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 Á¦Ç°¾È³»  ¼¼Æ÷¹è¾ç   Stem Cell

hiPSC-Derived Motor Neuron Progenitors

hiPSC·Î À¯µµµÈ Motor neuron progenitor¸¦ ¼Ò°³ÇÕ´Ï´Ù.

AXOL
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  • Progenitor cellÀº Motor neuron cell fate markers (OLIG2°ú SOX1)¸¦ ¹ßÇöÇÕ´Ï´Ù.
  • Mature stage¿¡¼­´Â Motor neuronÀÇ ´ëÇ¥ÀûÀÎ ¸¶Ä¿ÀÎ HB9, LIM3, ChAT¸¦ ¹ßÇöÇÕ´Ï´Ù.
  • ¹è¾ç¿¡ ÇÊ¿äÇÑ media¸¦ Á¦°øÇÕ´Ï´Ù. ƯÈ÷ Recovery media´Â Àü±¸¼¼Æ÷°¡ iPSC-derived motor neuronsÀ¸·Î ¼ºÀå, ¼º¼÷Çϴµ¥ ÃÖÀûÀÇ Á¶°ÇÀ» Á¦°øÇÕ´Ï´Ù.
  • µ¿¹°¸ðµ¨À» ´ë½ÅÇÏ¿© drug-screening assay, ¿îµ¿½Å°æ¼¼Æ÷ ÅðÇ༺ ¸ðµ¨µîÀÇ ¿¬±¸¿¡ ÀÌ¿ëµË´Ï´Ù.
  • ¼¼Æ÷ Á¦°ø·® : ≥2 x 106 cells



 Human iPSC-derived motor neuron progenitors 
 Cat. No. Product Name Donor
 ax0078 Motor Neuron Progenitors (Healthy) Fibroblasts (74 yr Male)
 ax0073 Motor Neuron Progenitors (Healthy - sibling (C9ORF72 extension))  Fibroblasts (62 yr Male)
 ax0074 Motor Neuron Progenitors (ALS (C9ORF72 extension))  Fibroblasts (64 yr Female)
 ax0178 Human iPSC-Derived Motor Neuron Progenitor Kit (Male) Fibroblasts (74 yr Male)


 Motor neuron culture media and reagents 
 Cat. No. Product Name Quantity
 ax0071 Motor Neuron Recovery Medium  50 mL
 ax0072 Motor Neuron Maintenance Medium  200 mL
 ax0041 SureBond  3 vials of SureBond (120 μL/vial)
 ax0052 SureBond+ReadySet  3 vials of SureBond (120 μL/vial) and 2 vials of ReadySet (10 mL/vial)
 ax0044 Unlock  25 mL



Àû¿ë
  • Mature motor neuronÀÇ ¸¶Ä¿ HB9, LIM3 and ChATÀÇ ¹ßÇö (Immunocytochemistry)    

           Protein expression of HB9, LIM3 and ChAT was confirmed in Axol iPSC-derived motor neuron
           using immunocytochemistry. Human iPSC-motor neurons were cultured in motor neuron
           maintenance medium (ax0072) for 14 days post passaging before staining for motor neuron 
           markers LIM3 (green), HB9 (red) and ChAT (red) along with staining for neural markers 
          TUJ1 (green) and MAP2 (red). Merged images were counterstained with Hoechst.

  • Voltage Clamp




     (Ai) Synaptic activity recorded from a Human iPSC-derived Motor Neuron using whole-cell patch-clamp
     technique (voltage clamp mode). (Aii) Synaptic activity shown on a faster time scale to reveal the kinetics
     of individual postsynaptic currents. (B) Traces showing fast, inactivating Na+ currents recorded in response
     to brief depolarizing voltage steps. (C) Traces showing persistent K+ currents recorded in response to longer
     duration depolarizing voltage steps. Data kindly provided by Amit K. Chouhan and Dr Gareth Miles
     (University of St Andrews).





  Protocol        

ÁÖ¹®Á¤º¸(Ordering Information)
CATNUM PRODUCT SIZE
ax0178Human iPSC-Derived Motor Neuron Progenitor Kit (Male)1 vial
ax0072Motor Neuron Maintenance Medium 200 mL
ax0074Motor Neuron Progenitors (ALS (C9ORF72 extension)) 1 vial
ax0073Motor Neuron Progenitors (Healthy - sibling (C9ORF72 extension)) 1 vial
ax0078Motor Neuron Progenitors (Healthy)1 vial
ax0071Motor Neuron Recovery Medium 50 mL
ax0041SureBond 3 vials
ax0052SureBond+ReadySet 3 vials and 2 vials
ax0044Unlock25 mL