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 제품안내  형광시약   Protein Labeling

Janelia Fluor® Dyes

bright, photostable, cell permeable dye를 소개합니다.

TOCRIS
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특징
  • 세포막을 통과하는 photostable dye입니다.
  • NHS ester로 제공되어 amine group과 결합이 가능합니다.



 Product JF 549 JF 585 JF 635 JF 646 JF 669 JF525 PA JF549 PA JF646
 Cat. No 6147 6418 6419 6148 6420 6296 6149 6150
 Emission
 color
Yellow Orange Red Red Far-Red Yellow Yellow Red
 labs (nm) 549 585 635 646 669 525 551 - 553 649 - 651
 lem (nm) 571 609 652 664 682 549 570 - 573 663 - 665
 Extinction   coefficient
 (e, M-1cm-1)
101000 156000 167000 152000 116000 122,000 - -
 Quantum 
 yield (f)
0.88 0.78 0.56 0.54 0.37 0.91 - -
 Key
 Features/
 Applications
Suitable for confocal and super resolution imaging techniques Good two-photon fluorescence at 1,100 nm excitation Low background staining. Used in vivo as a HaloTag® conjugate Suitable for confocal and super resolution imaging techniques Exceptionally photostable far-red dye Confocal fluorescent imaging and super resolution microscopy (SRM)  Single molecule tracking and super resolution microscopy in live cells Single molecule tracking and super resolution microscopy in live cells












  • Grimm et al. (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 12, 244. PMID: 25599551
  • Grimm et al. (2016) Bright photoactivatable fluorophores for single molecule imaging. Nat.Methods 13, 285. PMID: 27776112
  • Grimm et al. (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14, 987. PMID: 28869757
  • Grimm et al. (2017) A general method for Si-Fluoresceins and Si-Rhodamines. ACS Cent Sci 3, 975. PMID: 28979939
  • Lavis (2017) Chemistry is Dead. Long Live Chemistry. Biochemistry 56, 5165. PMID: 28704030
  • Tsai et al. (2017) Nuclear microenvironments modulate transcription from low-affinity enhancers. ELife 6, e28975. PMID: 29095143
  • Yadav & Chan (2017) Bright dyes bring biology into focus. ACS Cent Sci 9, 920. PMID: 28979930
  • Zhang et al. (2017) Optogenetic control of kinetochore function. Nat.Chem.Biol 13, 1096. PMID: 28805800