Janelia Fluor® Dyes


bright, photostable, cell permeable dye를 소개합니다.
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Product	JF 549	JF 585	JF 635	JF 646	JF 669	JF525	PA JF549	PA JF646
Cat. No	6147	6418	6419	6148	6420	6296	6149	6150
Emission color	Yellow	Orange	Red	Red	Far-Red	Yellow	Yellow	Red
labs (nm)	549	585	635	646	669	525	551 - 553	649 - 651
lem (nm)	571	609	652	664	682	549	570 - 573	663 - 665
Extinction coefficient (e, M-1cm-1)	101000	156000	167000	152000	116000	122,000	-	-
Quantum yield (f)	0.88	0.78	0.56	0.54	0.37	0.91	-	-
Key Features/ Applications	Suitable for confocal and super resolution imaging techniques	Good two-photon fluorescence at 1,100 nm excitation	Low background staining. Used in vivo as a HaloTag® conjugate	Suitable for confocal and super resolution imaging techniques	Exceptionally photostable far-red dye	Confocal fluorescent imaging and super resolution microscopy (SRM)	Single molecule tracking and super resolution microscopy in live cells	Single molecule tracking and super resolution microscopy in live cells

Grimm et al. (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 12, 244. PMID: 25599551
Grimm et al. (2016) Bright photoactivatable fluorophores for single molecule imaging. Nat.Methods 13, 285. PMID: 27776112
Grimm et al. (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14, 987. PMID: 28869757
Grimm et al. (2017) A general method for Si-Fluoresceins and Si-Rhodamines. ACS Cent Sci 3, 975. PMID: 28979939
Lavis (2017) Chemistry is Dead. Long Live Chemistry. Biochemistry 56, 5165. PMID: 28704030
Tsai et al. (2017) Nuclear microenvironments modulate transcription from low-affinity enhancers. ELife 6, e28975. PMID: 29095143
Yadav & Chan (2017) Bright dyes bring biology into focus. ACS Cent Sci 9, 920. PMID: 28979930
Zhang et al. (2017) Optogenetic control of kinetochore function. Nat.Chem.Biol 13, 1096. PMID: 28805800

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