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 제품안내  형광시약   Protein Labeling

TrueBlack Lipofuscin

TrueBlack™ 은 Tissue section의 immunofluorescence staining시 lipofuscin autofluorescence를 제거하는 시약입니다.

Biotium
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   특징

  • 노화된 세포에서 축적되어 자체 발광을 보이는 Lipofuscin의 형광성을 제거하는 시약입니다.
  • Non-lipofusin source의 autofluorescence도 낮출 수 있습니다.
  • Sudan Black B와는 달리 high background 염려가 없습니다.
  • Human과 aged animal tissues의 fluorescence imaging을 원활하게 할 수 있습니다.
  • 형광 항체 및 핵 대조염색제와도 함께 사용할 수 있습니다.

적용
         
         Sudan Black B에 비해 백그라운드가 적습니다.

 Lipofuscin autofluorescence in methanol-fixed adult human tissue sections. In untreated tissue (top row), lipofuscin appeared as fluorescent granules that fluoresced in all fluorescence channels. Sudan Black B (middle row) masked lipofuscin autofluorescence, but introduced background in the red and far-red channels. TrueBlack (bottom row) masked lipofuscin with minimal increase in fluorescence background (bottom row). Methanol-fixed cryosections of adult human cerebral cortex were left untreated or stained with 0.1% Sudan Black B in 70% ethanol or 1X TrueBlack according to product protocol. Samples were imaged at the same gain settings on a Zeiss LSM 700 confocal microscope in the FITC (green), Cy3 (red), and Cy5 (far-red) channels.

 

       TrueBlack  사용전과 사용후 비교


 Quenching of lipofuscin autofluorescence using TrueBlack post-treatment after immunofluorescence staining. Methanol-fixed cryosections of human cerebral cortex were stained with rabbit anti-GFAP followed by CF640R goat anti-rabbit (cat. no. 20176) and DAPI (cat. no. 40009). Sections were left untreated or treated with TrueBlack, then mounted in EverBrite mounting medium (cat. no. 23001). Untreated sections (left) showed lipofuscin autofluorescence (white arrows) in addition to GFAP immunofluorescence (glial processes, magenta) and DAPI staining (nuclei, blue). TrueBlack (right) eliminated lipofuscin fluorescence while maintaining good signal-to-noise for GFAP immunostaining in the far-red channel (magenta) and nuclear counterstaining (blue).

 다른 소스로부터 발생하는 autofluorescence도 제거해 줍니다.

TrueBlack reduces non-lipofuscin autofluorescence in human kidney sections in the FITC, Cy3, and Cy5 channels. Human kidney cryosections were fixed in ice-cold methanol and nuclei were stained with DAPI (blue). Autofluorescence was imaged on a Zeiss LSM700 confocal microscope in all channels with the same imaging settings for both samples.Top panel: untreated; Bottom panel: TrueBlack-treated.