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Immobilon Transfer Membranes

Immobilon Transfer Membranes are robust, versatile PVDF substrates for a variety of applications, including westerns, dot/slot blots, protein sequencing,and MS analysis.

Superior Handling

Unlike traditional blotting membranes, Immobilon membranes won’t crack or curl, and they can be cut without fracturing. They are also compatible with a variety of detection chemistries, including radioactive, chromogenic, chemiluminescent, fluorescent, and chemifluorescent techniques.

Excellent Protein Binding and High Signal

Immobilon membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, membranes optimized for fluorescent blots dramatically increase signal for high sensitivity in quantitative, multiplexing applications.

Optimized to Applications

Three Immobilon membrane types are optimized for your applications and preferred detection chemistries.

Immobilon-P Membrane

The original, and most commonly used, PVDF transfer membrane for western blotting. It has a 0.45 μm pore size and is recommended for blotting proteins 20 kDa. It’s available in sheets, rolls, and pre-cut blotting sandwiches.

Immobilon-PSQ Membrane

With its 0.2 μm pore size, this membrane has higher protein adsorption and sequencing yields than other membranes. Recommended for blotting proteins < 20 kDa and sequencing.

Immobilon-FL Membrane

The first transfer membrane optimized for fluorescence applications, with extremely low background fluorescence. Ideal for multiplexing. It has a 0.45 μm pore size and is compatible with all commonly used fluorescent dyes as well as non-fluorescent detection technologies

Immobilon-P Membrane is Compatible with a Variety of Stains

Immobilon-P Membrane is Compatible with a Variety of Stains

Calf liver proteins were visualized after electroblotting to Immobilon-P membranes: (A) Transillumination, (B) Coomassie™ Brilliant Blue, (C) Ponceau-S red, (D) Amido black and (E) CPTS total protein stains. Left to right, molecular weight standards and 12.2 μg, 6.1 μg, 3.1 μg of the lysate per lane.

출처: http://www.millipore.com