Topoisomerase Related Drugs | 고마바이오텍

10-Hydroxy Camptothecin has shown promising activity against a range of tumour types. This CPT derivative causes cell toxicity by stabilizing a ternary complex between the nuclear enzyme topoisomerase I (topo I) and double-stranded DNA, leading to single and double strand breaks. TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase I. 

9-Amino-20-(R,S)-camptothecin (also known as 9-amino campthecin) is an analogue of camptothecin.  Like other CPT congeners, it shows some promising activity against a range of tumor types.  This compound causes cell toxicity by stabilizing a ternary complex between the nuclear enzyme topoisomerase I (topo I) and double-stranded DNA, leading to single and double strand breaks.  TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase I. 

20(S)camptothecin has shown promising activity against a range of tumour types.  This CPT derivative causes cell toxicity by stabilizing a ternary complex between the nuclear enzyme topoisomerase I (topo I) and double-stranded DNA, leading to single and double strand breaks.  TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase I. 

Camptothecin is a topo I poison that both inhibits enzyme activity and stabilizes formation of topo I cleavage complexes (Hsiang et al J. Biol. Chem. 260:14873-14878, 1985).  TopoGEN provides this reagent as a control inhibitor/poison of topoisomerase I.   

Hycamtin (topotecan hydrochloride) is a semi-synthetic derivative of camptothecin and is an anti-tumor drug that targets topo I and is a topo I poison (induces cleavage complexes). 

TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II.  For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of assay being employed (SDSK+ vs. Radioactive End Labeled DNA vs. testing for plasmid cleavages).  Note that large amounts of enzyme will be required to detect cleavage complex, compared to assays that measure catalytic activity.     

TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II.     

TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II.  For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of assay being employed (SDSK+ vs. Radioactive End Labeled DNA vs. testing for plasmid cleavages).  Note that large amounts of enzyme will be required to detect cleavage complex, compared to assays that measure catalytic activity.   

Ellipticine is a topoisomerase II poison that induces topo II dependent cleavages. 

TopoGEN provides this reagent as a control poison/inhibitor of topoisomerase II.  For in vitro cleavage detection, we recommend that the drug be tested over a wide range of uM concentrations; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of assay being employed (SDSK+ vs. Radioactive End Labeled DNA vs. testing for plasmid cleavages).  Note that large amounts of enzyme will be required to detect cleavage complex, compared to assays that measure catalytic activity. 

Genistein is an isoflavone and an inhibitor of tyrosine protein kinases.  It has been reported to induce topo II dependent cleavages.   

HU-331 is a cannabinoid-based quinone with potential anticancer activity.     

A control poison/inhibitor of DNA Gyrase and Topo IV.  This is also included in the Topo IV/Gyrase Drug Screening Kits.