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 ǰȳ  ü,׿,ŶƮ   Immunoassays

HMGB1 Antibodies/Protein/ELISA Kit

HMGB1 (High Mobility Group Box 1) ٳ gene transcription, gene regulation, DNA repair ϴ DNA-Binding protein ϰ, cytosol Beclin-1 ȣۿϿ autophagy մϴ.

HMGB1 Ǹ chemokine̳ DAMP(Damage-associated molecular pattern)μ Ͽ 鿪 Ȱȭ մϴ.

HMGB1 õ ü, ܹ, ELISA kit Ұմϴ.

Arigo
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  • Human HMGB1 ELISA Kit (ARG81185)
Product Description ARG81185 Human HMGB1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human HMGB1 in plasma and cell culture supernatant.
Tested Reactivity Hu
Tested Application ELISA
Specificity This Human HMGB1 ELISA kit detect natural and recombinant Human HMGB1 protein and the cross-reactivity with HMGB2 is lower than <2%.
Target Name HMGB1
Sensitivity 0.3 ng/ml
Sample Type Plasma, and cell culture supernatant
Standard Range 0.3125 - 20 ng/ml
Sample Volume 100 µl
Precision < 5.2%
Full Name high mobility group box 1
Alternate Names HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3



 

  • Mouse/Rat HMGB1 ELISA Kit (ARG81310)
Product Description ARG81310 Mouse/Rat HMGB1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse/Rat HMGB1 in plasma, cell culture supernatants and cell/tissue lysate samples.
Tested Reactivity Ms, Rat
Tested Application ELISA
Specificity Cross-react to mouse HMGB2 is less than 5%.
Target Name HMGB1
Conjugation HRP (Substrate: TMB and read at 450 nm)
Sensitivity 0.3 ng/ml
Sample Type Plasma, and cell culture supernatant
Standard Range 0.3125 - 20 ng/ml
Sample Volume 100 µl
Precision < 5.2%
Full Name high mobility group box 1
Alternate Names HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3

 

  • HMGB1 ELISA Kit (ARG81351)
Product Description ARG81351 HMGB1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human/Mouse/Rat HMGB1 in serum samples.
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA
Specificity No significant cross-reactivity or interference with recombinant human HMGB2 was observed. 
Target Name HMGB1
Conjugation HRP (Substrate: TMB and read at 450 nm)
Sensitivity 0.4 ng/ml
Sample Type Serum 
Standard Range 0.625 - 20 ng/ml 
Sample Volume 50 µl
Full Name high mobility group box 1
Alternate Names HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3

 

  • anti-HMGB1 antibody (ARG55034) 
Product Description Rabbit Polyclonal antibody recognizes HMGB1
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name HMGB1
Antigen Species Human
Immunogen Recombinant protein of Human HMGB1 (NP_002119.1)
Conjugation Un-conjugated
Full Name high mobility group box 1
Alternate Names HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3



  • anti-HMGB1 antibody (ARG65636)
Product Description Rabbit Polyclonal antibody recognizes HMGB1
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name HMGB1
Antigen Species Human
Immunogen Synthetic peptide of human HMGB1
Conjugation Un-conjugated
Full Name high mobility group box 1
Alternate Names HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3

 

  • anti-HMGB1 antibody [1F3] (ARG10756)
Product Description Mouse Monoclonal to Anti-High-mobility group protein B1, HMGB1
Tested Reactivity Hu
Tested Application WB, ICC/IF, IHC
Host Mouse
Clonality Monoclonal
Clone 1F3
Isotype IgG2b
Target Name HMGB1
Antigen Species Human
Immunogen Human full length protein expressed and purified form E. coli.
Conjugation Un-conjugated
Full Name high mobility group box 1
Alternate Names HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3

 

  • anti-HMGB1 antibody [SQab1711] (ARG65863)
Product Description Mouse Monoclonal antibody [SQab1711] recognizes HMGB1
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, FACS, ICC/IF, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone SQab1711
Isotype IgG2b
Target Name HMGB1
Immunogen OVA-conjugated synthetic peptide of HMGB1.
Conjugation Un-conjugated
Full Name high mobility group box 1
Alternate Names HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3



  • anti-HMGB1 Neutralizing antibody [SQab20175] (ARG66714)
Product Description Low endotoxin Mouse Monoclonal antibody [SQab20175] recognizes HMGB1
Tested Reactivity Hu, Ms, Bov 
Tested Application ELISA, FACS, Neut, WB
Host Mouse
Clonality Monoclonal
Clone SQab20175
Isotype IgM 
Target Name HMGB1 
Antigen Species Human
Immunogen Human HMGB1. 
Conjugation Un-conjugated 
Full Name high mobility group box 1
Alternate Names HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3






  • Human HMGB1 recombinant protein (Active) (His-tagged, C-ter) (ARG70220)
Product Description E. coli expressed, His-tagged (C-ter) Active Human HMGB1 recombinant protein.
Tested Reactivity Hu, Ms
Tested Application FuncSt, SDS-PAGE
Species Human
AA Sequence MGKGD PKKPR GKMSS YAFFV QTCRE EHKKK HPDAS VNFSE FSKKC SERWK TMSAK EKGKF EDMAK ADKAR YEREM KTYIP PKGET KKKFK DPNAP KRPPS AFFLF CSEYR PKIKG EHPGL SIGDV AKKLG EMWNN TAADD KQPYE KKAAK LKEKY EKDIA AYRAK GKPDA AKKGV VKAEK SKKKK EEEED EEDEE DEEEE EDEED EDEEE DDDDE with polyhistidine tag at the C-terminus.
Activity Active
Activity Note Measure by its ability to induce TNF alpha in Raw264.7 cells.
The ED50 for this effect is < 10 μg/ml. This protein also induces TNF alpha releasing from human A549 cultured cells based on customer feedback.
Purification Ni-NTA chromatography
Purification Note Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 98% (by SDS-PAGE)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.



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