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AKTA FPLC Àåºñ¿¡ »ç¿ëÇÒ¼ö ÀÖ´Â columnÀ¸·Î Ion exchange resinÀÌ ÃæÀüµÇ¾î ÀÖ´Â column ÀÔ´Ï´Ù. IEX SP (cation exchange chromatography)¿Í IEX Q (anion exchange chromatography) °¡ ÀÖ½À´Ï´Ù. |
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Protein Ark |
Adsorbent | IEX SP-Resin | IEX Q-Resin |
Ligand | Sulphonic acid with a propyl linker | Quaternary ammonium |
Type of ion exchanger | Strong cation | Strong anion |
Total ionic capacity | ≥ 175 μmol/g settled gel | 40-60μmol/g |
Mean particle size | 90± 10μm | |
Matrix | 6% cross-linked near monodisperse agarose | Highly cross-linked 6% near monodisperse agarose |
Binding capacity | ≥ 90 mg/mL of adsorbent (lysozyme) | 75 - 100 mg/g of adsorbent (BSA) |
Recommended packing conditions | At a constant pressure of 1.5 bar (~ 22 psi) | |
Recommended packing solutions | ¡¡ | 0.1 M NaCl solution or equilibration buffer |
Recommended operational flow rates | Up to 600 cm/h | |
Operating pH | pH4.0– pH14.0 | pH2.0– pH14.0 |
Chemical stability | All commonly used aqueous buffers and co-solvents | |
Cleaning/sanitization | 0.5 to 1.0 M NaOH | |
Sterilization | Autoclavable in 0.1M NaCl solution at 121°C for 30 minutes | |
Storage conditions | 2 – 30°C , 20% ethanol, 0.2M sodium acetate | 2 – 30°C , 20% ethanol |
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