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 Á¦Ç°¾È³»  ¼¼Æ÷¹è¾ç   Stem Cell

hiPSC-Derived Astrocytes

Human iPSC¿¡¼­ À¯·¡ µÈ Astrocyte (¼º»ó±³¼¼Æ÷)·Î, ¾ËÃ÷ÇÏÀÌ¸Ó ¹× ÆÄŲ½¼ º´, ÀÚÆóÁõ, ±Ù À§Ã༺ Ãø»è °æÈ­Áõ (ALS), ·¹Æ® ÁõÈıº ¹× Á¶Çöº´°°Àº ¸¹Àº ½Å°æÇÐÀû Áõ»ó ¿¬±¸¿¡ ÀÌ¿ëµË´Ï´Ù.

AXOL
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  • ¼º»ó±³¼¼Æ÷´Â ¾ËÃ÷ÇÏÀÌ¸Ó ¹× ÆÄŲ½¼ º´, ÀÚÆóÁõ, ±Ù À§Ã༺ Ãø»è °æÈ­Áõ (ALS), ·¹Æ® ÁõÈıº ¹× Á¶Çöº´ °°Àº ¸¹Àº ½Å°æÇÐÀû Áõ»ó ¿¬±¸¿¡ °ü·ÃÀÌ ÀÖ½À´Ï´Ù.
  • Progenitor KitÀº »ç¿ëÀÌ Æí¸®ÇÑ culture systemÀ¸·Î Á¦°øµÇ¸ç, ½Å¾à°³¹ß ¹× Áúº´ ¸ðµ¨¸µÀ» À§ÇØ Àå±â°£ ¼¼Æ÷°¡ »ýÁ¸ÇÒ ¼ö ÀÖ´Â ´É·ÂÀ» ÃËÁø½ÃÄ×½À´Ï´Ù.
  • iPSC-Derived Astrocyte´Â Àΰ£ÀÇ ÀÏÂ÷ ¼º»ó±³¼¼Æ÷¿Í ±â´ÉÀÌ µ¿µîÇÏ¿© in vitro ¿¡¼­ º¸´Ù Á¤È®ÇÑ Astrocyte ¿¬±¸¸¦ µµ¸ðÇÒ ¼ö ÀÖ½À´Ï´Ù.
¡¡ iPSC-Derived Astrocyte Progenitor Kit
  • 3ÁÖ À̳»¿¡ ¼º¼÷ÇÑ Astrocyte¸¦ »ý¼ºÇϸç, GFAP, S100b¿Í °°Àº Ư¡Àû ¸¶Ä¿¸¦ ¹ßÇöÇÕ´Ï´Ù. 
  • ¼¼Æ÷ÀÇ ±¸¼º :
    1) 80%´Â GFAP+ (Glial Fibrillary Acidic Protein)
    2) ³ª¸ÓÁö ≤15%´Â TUJ1+ (neuronal class III ß-tubulin) neurons
  • Kit ±¸¼º :
    1) 1 vial of iPSC-Derived Astrocyte Progenitors (≥1 million cells)
    2) 1 x 100 mL bottle of Astrocyte Maintenance Medium (Supplement A, B, C Æ÷ÇÔ)
¡¡ iPSC-Derived Astrocyte (ax0665) NEW
  • Àΰ£ÀÇ ÀÏÂ÷ ¼º»ó±³¼¼Æ÷¿Í ±â´ÉÀÌ µ¿µîÇÑ iPSC-Derived Astrocyte ÀÔ´Ï´Ù.
  • ³Ãµ¿º¸°üµÇ¸ç, ÀÏ´Ü ÇØµ¿µÇ¸é 48½Ã°£ ³»¿¡ ¸ðµç Astrocyte-specific marker¸¦ ¹ßÇöÇϰí 48-60 ½Ã°£ ³»¿¡ ÁÖ¿ä ±â´ÉÀû Ư¼ºÀÎ Glutamate Àڱؿ¡ ¹ÝÀÀÇÕ´Ï´Ù.
  • ´Üµ¶À¸·Î, ȤÀº ÁßÃ߽Űæ°è¿¡ Á¸ÀçÇÏ´Â ´Ù¸¥ ¼¼Æ÷¿ÍÀÇ co-culture°¡ °¡´ÉÇÕ´Ï´Ù.
  • GFAP, S100b, AQP4, ALDHL1L ¹× EAAT1°ú °°Àº ÇÁ·ÎÅä ŸÀÔ Ç¥ÇöÇü ¸¶Ä¿¸¦ ¹ßÇöÇÕ´Ï´Ù.
  • Vimentin, Kir4.1 (Ä®·ý ä³Î) ¹× CD44¸¦ ºñ·ÔÇÑ ´Ù¸¥ ÁÖ¿ä biomarkerµµ ¹ßÇöÇÕ´Ï´Ù.
  • Cell number : > 0.5 million cells/vial
  • ax0086 (Astrocyte maintenance medium)°ú ÇÔ²² »ç¿ëÇÕ´Ï´Ù.




 iPSC-derived astrocytes
 Cat. No. Product Name Donor
 ax0083 Human iPSC-Derived Astrocyte Progenitor Kit Male, newborn
 ax0084 Human iPSC-Derived Mature Astrocyte Kit Male, newborn
 ax0081 Human iPSC-Derived Astrocyte Progenitor Kit Female, newborn
 ax0082 Human iPSC-Derived Mature Astrocyte Kit Female, newborn
 ax0665 Human iPSC-Derived Astrocytes Male, newborn

 

 Astrocyte culture media and reagents
 Cat. No. Product Name Quantity
 ax0086 Astrocyte Maintenance Medium  100 mL (basal medium, pluc suplements)



Àû¿ë
  • Immunocytochemistry¸¦ ÀÌ¿ëÇÑ ¸¶Ä¿ ¹ßÇöÀÇ ºñ±³



   Axol iPSC-derived Mature Astrocytes express astrocyte-specific markers GFAP and S100B.
    Low expression of nestin, a marker of progenitor cell phenotype and MAP2, a marker of microtubule growth
    and neuritogenesis, indicates successful glial differentiation of the iPSCs. Cells seeded on Matrigel at
    16,000 cells/well of a 96-well plate. Data provided Susanne Zach, Boehringer Ingelheim.



    Axol iPSC-derived Mature Astrocytes express astrocyte associated markers excitatory amino 
    acid transporter 1 (EAAT1), glutamine synthetase (GS) and ALDH1L1
. EAAT1 is active in clearly
     excitatory glutamate from the extracellular space, preventing neurotoxicity where GS converts glutamate to
     glutamine. The expression of these markers highlights that Axol’s Human iPSC-derived Astrocytes are
     physiologically representative of human primary astrocytes. Cells seeded on Matrigel at 16,000 cells/well
     of a 96-well plate. Data provided Susanne Zach, Boehringer Ingelheim.

  • Calcium Activity

  • Astrocyte-specific markers expression (ax0665)

 

  • WHITE matter¿¡ ¿ì¼±ÇÑ grey+white matter mixture Astrocyte (ax0665)



  • MEA¿¡ ÀÇÇÑ Astrocyte(ax0665)ÀÇ Æ¯¼º ÀÔÁõ








  Protocols

ÁÖ¹®Á¤º¸(Ordering Information)
CATNUM PRODUCT SIZE
ax0086Astrocyte Maintenance Medium 100 mL
ax0083Human iPSC-Derived Astrocyte Progenitor Kit1 Kit
ax0081Human iPSC-Derived Astrocyte Progenitor Kit1 Kit
ax0665Human iPSC-Derived Astrocytes1 vial
ax0084Human iPSC-Derived Mature Astrocyte Kit1 Kit
ax0082Human iPSC-Derived Mature Astrocyte Kit1 Kit