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 제품안내  면역학   Stem Cell

Human iPSC-derived Sensory Neuron Progenitor Cells

Sensory Neuron (감각뉴런)은 감각 기관에서 받아들인 정보를 중추 신경계인 뇌와 척수로 전달하는 뉴런입니다. Pain research에 이용되는 Human iPSC-derived sensory neurons Progenitor cell을 소개합니다.

Axol
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특징
  • iPSC 유래 human Sensory Neuron Progenitors (from healthy male donor CD34+ cell)입니다.
  • 인간의 통각(nociception)에 필수적인 voltage gated ion channels (Nav1.7 / Nav1.8 / Nav 1.9)과 transient receptor potential (TRP) ion channels (TRPV1, TRPM8, TRPA1)를 발현합니다.
  • 배양하여 연구하고자 하는 development stage를 결정할 수 있습니다.
  • 통증 완화를 위한 약물 표적 및 말초 신경계 및 만성 통증의 장애를 조사하는데도 적합합니다.
  • Glass 및 Plastic에서의 성장과 생존을 위한 전용 media를 함께 공급합니다.
    • ax0041+/ax0052 (SureBond+ReadySet) : Coating reagent needed for endpoint assays on glass 
    • ax0041XF/ax0053 (SureBond-XF) : Xeno-free coating reagent needed for endpoint assays on plastic



 Phase Contrast

     Phase contrast images show the maturation of Axol Human iPSC-Derived Sensory Neuron Progenitors
     over two weeks after thawing and treatment with mitomycin C.



 Sodium ion channel expression

    Sodium channel RNA expression analysis by cDNA PCR 
     Axol iPSC-Derived Sensory Neuron Progenitors show RNA expression of all three voltage-gated
     sodium ion channels, Nav1.7, Nav1.8 and Nav1.9.


    Nav1.7 immunocytochemistry
      Axol Human iPSC-Derived Sensory Neuron Progenitors express Nav1.7, a key sodium ion channel in
      DRG sensory neurons that plays an important role in peripheral pain sensation.




    Nav1.7 and Nav1.8 immunocytochemistry  
      Mature iPSC-derived sensory neurons express the sodium ion channels, Nav1.7 and Nav1.8,
      and the maturation marker, doublecortin (DCX) after 6 weeks in culture.

   Sodium channel–mediated response to potassium chloride 
     Axol iPSC-Derived Sensory Neuron Progenitors have been shown to elicit a calcium response to
     the treatment of KCl at the soma signifying the presence of sodium channels.




   TTX-resistant Nav1.8 and Nav1.9
     Nociceptive sensory neurons are unique in that they contain voltage-gated inward current sodium
     channels (Nav 1.8 and Nav 1.9) that are resistant to tetrodotoxin (TTX). The presence of these
     TTX-resistant ion channels was confirmed in Axol iPSC-derived sensory neurons.



 Transient receptor potential ion channel expression

   TRPA1, TRPV1, Nav1.7  immunocytochemistry
     After 5 weeks in culture, Axol hiPSC-Derived Sensory Neurons express TRPV1, TRPA1 and Nav 1.7.       

   Neuronal firing responses to chemical stimuli:
     Wasabi (AITC), Capsaicin, Menthol



 Electrical activity

   Axol Human iPSC-Derived Sensory Neuron Progenitors display electrical activity.






 

주문정보(Ordering Information)
CATNUM PRODUCT SIZE
ax0055Human iPSC-Derived Sensory Neuron Progenitors (Male) (500,000 cells & Neural Plating-XF Medium)1 Kit
ax0060Sensory Neuron Maintenance Medium 250ml
ax0041+/ax0052SureBond+ReadySet (3 x 120 μL vials & 2 x 10 mL vials)1 Kit
ax0041XF/ax0053SureBond-XF 1ml