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세포 및 조직에서 Cas9를 측정 및 정량하는 ELISA Kit 입니다.
| Example of Results :
The following figures demonstrate typical results
with the Cas9 ELISA Kit. One should use the
data below for reference only. This data should
not be used to interpret actual results.
| Detection of Cas9 in Transfected 293 cells :
Cells were transiently transfected with a Cas9
mammalian expression vector or mock transfected.
After 48 hours, cells were lysed in RIPA buffer and
protein concentration was determined. The Cas9
ELISA kit was performed in the absence of cell
lysate (Neg), the presence of 50 ng/mL Cas9
Nuclease (Pos) (NEB catalog number M0386),
the presence of 10 μg protein lysate of mock
transfected (Mock), or Cas9 transfected (Cas9).