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hiPSC·Î À¯µµµÈ Motor neuron progenitor¸¦ ¼Ò°³ÇÕ´Ï´Ù. |
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AXOL |
Human iPSC-derived motor neuron progenitors | ||
Cat. No. | Product Name | Donor |
ax0078 | Motor Neuron Progenitors (Healthy) | Fibroblasts (74 yr Male) |
ax0073 | Motor Neuron Progenitors (Healthy - sibling (C9ORF72 extension)) | Fibroblasts (62 yr Male) |
ax0074 | Motor Neuron Progenitors (ALS (C9ORF72 extension)) | Fibroblasts (64 yr Female) |
ax0178 | Human iPSC-Derived Motor Neuron Progenitor Kit (Male) | Fibroblasts (74 yr Male) |
Motor neuron culture media and reagents | ||
Cat. No. | Product Name | Quantity |
ax0071 | Motor Neuron Recovery Medium | 50 mL |
ax0072 | Motor Neuron Maintenance Medium | 200 mL |
ax0041 | SureBond | 3 vials of SureBond (120 μL/vial) |
ax0052 | SureBond+ReadySet | 3 vials of SureBond (120 μL/vial) and 2 vials of ReadySet (10 mL/vial) |
ax0044 | Unlock | 25 mL |
Protein expression of HB9, LIM3 and ChAT was confirmed in Axol iPSC-derived motor neuron
using immunocytochemistry. Human iPSC-motor neurons were cultured in motor neuron
maintenance medium (ax0072) for 14 days post passaging before staining for motor neuron
markers LIM3 (green), HB9 (red) and ChAT (red) along with staining for neural markers
TUJ1 (green) and MAP2 (red). Merged images were counterstained with Hoechst.
(Ai) Synaptic activity recorded from a Human iPSC-derived Motor Neuron using whole-cell patch-clamp
technique (voltage clamp mode). (Aii) Synaptic activity shown on a faster time scale to reveal the kinetics
of individual postsynaptic currents. (B) Traces showing fast, inactivating Na+ currents recorded in response
to brief depolarizing voltage steps. (C) Traces showing persistent K+ currents recorded in response to longer
duration depolarizing voltage steps. Data kindly provided by Amit K. Chouhan and Dr Gareth Miles
(University of St Andrews).
Protocol
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