• Endothelial colony forming cells (ECFCs)는 골수 및 혈류에서 발견되는 내피세포로 손상된 혈관의 수리를 담당합니다.
• Axol의 iPSC유래 ECFCs는 내피 표지자인 CD31, CD144, KDR, NRP1을 발현합니다.
• 동일한 건강한 기증자의 일관된 내피세포를 이용하여 변동성을 최소화 하여 재현성있는 결과를 제공합니다.
• 2D, 3D 배양에서 모세관 구조를 형성하고 인간의 일차세포와 유사한 기능을 제공합니다.
• 항암효과 또는 증식 억제효과를 위한 화합물 스크리닝에 이상적입니다.
• Kit의 구성 :
  1) 1 vial of Endothelial Colony Forming Cells (1 million cells)
  2) 1 bottle of Endothelial Colony Forming Cell Culture Medium (500 mL)
  3) 1 vial of Fibronectin Coating Solution (1 mL) 
  4) 1 bottle of Unlock passaging reagent (25 mL)

Human iPSC-derived endothelial colony forming cells
Cat. No.	Product Name	Starting Material
ax2019	Human iPSC-Derived Endothelial Colony Forming Cells	Human iPS cells
ax2219	Human iPSC-Derived Endothelial Colony Forming Cell Kit	Human iPS cells

 

Endothelial colony forming cell culture media and reagents
Cat. No.	Product Name	Quantity
ax2030-500	Endothelial Colony Forming Cell Culture Medium	500 mL
ax0044	Unlock	25 mL
ax0049	Fibronectin Coating Solution	1 mL

• Endothelial cell marker expression

   Expression of the endothelial cell-specific markers: von Willebrand factor, endothelial NOS and CD31
   in Axol Human iPSC-Derived Endothelial Colony Forming Cells (ECFCs). Hoechst 33342 was used to
   visualize cell nuclei. The images were kindly provided by Dr Richard Siow (King's College London).

• Co-culture of iPSC-Derived Endothelial Colony Forming Cells with lung fibroblasts to assess angiogenic potential

   Axol iPSC-Derived Endothelial Colony Forming Cells  (iPSC-ECFC) were seeded into a co-culture
   vessel together with human lung fibroblasts. By day 4, the endothelial cells grow towards the lung
   fibroblasts in response to secreted angiogenic factors. The process can be used to model the formation
   of perfusable blood vessels during angiogenesis. The images were kindly provided by Dr Jungho Ahn
   (Seoul National University).

• Co-culture of iPSC-Derived Endothelial Colony Forming Cells with adipose tissue-derived stem cells to assess formation of vascular networks

   Axol iPSC-Derived Endothelial Colony Forming Cells were co-cultured with adipose tissue-derived stem cells
   in a fibrin clot (fibrinogen + thrombin). After 7 days, the cells have started to form a vascular network. The fibrin
   clots were fixed and stained for CD31 (green). The images were kindly provided by Dr Wolfgang Holnthoner
   (Ludwig-Boltzmann-Institute for Experimental and Clinical Traumatology).

• Angiogenesis

   Axol iPSC-Derived Endothelial Colony Forming Cells seeded into Matrigel
plugs form extensive 3D tubule networks after 24 hours in vitro. 

 


  Protocols