• Mature motor neuron의 마커 HB9, LIM3 and ChAT의 발현 (Immunocytochemistry)    
  
  

           Protein expression of HB9, LIM3 and ChAT was confirmed in Axol iPSC-derived motor neuron
           using immunocytochemistry. Human iPSC-motor neurons were cultured in motor neuron
           maintenance medium (ax0072) for 14 days post passaging before staining for motor neuron 
           markers LIM3 (green), HB9 (red) and ChAT (red) along with staining for neural markers 
          TUJ1 (green) and MAP2 (red). Merged images were counterstained with Hoechst.

• Voltage Clamp

     (Ai) Synaptic activity recorded from a Human iPSC-derived Motor Neuron using whole-cell patch-clamp
     technique (voltage clamp mode). (Aii) Synaptic activity shown on a faster time scale to reveal the kinetics
     of individual postsynaptic currents. (B) Traces showing fast, inactivating Na+ currents recorded in response
     to brief depolarizing voltage steps. (C) Traces showing persistent K+ currents recorded in response to longer
     duration depolarizing voltage steps. Data kindly provided by Amit K. Chouhan and Dr Gareth Miles
     (University of St Andrews).

  Protocol