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 제품안내  세포배양   Stem Cell

hiPSC-Derived Macrophages

Axol iPSC 유래 대식세포는 냉동이 아닌 live 상태로 제공되어 염증 반응 및 면역 반응 모델링을 위한 분석 툴로 사용됩니다.

제품 문의하기

  • iPSC유도 대식세포를 제공합니다.
  • 냉동이 아닌 live 상태로 96well plate에 제공되어 분석에 바로 이용할 수 있습니다.
  • phagocytosis assays, flow-cytometry, ICC 등의 실험을 통해 cell surface marker인 CD14, CD11b, CD11c와 macrophage-specific calcium-binding protein인 IBA1의 발현을 확인하였습니다.
  • 염증 반응 및 면역 반응 모델링을 위한 분석 툴로 사용됩니다.

 Human iPSC-derived macrophages 
 Cat. No. Product Name Quantity
 ax0601 Human iPSC-Derived Macrophages (Male) 96-well plate
 ax0606 Human iPSC-Derived Macrophages (Female) 96-well plate

 Macrophage culture media and reagents
 Cat. No. Product Name Quantity
 ax0600 Macrophage Maintenance Medium 250 ml

  • Phase Contrast

   Phase contrast images of Axol Human iPSC-Derived Macrophages at days 3 (10x) and 7 (10x and 40x)
    post-differentiation. Human iPSC-derived monocytes are seeded using Macrophage Maintenance Medium (ax0600)
    into 96-well plates at a density of 50,000 cells/cm2. During maturation the spherical monocytes adhere strongly to
    the culture surface and as they differentiate to macrophages their morphology becomes rounded and elongated.
    Axol iPSC-Derived Macrophages show numerous large vesicles indicative of phagocytic entities.

  • Immunohistochemistry

  Expression of the IBA1 protein was confirmed in Axol iPSC-Derived Macrophage using
  immunocytochemistry (A and B)
. iPSC-Macrophages were cultured in Macrophage Maintenance
   Medium (ax0600) for 1 (A) and 4 (B) days post differentiation before staining for IBA1 and phalloidin.
   Phalloidin staining (red) confirmed the presence of F-actin filaments (B) and both images were
   counterstained with DAPI.

  • Flow Cytometry

   Flow cytometry for key cell surface markers of innate immunity. Axol iPSC-Derived Macrophages
   (ax0606) were harvested at day 7 using cold 1x PBS (containing 5 mM EDTA). The markers used were CD45
   (APC fluorophore, ImmunoTools), CD14 (PE fluorophore, ImmunoTools), CD11b (APC fluorophore, ImmunoTools).
   Axol macrophages are strongly positive for CD14, CD11b and CD45 . HLA-DR staining is minimal/negative as
   expected for unstimulated cells.

  • Phagocytosis inhibition assay

  Phagocytosis inhibition assay in Axol iPSC-Derived Macrophages. The macrophages were
   incubated with blue FluoSpheres and then treated with 5 μM CytoD or left untreated for control conditions.
   The macrophages were imaged live after 30, 60 and 90 mins. The DAPI beads can be seen in large vesicles 
   of the macrophages in the untreated culture. In comparison, the CytoD-treated culture shows smaller 
   macrophages with the DAPI beads in the medium. Data provided by Dr Will Stebbeds and Dr Graham Smith
   (Charles River Laboratories).

주문정보(Ordering Information)
ax0606Human iPSC-Derived Macrophages (Female)96-well plate
ax0601Human iPSC-Derived Macrophages (Male)96-well plate
ax0600Macrophage Maintenance Medium250 ml