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 제품안내  맞춤 서비스   항체제작 서비스

Rabbit monoclonal antibody 제작서비스

높은 binding affinity의 Rabbit Monoclonal 항체를 만들어드립니다.

Prosci
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특징
  • higher binding affinity monoclonal antibody
  • immunization후에 plasma cell을 isolation해서 100% plasma cells사용
  • plasma cell로 인한 신속한 screening time


    [기존 monoclonal technology와 비교]    

   Hybridoma  Phase display  Single B Cell Cloning  Single Plasma Cell Cloning
 Start Material  B Cells  B Cells  B Cells  Plasma Cells
 Efficiency  ≤ 1% Plasma Cells  ≤ 1% Plasma Cells  ≤ 1% Plasma Cells  100% Plasma Cells
 Screening time  12 weeks  6 weeks  3 weeks  3 weeks
 mAb Diversity  Medium  Low  High  High



              

         Procedure  Detailed Procedure
 Phase I      Immunization ㆍImmunization of 3 rabbits on a 10-week or 15-week protocol using either
   CFA/IFA or Adjuplex TM
ㆍELISA on each bleed screened against antigen
ㆍFinal boost and bone marrow isolation for 1 rabbit
 Phase II  Plasma Cell isolation ㆍPlasma cell isolation with one cell per well - 2 days
 Phase III   Gene Amplification
            &
        Cloning
ㆍAmplification of heavy and light chains from one 96-well plate of plasma cells
ㆍCloning of the heavy and light chains into expression vectors - 7 days
 Phase IV       Expression
            &
       Screening
ㆍExpression of cloned heavy and light chain genes into HEK293 cells
ㆍScreening of supernatants by indirect ELISA against the antigen
ㆍPlasmid DNA & sequence for 1 final clone
ㆍ200 ug of up to 5 clones - 11 days
 Phase V  Antibody production ㆍExpression and Protein A purification of rabbit monoclonal antibodies from 
   1mg to > 300 mg - 14~28 days