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 Á¦Ç°¾È³»  ¼¼Æ÷¹è¾ç   Stem Cell

hiPSC-Derived Striatal Neurons

striatum (¼±Á¶Ã¼)´Â µ¿±âºÎ¿©, ¿îµ¿ °èȹ µîÀ» Æ÷ÇÔÇÏ¿© ´Ù¾çÇÑ µÎ³ú ±â´ÉÀ» Á¶Á¤ÇÏ´Â ¿ªÇÒÀ» ÇÕ´Ï´Ù.

AxolÀÇ striatal neuron medium kit´Â Huntington's disease patient-derived NSCs (ax0211)°ú ÇÔ²² »ç¿ëÇÏ¿©, Áúº´°ü·Ã ¼¼Æ÷ À¯Çü (MSNs)°ú ½ÇÁ¦ ȯÀÚ À¯Àüü ¹è°æ (50GAC repeat)À¸·Î in vitro ÇåÆÃÅÏ Áúȯ ¸ðµ¨À» ±¸ÃàÇÒ ¼ö ÀÖ½À´Ï´Ù.

AXOL
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  • striatum (¼±Á¶Ã¼)´Â µ¿±âºÎ¿©, ¿îµ¿ °èȹ µîÀ» Æ÷ÇÔÇÏ¿© ´Ù¾çÇÑ µÎ³ú ±â´ÉÀ» Á¶Á¤ÇÏ´Â ¿ªÇÒÀ» ÇÕ´Ï´Ù.
  • AxolÀÇ striatal neuron medium kit´Â Huntington's disease patient-derived NSCs (ax0211)°ú ÇÔ²² »ç¿ëÇÏ¿©, Áúº´°ü·Ã ¼¼Æ÷ À¯Çü (MSNs)°ú ½ÇÁ¦ ȯÀÚ À¯Àüü ¹è°æ (50GAC repeat)À¸·Î in vitro ÇåÆÃÅÏ Áúȯ ¸ðµ¨À» ±¸ÃàÇÒ ¼ö ÀÖ½À´Ï´Ù.
  • ´ëºÎºÐÀÇ human striatal neuronÀº γ-amino butyric acid (GABAergic) projection medium spiny neurons (MSNs) À̸ç, ÀÌ ¼¼Æ÷µéÀº dopamine- and cAMP-regulated phosphoprotein - DARPP32¸¦ ¹ßÇöÇÕ´Ï´Ù.
  • Striatal neuronÀº Huntington’s disease (HD, ÇåÆÃÅϺ´)ÀÇ Ãʱ⠴ܰ迡¼­ ƯÈ÷ ÅðÇàÇÏ¿©, ȯÀÚÀÇ ¿îµ¿ ¹× ÀÎÁö ±â´ÉÀ» ¼Õ»ó½Ãŵ´Ï´Ù.
  • Axol»çÀÇ striatal neuron medium kitÀº ½Å°æÁٱ⼼Æ÷¸¦ Striatal neuronal lineage·Î À¯µµÇÕ´Ï´Ù.
  • Striatal Neuron Medium Kit ±¸¼º :
    1) 500 mL basal medium
    2) 15 mL supplement
    3) 1 vial of lyophilized supplement





 Striatal Neuron culture media and reagents
 Cat. No. Product Name Quantity
 ax0333 Striatal Neuron Medium Kit (500 mL basal medium; 15 mL supplement; 1 vial of lyophilized supplement) 1 Kit
 ax0052  SureBond + ReadySet (For coating culture vessel)   1 Kit
 ax139800    Human Brain-derived Neurotrophic Factor (BDNF)     10 µg / 100 μg
 ax139855    Human Glia-derived Neurotrophic Factor (GDNF)    10 µg / 100 μg
 ax68168 Y-27632 2HCl (ROCK Inhibitor)  5 mg


  • Phase contrast images of differentiating striatal neural culture



   Phase contrast images of Axol Human iPSC-derived Striatal Neurons from 1 day post thawing.
   
Ax0015 NSCs were seeded at 10,000 cells per cm2 (relatively low density for later ICC imaging purpose).
   These images provide an overview of the health status during striatal differentiation. All images were acquired
   using the EVOS microscopy using 10X magnification.

  • Validation of key marker expression by immunocytochemistry


    ICC images of differentiating striatal neural culture. Cells were fixed and stained on day 10, day 18 and day 33
    for markers of striatal neurons (medium spiny neurons): DARPP32, CTIP2, CALBINDIN, GABA and MAP2





 

 

ÁÖ¹®Á¤º¸(Ordering Information)
CATNUM PRODUCT SIZE
ax139800BDNF10 ¥ìg
ax139855GDNF10ug
ax0333Striatal Neuron Medium Kit1 Kit
ax0052SureBond + ReadySet (For coating culture vessel) 1 Kit
ax68168Y-27632 2HCl (ROCK Inhibitor)5 mg