피지 세포 | Human iPSC-Derived Sebocytes | 고마바이오텍

특징  

• 피부과 연구, 약리학, 독성학 및 신약개발 연구에 이용될 수 있는 Human iPSC 유래 피지 세포 입니다.
• 5세 남성 기증자의 PBMC에서 생성된 iPSC로 제작되었습니다.
• 피지세포의 특이 마커인 MUC1, KRT7과, 지질 활성화 전사인자인 PPARG-γ, FADS2, androgen receptors (AR), melanocortin 5 receptor (MC5R)를 발현합니다.
• Testosterone (테스토스테론)과 linoleic acid (리놀레산)에 기능적으로 반응합니다. 
• Number of cells : ≥2 million
• Donor : Male, 5 years old
• Genetics : Healthy control

axoCells Sebocytes express the key markers MUC1 (MUCIN 1) and KRT7 (KERATIN 7).

PCI-SEB sebocytes exhibit >90% purity as measured by KRT7+ expression. Black: isotype control ; Red: anti-KRT7 antibody.

Evolution of specific markers after 3 (d3) and 5 (d5) days in culture compared to primary keratinocytes (Ker). Functional markers such as lipid activated transcription factor PPARG-γ, FADS2 (fatty acid biosynthesis pathway), androgen receptors (AR) and melanocortin 5 receptor (MC5R) are strongly expressed after 5 day.

PCi-SEB respond to 24h treatment with linoleic acid (LA) by a dose-dependent (up to 5-fold) lipid accumulation (Bodipy staining).

PCI-SEB sebocytes respond to a 96h treatment with testosterone (10 µM) by a 2-fold increase in lipid content. This response is significantly inhibited by the 5-α-reductase inhibitor finasteride (10 µM).