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 Á¦Ç°¾È³»  ¼¼Æ÷¹è¾ç   Stem Cell

hiPSC-Derived Endothelial Colony Forming Cells

Endothelial colony forming cells (ECFCs)´Â °ñ¼ö ¹× Ç÷·ù¿¡¼­ ¹ß°ßµÇ´Â ³»ÇǼ¼Æ÷·Î ¼Õ»óµÈ Ç÷°üÀÇ ¼ö¸®¸¦ ´ã´çÇÕ´Ï´Ù. AxolÀÇ iPSCÀ¯·¡ ECFCs´Â ³»ÇÇ Ç¥ÁöÀÚÀÎ CD31, CD144, KDR, NRP1À» ¹ßÇöÇÕ´Ï´Ù.

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  • Endothelial colony forming cells (ECFCs)´Â °ñ¼ö ¹× Ç÷·ù¿¡¼­ ¹ß°ßµÇ´Â ³»ÇǼ¼Æ÷·Î ¼Õ»óµÈ Ç÷°üÀÇ ¼ö¸®¸¦ ´ã´çÇÕ´Ï´Ù.
  • AxolÀÇ iPSCÀ¯·¡ ECFCs´Â ³»ÇÇ Ç¥ÁöÀÚÀÎ CD31, CD144, KDR, NRP1À» ¹ßÇöÇÕ´Ï´Ù.
  • µ¿ÀÏÇÑ °Ç°­ÇÑ ±âÁõÀÚÀÇ ÀϰüµÈ ³»ÇǼ¼Æ÷¸¦ ÀÌ¿ëÇÏ¿© º¯µ¿¼ºÀ» ÃÖ¼ÒÈ­ ÇÏ¿© ÀçÇö¼ºÀÖ´Â °á°ú¸¦ Á¦°øÇÕ´Ï´Ù.
  • 2D, 3D ¹è¾ç¿¡¼­ ¸ð¼¼°ü ±¸Á¶¸¦ Çü¼ºÇϰí Àΰ£ÀÇ ÀÏÂ÷¼¼Æ÷¿Í À¯»çÇÑ ±â´ÉÀ» Á¦°øÇÕ´Ï´Ù.
  • Ç×¾ÏÈ¿°ú ¶Ç´Â Áõ½Ä ¾ïÁ¦È¿°ú¸¦ À§ÇÑ È­ÇÕ¹° ½ºÅ©¸®´×¿¡ ÀÌ»óÀûÀÔ´Ï´Ù.
  • KitÀÇ ±¸¼º :
    1) 1 vial of Endothelial Colony Forming Cells (1 million cells)
    2) 1 bottle of Endothelial Colony Forming Cell Culture Medium (500 mL)
    3) 1 vial of Fibronectin Coating Solution (1 mL) 
    4) 1 bottle of Unlock passaging reagent (25 mL)





 Human iPSC-derived endothelial colony forming cells 
 Cat. No. Product Name   Starting Material
 ax2019 Human iPSC-Derived Endothelial Colony Forming Cells    Human iPS cells
 ax2219 Human iPSC-Derived Endothelial Colony Forming Cell Kit    Human iPS cells

 

 Endothelial colony forming cell culture media and reagents 
 Cat. No. Product Name  Quantity
 ax2030-500 Endothelial Colony Forming Cell Culture Medium  500 mL
 ax0044 Unlock  25 mL
 ax0049 Fibronectin Coating Solution  1 mL






  • Endothelial cell marker expression




   Expression of the endothelial cell-specific markers: von Willebrand factor, endothelial NOS and CD31
   in Axol Human iPSC-Derived Endothelial Colony Forming Cells (ECFCs). Hoechst 33342 was used to
   visualize cell nuclei. The images were kindly provided by Dr Richard Siow (King's College London).


  • Co-culture of iPSC-Derived Endothelial Colony Forming Cells with lung fibroblasts to assess angiogenic potential



   Axol iPSC-Derived Endothelial Colony Forming Cells  (iPSC-ECFC) were seeded into a co-culture
   vessel together with human lung fibroblasts. By day 4, the endothelial cells grow towards the lung
   fibroblasts in response to secreted angiogenic factors. The process can be used to model the formation
   of perfusable blood vessels during angiogenesis. The images were kindly provided by Dr Jungho Ahn
   (Seoul National University).


  • Co-culture of iPSC-Derived Endothelial Colony Forming Cells with adipose tissue-derived stem cells to assess formation of vascular networks



   Axol iPSC-Derived Endothelial Colony Forming Cells were co-cultured with adipose tissue-derived stem cells
   in a fibrin clot (fibrinogen + thrombin). After 7 days, the cells have started to form a vascular network. The fibrin
   clots were fixed and stained for CD31 (green). The images were kindly provided by Dr Wolfgang Holnthoner
   (Ludwig-Boltzmann-Institute for Experimental and Clinical Traumatology).


  • Angiogenesis



   Axol iPSC-Derived Endothelial Colony Forming Cells seeded into Matrigel
plugs form extensive 3D tubule networks after 24 hours in vitro


 


  Protocols

 
 
ÁÖ¹®Á¤º¸(Ordering Information)
CATNUM PRODUCT SIZE
ax2030-500Endothelial Colony Forming Cell Culture Medium500 mL
ax0049Fibronectin Coating Solution1 mL
ax2219Human iPSC-Derived Endothelial Colony Forming Cell Kit1 vial
ax2019Human iPSC-Derived Endothelial Colony Forming Cells1 vial
ax0044Unlock25 mL