고마바이오텍(주)

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제품 상세

Antibody

6.5 - 270 kDa 범위를 커버하는 3가지 컬러 밴드를 포함하며,
최대 100%까지의 transfer 효과를 개런티하는 Prestained protein ladder를 소개합니다.

프린트 제품 문의

Phospholipid란? 

 

  • Phospholipid는 cell membrane과 lipid bilayer의 주요 구성 성분으로, hydrophilic head와 hydrophobic tail로 구성되어 있고, choline group, phosphate group, 그리고 diaglyceride나 sphingosine을 함유합니다. 


phospholipid란?


주요 제품

 


Phoaphatidylcholine Assay Kit (#STA-600) 

 

  • serum, plasma, tissue 샘플에 존재하는 phosphatidylcholine을 측정합니다.
  • phosphatidylcholine-specific phospholipase D enzyme과 choline oxidase를 이용하여 분해된 산물을 형광법으로 확인합니다.
  • 민감도 : > 0.5 mg/dL phosphatidylcholine 측정

Phoaphatidylcholine Assay Kit (#STA-600)



 

Sphingomyelin Assay Kit (#STA-601)

  • serum, plasma, tissue 샘플에 존재하는 sphingomyelin을 측정합니다.
  • sphingomyelinase, alkaline phosphatase, choline oxidase를 이용하여 분해된 산물을 형광법으로 확인합니다.

 

Sphingomyelin Assay Kit (#STA-601)




Diacylglycrol (DAG) Assay Kit (#MET-5028)

  • Coupled enzymatic reaction system을 통해 cell lysate 내에 있는 DAG의 농도를 측정합니다.
  • 민감도 : > 4uM DAG 측정

Diacylglycrol (DAG) Assay Kit (#MET-5028)





Total Phosphatidic Assay (#MET-5019)

  • Coupled enzymatic reaction system을 통해 cell lysate 내에 있는 toal phoaphatidic acid (PA and LPA)의 농도를 측정하는 kit 입니다.
  • 민감도 : > 5 uM PA 측정

Total Phosphatidic Assay (#MET-5019)




Phospholipid Assay Kit (#MET-5085)

  • 혈청, 혈장, 조직샘플 또는 세포현탁액에서 choline-based phospholipids 의 양을 측정합니다.
  • 샘플과 standard 는 37ºC에서 30분동안 반응 후, 96-well fluorometric plate reader 로 읽습니다.
  • detection sensitivity limit : 약 150 nM




사용 논문

STA-600 　
    1. Kumar, S. et al. (2021). Cocaine-Specific Effects on Exosome Biogenesis in Microglial Cells. Neurochem Res. doi: 10.1007/s11064-021-03231-2.
    2. Kim, Y. et al. (2020). Tumor-Targeting Liposomes with Transient Holes Allowing Intact Rituximab Internally. Biomacromolecules. doi: 10.1021/acs.biomac.0c01514.
    3. Jubinville, É. et al. (2020). Critical importance of dietary methionine and choline in the maintenance of lung homeostasis during normal and cigarette smoke exposure conditions. Am J Physiol Lung Cell Mol Physiol. doi: 10.1152/ajplung.00353.2019.
    4. Kim, J. et al. (2020). Topological Adaptation of Transmembrane Domains to the Force-Modulated Lipid Bilayer Is a Basis of Sensing Mechanical Force. Curr Biol. pii: S0960-9822(20)30201-3. doi: 10.1016/j.cub.2020.02.028.
    5. Sørensen, D.M. et al. (2019). The P5A ATPase Spf1p is stimulated by phosphatidylinositol 4-phosphate and influences cellular sterol homeostasis. Mol Biol Cell. 30(9):1069-1084. doi: 10.1091/mbc.E18-06-0365.
    6. Tanaka, N. et al. (2017). Growth arrest and DNA damage-inducible 45α protects against nonalcoholic steatohepatitis induced by methionine- and choline-deficient diet. Biochim Biophys Acta. pii: S0925-4439(17)30299-5. doi: 10.1016/j.bbadis.2017.08.017.
    7. Park, E. S. et al. (2014). Phosphatidylcholine alteration identified using MALDI imaging MS in HBV-infected mouse livers and virus-mediated regeneration defects. PLoS One.  9:e103955.
STA-601 　
    1. Kumar, S. et al. (2021). Cocaine-Specific Effects on Exosome Biogenesis in Microglial Cells. Neurochem Res. doi: 10.1007/s11064-021-03231-2.
    2. Ledet, M.M. et al. (2020). Secreted sphingomyelins modulate low mammary cancer incidence observed in certain mammals. Sci Rep. 10(1):20580. doi: 10.1038/s41598-020-77639-1.
    3. Jacob, S. et al (2017). Assessment of plasma biomarkers for their association with Multiple Sclerosis progression. Journal of Neuroimmunology. doi: 10.1016/j.jneuroim.2017.01.008.
    4. Winkler, E.A. et al. (2014). Blood-spinal cord barrier disruption contributes to early motor-neuron degeneration in ALS-model mice. PNAS 111:E1035-E1042.
MET-5028 　
    1. Chiang, Y.P. et al. (2021). Sphingomyelin synthase related protein is a mammalian phosphatidylethanolamine phospholipase C. Biochim Biophys Acta Mol Cell Biol Lipids. 1866(11):159017. doi: 10.1016/j.bbalip.2021.159017.
    2. Brekk, O.R. et al. (2020). Cell type-specific lipid storage changes in Parkinson's disease patient brains are recapitulated by experimental glycolipid disturbance. Proc Natl Acad Sci U S A. doi: 10.1073/pnas.2003021117.
    3. Koundouros, N. et al. (2020). Metabolic Fingerprinting Links Oncogenic PIK3CA with Enhanced Arachidonic Acid-Derived Eicosanoids. Cell. doi: 10.1016/j.cell.2020.05.053.
    4. Day, P. et al. (2019). Inhibitors of diacylglycerol metabolism suppress CCR2 receptor signalling in human monocytes. Br J Pharmacol. doi: 10.1111/bph.14695.
    5. Badi, R.M. et al. (2019). Resveratrol protects against hepatic insulin resistance in a rat's model of non-alcoholic fatty liver disease by down-regulation of GPAT-1 and DGAT2 expression and inhibition of PKC membranous translocation. Clin Exp Pharmacol Physiol. doi: 10.1111/1440-1681.13074.
    6. Garcia, D. et al. (2019). Genetic Liver-Specific AMPK Activation Protects against Diet-Induced Obesity and NAFLD. Cell Rep. 26(1):192-208.e6. doi: 10.1016/j.celrep.2018.12.036.
    7. Fanning, S. et al. (2019). Lipidomic Analysis of α-Synuclein Neurotoxicity Identifies Stearoyl CoA Desaturase as a Target for Parkinson Treatment. Mol Cell. 73(5):1001-1014.e8. doi: 10.1016/j.molcel.2018.11.028.
    8. Kang, R. et al. (2018). Lipid Peroxidation Drives Gasdermin D-Mediated Pyroptosis in Lethal Polymicrobial Sepsis. Cell Host Microbe. 24(1):97-108.e4. doi: 10.1016/j.chom.2018.05.009.
    9. Tanaka, N., et al. (2017). Growth arrest and DNA damage-inducible 45α protects against nonalcoholic steatohepatitis induced by methionine- and choline-deficient diet. Biochim Biophys Acta. pii: S0925-4439(17)30299-5. doi: 10.1016/j.bbadis.2017.08.017.
MET-5019 　
    1. Li, R.H. et al. (2021). A phosphatidic acid-binding lncRNA SNHG9 facilitates LATS1 liquid-liquid phase separation to promote oncogenic YAP signaling. Cell Res. doi: 10.1038/s41422-021-00530-9.
    2. Nadeem, A. et al. (2021). Phosphatidic acid-mediated binding and mammalian cell internalization of the Vibrio cholerae cytotoxin MakA. PLoS Pathog. 17(3):e1009414. doi: 10.1371/journal.ppat.1009414.
    3. Zhang, G. et al. (2020). CD4+ T Cell-Mimicking Nanoparticles Broadly Neutralize HIV-1 and Suppress Viral Replication through Autophagy. mBio. 11(5):e00903-20. doi: 10.1128/mBio.00903-20.
    4. Loh, J. et al. (2019). An acute decrease in plasma membrane tension induces macropinocytosis via PLD2 activation. J Cell Sci. 132(17). pii: jcs232579. doi: 10.1242/jcs.232579.
    5. Kumar Sah, R. et al. (2019). Phosphatidic acid homeostasis regulated by a type-2 phosphatidic acid phosphatase represents a novel druggable target in malaria intervention. Cell Death Discov. 5:107. doi: 10.1038/s41420-019-0187-1.
    6. Zhang, P. et al. (2019). Lipin 2/3 phosphatidic acid phosphatases maintain phospholipid homeostasis to regulate chylomicron synthesis. J Clin Invest. 129(1):281-295. doi: 10.1172/JCI122595.
    7. Han, H. et al. (2018). Regulation of the Hippo Pathway by Phosphatidic Acid-Mediated Lipid-Protein Interaction. Mol Cell. 72(2):328-340.e8. doi: 10.1016/j.molcel.2018.08.038.
    8. Lutkewitte, A.J. et al. (2018). Lipin deactivation after acetaminophen overdose causes phosphatidic acid accumulation in liver and plasma in mice and humans and enhances liver regeneration. Food Chem Toxicol. 115:273-283. doi: 10.1016/j.fct.2018.03.014.
MET-5085 　
    1. Kumar, S. et al. (2021). Cocaine-Specific Effects on Exosome Biogenesis in Microglial Cells. Neurochem Res. doi: 10.1007/s11064-021-03231-2.
    2. Deng, Q. et al. (2020). Dietary Lactic Acid Bacteria Modulate Yolk Components and Cholesterol Metabolism by Hmgr Pathway in Laying Hens. Braz. J. Poult. 22(3):eRBCA-2020-1261. doi: 10.1590/1806-9061-2020-1261.
    3. Ahmadpour, A. et al. (2019). Study of the Circulatory Energy Balance Indicators and Hepatic Fat Content in Dromedary Camel during Late Pregnancy and Early Lactation. Small Ruminant Research. doi: 10.1016/j.smallrumres.2019.08.018.

주문정보

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제품 문의

 0.5 ml Elite Pre-stained Protein Ladder (2 x 0.25 ml) PAL-EPL-500 0.5ml 500
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