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Endothelial colony forming cells (ECFCs)´Â °ñ¼ö ¹× Ç÷·ù¿¡¼ ¹ß°ßµÇ´Â ³»ÇǼ¼Æ÷·Î ¼Õ»óµÈ Ç÷°üÀÇ ¼ö¸®¸¦ ´ã´çÇÕ´Ï´Ù. AxolÀÇ iPSCÀ¯·¡ ECFCs´Â ³»ÇÇ Ç¥ÁöÀÚÀÎ CD31, CD144, KDR, NRP1À» ¹ßÇöÇÕ´Ï´Ù. |
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AXOL |
Human iPSC-derived endothelial colony forming cells | ||
Cat. No. | Product Name | Starting Material |
ax2019 | Human iPSC-Derived Endothelial Colony Forming Cells | Human iPS cells |
ax2219 | Human iPSC-Derived Endothelial Colony Forming Cell Kit | Human iPS cells |
Endothelial colony forming cell culture media and reagents | ||
Cat. No. | Product Name | Quantity |
ax2030-500 | Endothelial Colony Forming Cell Culture Medium | 500 mL |
ax0044 | Unlock | 25 mL |
ax0049 | Fibronectin Coating Solution | 1 mL |
Expression of the endothelial cell-specific markers: von Willebrand factor, endothelial NOS and CD31
in Axol Human iPSC-Derived Endothelial Colony Forming Cells (ECFCs). Hoechst 33342 was used to
visualize cell nuclei. The images were kindly provided by Dr Richard Siow (King's College London).
Axol iPSC-Derived Endothelial Colony Forming Cells (iPSC-ECFC) were seeded into a co-culture
vessel together with human lung fibroblasts. By day 4, the endothelial cells grow towards the lung
fibroblasts in response to secreted angiogenic factors. The process can be used to model the formation
of perfusable blood vessels during angiogenesis. The images were kindly provided by Dr Jungho Ahn
(Seoul National University).
Axol iPSC-Derived Endothelial Colony Forming Cells were co-cultured with adipose tissue-derived stem cells
in a fibrin clot (fibrinogen + thrombin). After 7 days, the cells have started to form a vascular network. The fibrin
clots were fixed and stained for CD31 (green). The images were kindly provided by Dr Wolfgang Holnthoner
(Ludwig-Boltzmann-Institute for Experimental and Clinical Traumatology).
Axol iPSC-Derived Endothelial Colony Forming Cells seeded into Matrigel
plugs form extensive 3D tubule networks after 24 hours in vitro.
Protocols
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