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Axol iPSC 유래 Renal Proximal Tubular Cells는 상피 세포 및 신장 세포 마커와 함께 중요한 기능적 마커를 발현합니다.
|Human iPSC-derived renal proximal tubular cells|
|Cat. No.||Product Name||Starting Material|
|ax2115||Human iPSC-derived Renal Proximal Tubular Cells (Male)||Cord Blood CD34+ Cells|
|Renal epithelial cell culture media|
|Cat. No.||Product Name||Quantity|
|ax3534||Renal Epithelial Cell Culture Media||500ml|
Phase contrast images of Axol Human iPSC-Derived Renal Proximal Tubular Cells at day
4 post-thaw. Human iPSC-derived Renal Proximal Tubular Cells are seeded using Renal Epithelial Cell Culture
Medium at a density of 50,000 cells/cm2. The iPSC-derived renal proximal tubular cells form an adherent,
confluent monolayer with typical epithelial morphology by day 4 after seeding.
Expression of the ZO-1, URO10, Na+/K+ ATPase and GLUT1 proteins were confirmed in
Axol iPSC-Derived Renal Proximal Tubular Cells using immunocytochemistry (A, B, C and D).
Staining for ZO-1, URO10 and Na+/K+ ATPase (red) and GLUT1 (green) respectively confirmed the presence
of tight junctions (A), urothelial glycoprotein (present on the surface of proximal tubule cells) (B),
sodium-potassium pumps (C) and the uniporter protein, glucose transporter 1 (D). DAPI counterstain.
Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) analysis was used to
determine the gene expression levels of 22 genes that identify renal proximal tubular cells.
RT-qPCR reveals the expression of 22 genes important for proximal tubular cell function.
Key transporter genes that are not highly expressed in human primary renal proximal tubular cells such as
SLC34A1 and SGLT2 are expressed in iPSC-Derived Renal Proximal Tubular Cells. The expression of these
genes supports the use of Axol iPSC-Derived Renal Proximal Tubular Cells in transportation assays and in the
detection and prediction of nephrotoxicity.
Albumin endocytosis in iPSC-Derived Renal Proximal Tubular Cells
iPSC-derived renal proximal tubular cells, primary human renal proximal tubular cells and human
kidney tumor cells were treated with 100 µg/mL FITC-conjugated BSA in serum-free DMEM-F12 for
16 hours, in parallel. The FITC-BSA can be seen in the cytoplasm of each cell type signifying that the
renal proximal tubular cells are able to endocytose albumin.
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