG (PE)
Size
100 tests
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-119
Product
Control Normal Goat F(ab’)2 IgG (PE-Cy5)
Size
100 tests
Qty
Data Sheet
Maker
PROSCI
Cat.No.
3705
Product
Control Normal Goat IgG
Size
1 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-104
Product
Control Normal Goat IgG
Size
10 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-110
Product
Control Normal Goat IgG (APC)
Size
100 tests
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-108
Product
Control Normal Goat IgG (Biotin)
Size
0.5 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-105
Product
Control Normal Goat IgG (FITC)
Size
100 tests
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-112
Product
Control Normal Goat IgG (Low Endotoxin/Azide Free)
Size
0.5 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-109
Product
Control Normal Goat IgG (PE)
Size
100 tests
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-111
Product
Control Normal Goat IgG (PE-Cy5)
Size
100 tests
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-106
Product
Control Normal Goat IgG (Rhodamine)
Size
100 tests
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-107
Product
Control Normal Goat IgG (Texas Red)
Size
100 tests
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-211
Product
Control Normal Goat IgG Fab
Size
5 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-210
Product
Control Normal Goat IgG Fc
Size
2 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-195
Product
Control Normal Hamster IgG
Size
0.5 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-199
Product
Control Normal Hamster IgG (APC)
Size
0.1 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-205
Product
Control Normal Hamster IgG (APC-Cy5.5)
Size
0.1 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-197
Product
Control Normal Hamster IgG (Biotin)
Size
0.5 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-202
Product
Control Normal Hamster IgG (Cy5)
Size
0.1 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-196
Product
Control Normal Hamster IgG (FITC)
Size
0.5 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-201
Product
Control Normal Hamster IgG (Low Endotoxin/Azide Free)
Size
0.5 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-198
Product
Control Normal Hamster IgG (PE)
Size
0.1 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-200
Product
Control Normal Hamster IgG (PE-Cy5)
Size
0.1 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-204
Product
Control Normal Hamster IgG (PE-Cy7)
Size
0.1 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
5089
Product
Control Normal Horse IgG
Size
1 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-231
Product
Control Normal Human IgA Kappa
Size
0.5 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-232
Product
Control Normal Human IgA Kappa (Alkaline Phosphatase)
Size
1 ml
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-233
Product
Control Normal Human IgA Kappa (HRP)
Size
1 ml
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-234
Product
Control Normal Human IgA Lambda
Size
0.5 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-235
Product
Control Normal Human IgA Lambda (Alkaline Phosphatase)
Size
1 ml
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-236
Product
Control Normal Human IgA Lambda (HRP)
Size
1 ml
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-217
Product
Control Normal Human IgG
Size
1 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
5503
Product
Control Normal Human IgG
Size
1 mg
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-218
Product
Control Normal Human IgG (Alkaline Phosphatase)
Size
1 ml
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-219
Product
Control Normal Human IgG (HRP)
Size
1 ml
Qty
Data Sheet
Maker
PROSCI
Cat.No.
98-220
Product
Control Normal Human IgG (Low Endotoxin/Azide Free)
Size
0.5 mg
Qty
Data Sheet
전체보기

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