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 제품안내  세포배양   Stem Cell

hiPSC-Derived Sensory Neuron Progenitors

Sensory Neuron (감각뉴런)은 감각 기관에서 받아들인 정보를 중추 신경계인 뇌와 척수로 전달하는 뉴런입니다. Pain research에 이용되는 Human iPSC-derived sensory neurons Progenitor cell을 소개합니다.

AXOL
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특징
  • iPSC 유래 human Sensory Neuron Progenitors (from healthy male donor CD34+ cell)입니다.
  • 통각(nociception) 및 감각 습득에 관련된 다양한 channel을 발현합니다.
    1) Voltage gated sodium ion channel (Nav1.7)
    2) TTX-resistant channel (Nav1.8 / Nav 1.9)
    3) Temperature sensitive channel (TRPV1, TRPM8)
    4) 얼얼함, 쓰라림, 추위 sensor (TRPA1)
  • 배양하여 연구하고자 하는 development stage를 결정할 수 있습니다.
  • 통증 완화를 위한 약물 표적 및 말초 신경계 및 만성 통증의 장애를 조사하는데도 적합합니다.
  • Progenitor kit는 전용 media및 GDNF, BDNF, NGF, NT-3 등 성장과 분화에 필요한 grwoth factor를 함께 제공합니다.






  • Human iPSC-Derived Sensory Neuron Progenitor Kit (Male) (AX0057)의 구성
Cat. No. Product Name Format
ax0055 Human iPSC-Derived Sensory Neuron Progenitors (Male) 0.5M cells/vial
ax0060 Sensory Neuron Maintenance Medium 250ml
ax0033 Neural Plating-XF medium 30ml
ax0053 SureBond-XF 1ml
ax0052 SureBond+ReadySet SuraBond 3 x 120ul
ReadySet 2 x 10ml
ax139800 BDNF  10ug
ax139855 GDNF  10ug
ax139789 NGF  20ug
ax139811 NT-3  10ug



 iPSC-derived sensory neuron progenitors
 Cat. No. Product Name Donor
 ax0055 Human iPSC-Derived Sensory Neuron Progenitors
(Male)
Cord Blood CD34+ Cells
 ax0057 Human iPSC-Derived Sensory Neuron Progenitor Kit (Male) Cord Blood CD34+ Cells

 

 Sensory neuron culture media and reagents
 Cat. No.   Product Name Quantity
 ax0060   Sensory Neuron Maintenance Medium 250 ml
 ax0041+/ax0052   SureBond+ReadySet 3x120 μL vials & 2x10 mL vials
 ax0041XF/ax0053   SureBond-XF 1 mL
 ax139800   BDNF  10 μg / 100 μg
 ax139855   GDNF  10 μg / 100 μg
 ax139789   NGF  20 μg / 100 μg / 500 μg / 1 mg
 ax139811   NT-3  10 μg / 100 μg / 500 μg / 1 mg



 Phase Contrast

     Phase contrast images show the maturation of Axol Human iPSC-Derived Sensory Neuron Progenitors
     over two weeks after thawing and treatment with mitomycin C.



 Sodium ion channel expression

    Sodium channel RNA expression analysis by cDNA PCR 
     Axol iPSC-Derived Sensory Neuron Progenitors show RNA expression of all three voltage-gated
     sodium ion channels, Nav1.7, Nav1.8 and Nav1.9.


    Nav1.7 immunocytochemistry
      Axol Human iPSC-Derived Sensory Neuron Progenitors express Nav1.7, a key sodium ion channel in
      DRG sensory neurons that plays an important role in peripheral pain sensation.




    Nav1.7 and Nav1.8 immunocytochemistry  
      Mature iPSC-derived sensory neurons express the sodium ion channels, Nav1.7 and Nav1.8,
      and the maturation marker, doublecortin (DCX) after 6 weeks in culture.

   Sodium channel–mediated response to potassium chloride 
     Axol iPSC-Derived Sensory Neuron Progenitors have been shown to elicit a calcium response to
     the treatment of KCl at the soma signifying the presence of sodium channels.




   TTX-resistant Nav1.8 and Nav1.9
     Nociceptive sensory neurons are unique in that they contain voltage-gated inward current sodium
     channels (Nav 1.8 and Nav 1.9) that are resistant to tetrodotoxin (TTX). The presence of these
     TTX-resistant ion channels was confirmed in Axol iPSC-derived sensory neurons.



 Transient receptor potential ion channel expression

   TRPA1, TRPV1, Nav1.7  immunocytochemistry
     After 5 weeks in culture, Axol hiPSC-Derived Sensory Neurons express TRPV1, TRPA1 and Nav 1.7.       

   Neuronal firing responses to chemical stimuli:
     Wasabi (AITC), Capsaicin, Menthol



 Electrical activity

   Axol Human iPSC-Derived Sensory Neuron Progenitors display electrical activity.




  Protocol

주문정보(Ordering Information)
CATNUM PRODUCT SIZE
ax139800BDNF10 μg
ax139855GDNF10ug
ax0057Human iPSC-Derived Sensory Neuron Progenitor Kit (Male)1 kit
ax0055Human iPSC-Derived Sensory Neuron Progenitors (Male)1 vial
ax0055Human iPSC-Derived Sensory Neuron Progenitors (Male) (500,000 cells & Neural Plating-XF Medium)1 Kit
ax139789NGF20ug
ax139811NT-310ug
ax0060Sensory Neuron Maintenance Medium 250ml
ax0041+/ax0052SureBond+ReadySet3x120 μL vials & 2x10 mL vials
ax0041XF/ax0053SureBond-XF1 mL