폐수, 생활하수의 오염검사를 위한 qPCR Kit | 고마바이오텍

CrAssPhage qPCR Assay Kit (#PNP-CRPH)  

• CrAssphage는 인간의 배설물과 폐수에 매우 풍부한 이중 가닥 DNA 바이러스입니다.
• qPCR 반응을 통해 다양한 DNA 병원체(예: 박테리아, 곰팡이 및 DNA 바이러스)의 DNA를 검출할 때 신뢰할 수 있는 내부 control로 사용됩니다.
• CrAssphage 게놈 DNA를 타겟으로 하는 primer/probe mixture를 제공합니다. 
• 형광 Probe는 HEX를, quencher는 BHQ-1를 이용합니다. 
• TaqMan 3 cleavage 메카니즘을 이용하므로, 5'-exonuclease activity를 갖는 DNA polymerase와 함께 사용합니다. 
• 구성품 : 
• Tube 1: 20X Primer/Probe mix specific for CrAssphage genomic DNA.
• Tube 2: 5000 copies/µl Positive control of synthetic 500 bp DNA fragment of CrAssphage (NC_02471).
• Tube 3 (옵션) : qPCR MasterMix 

Verification experiments (in a 2-plex reaction of CrAssphage and MPX1 products) with various added samples (given in text boxes for each panel).  

(1) No template control (NTC) with water – no PCR  reaction observed. 

(2) CrAssphage DNA construct where only HEX signal was observed (no FAM signal). 

(3) Monkeypox DNA construct where only FAM signal was observed (no HEX signal). 

(4) Bottom panel: both DNA constructs for CrAssphage and Monkeypox were added and both FAM and HEX signals were observed.

Verification experiments with water (no reaction observed) and three RNA extract samples from wastewater from three different locations in Michigan (for PCR each sample was 10X diluted). The HEX probe detects PMMoV genomic RNA.