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 Á¦Ç°¾È³»  ºÐÀÚ»ý¹°ÇР  Protein Research  Protein concentration & Extraction

HiFliQ FPLC Ion Exchange Columns

AKTA FPLC Àåºñ¿¡ »ç¿ëÇÒ¼ö ÀÖ´Â columnÀ¸·Î Ion exchange resinÀÌ ÃæÀüµÇ¾î ÀÖ´Â column ÀÔ´Ï´Ù. IEX SP (cation exchange chromatography)¿Í IEX Q (anion exchange chromatography) °¡ ÀÖ½À´Ï´Ù.

Protein Ark
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  • resinÀÇ ÀÔÀÚ Å©±â°¡ ±ÕÀÏÇϰí packing ÀÌ ¼Õ½±½À´Ï´Ù.
  • Excellent flow properties
  • ºÐÇØ´ÉÀÌ ¶Ù¾î³ª¸ç, binding capacities °¡ ³ô½À´Ï´Ù.
  • Cost effective & reliable
  • Easy to clean (robust, long life adsorbents)
  • ¹«¿ë¸Å °øÁ¤À¸·Î »ý»êµÈ resinÀ» »ç¿ëÇÏ¿©, ºÐ¸®µÈ proteinÀÇ ¼Õ»óÀÌ Àû½À´Ï´Ù.



  • SP IEX HiFlIQ : sulphonic acid ±×·ìÀÇ ±³È¯±â°¡ ÈíÂøµÈ °í¼º´ÉÀÇ °­·ÂÇÑ ¾çÀ̿ Ä÷³ ÀÔ´Ï´Ù.
  • Q  IEX HiFlIQ : quaternary ammonium ±×·ìÀÇ ±³È¯±â°¡ ÈíÂøµÈ °í¼º´ÉÀÇ °­·ÂÇÑ À½À̿ Ä÷³ ÀÔ´Ï´Ù.



Adsorbent IEX SP-Resin  IEX Q-Resin 
Ligand Sulphonic acid with a propyl linker   Quaternary ammonium  
Type of ion exchanger Strong cation   Strong anion  
Total ionic capacity ≥ 175 μmol/g settled gel   40-60μmol/g
Mean particle size 90± 10μm 
Matrix 6% cross-linked near monodisperse agarose  Highly cross-linked 6% near monodisperse agarose
Binding capacity ≥ 90 mg/mL of adsorbent (lysozyme) 75 - 100 mg/g of adsorbent (BSA)  
Recommended packing conditions At a constant pressure of 1.5 bar (~ 22 psi)  
Recommended packing solutions ¡¡ 0.1 M NaCl solution or equilibration buffer  
Recommended operational flow rates Up to 600 cm/h  
Operating pH pH4.0– pH14.0  pH2.0– pH14.0 
Chemical stability All commonly used aqueous buffers and co-solvents  
Cleaning/sanitization 0.5 to 1.0 M NaOH  
Sterilization Autoclavable in 0.1M NaCl solution at 121°C for 30 minutes
Storage conditions 2 – 30°C , 20% ethanol, 0.2M sodium acetate 2 – 30°C , 20% ethanol