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 제품안내  세포배양   Stem Cell

hiPSC-Derived Renal Proximal Tubular Cells

Axol iPSC 유래 Renal Proximal Tubular Cells는 상피 세포 및 신장 세포 마커와 함께 중요한 기능적 마커를 발현합니다.

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  • Axol iPSC 유래 Renal Proximal Tubular Cells는 상피 세포 및 신장 세포 마커와 함께 중요한 기능적 마커를 발현합니다.
  • Renal Epithelial Cell Culture Media는 신장근위 관상세포의 최적의 배양을 위한 호르몬과 성장인자가 포함되어 있습니다.
  • epithelial, renal cell marker와 함께 기능적인 marker도 발현됩니다. (SLC34A1, OAT1, SGLT2 등)
  • 당뇨병에서 glucose uptate를 조사하기 위한 모델로 적합하며, drug의 운송, 내부 대사, 신장독성 확인 등에도 유용하게 이용될 수 있습니다.          


 Human iPSC-derived renal proximal tubular cells 
 Cat. No. Product Name   Starting Material
 ax2115 Human iPSC-derived Renal Proximal Tubular Cells (Male)   Cord Blood CD34+ Cells

 

 Renal epithelial cell culture media 
 Cat. No. Product Name Quantity
 ax3534 Renal Epithelial Cell Culture Media 500ml






  • Phase Contrast




   Phase contrast images of Axol Human iPSC-Derived Renal Proximal Tubular Cells
at day
   4 post-thaw. Human iPSC-derived Renal Proximal Tubular Cells are seeded using Renal Epithelial Cell Culture
   Medium at a density of 50,000 cells/cm2. The iPSC-derived renal proximal tubular cells form an adherent,
   confluent monolayer with typical epithelial morphology by day 4 after seeding.

 

  • Immunocytochemistry




   Expression of the ZO-1, URO10, Na+/K+ ATPase and GLUT1 proteins were confirmed in
   Axol iPSC-Derived Renal Proximal Tubular Cells using immunocytochemistry (A, B, C and D)
.
    Staining for ZO-1, URO10 and Na+/K+ ATPase (red) and GLUT1 (green) respectively confirmed the presence
    of tight junctions (A), urothelial glycoprotein (present on the surface of proximal tubule cells) (B),
    sodium-potassium pumps (C) and the uniporter protein, glucose transporter 1 (D). DAPI counterstain.


  • Gene Expression




     Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) analysis was used to
     determine the gene expression levels of 22 genes that identify renal proximal tubular cells.

    RT-qPCR reveals the expression of 22 genes important for proximal tubular cell function. 
     Key transporter genes that are not highly expressed in human primary renal proximal tubular cells such as
    SLC34A1 and SGLT2 are expressed in iPSC-Derived Renal Proximal Tubular Cells. The expression of these
    genes supports the use of Axol iPSC-Derived Renal Proximal Tubular Cells in transportation assays and in the
    detection and prediction of nephrotoxicity.



  • Albumin Endocytosis


Albumin endocytosis in iPSC-Derived Renal Proximal Tubular Cells



   iPSC-derived renal proximal tubular cells, primary human renal proximal tubular cells and human
   kidney tumor cells were treated with 100 µg/mL FITC-conjugated BSA in serum-free DMEM-F12 for
   16 hours, in parallel. The FITC-BSA can be seen in the cytoplasm of each cell type signifying that the
   renal proximal tubular cells are able to endocytose albumin.




  Protocol

주문정보(Ordering Information)
CATNUM PRODUCT SIZE
ax2115Human iPSC-derived Renal Proximal Tubular Cells (Male)1 vial
ax3534Renal Epithelial Cell Culture Media500ml