- 견적 및 주문
- 제품/실험 Q&A
TrueBlack™ 은 Tissue section의 immunofluorescence staining시 lipofuscin autofluorescence를 제거하는 시약입니다.
|Lipofuscin autofluorescence in methanol-fixed adult human tissue sections. In untreated tissue (top row), lipofuscin appeared as fluorescent granules that fluoresced in all fluorescence channels. Sudan Black B (middle row) masked lipofuscin autofluorescence, but introduced background in the red and far-red channels. TrueBlack (bottom row) masked lipofuscin with minimal increase in fluorescence background (bottom row). Methanol-fixed cryosections of adult human cerebral cortex were left untreated or stained with 0.1% Sudan Black B in 70% ethanol or 1X TrueBlack according to product protocol. Samples were imaged at the same gain settings on a Zeiss LSM 700 confocal microscope in the FITC (green), Cy3 (red), and Cy5 (far-red) channels.|
TrueBlack 사용전과 사용후 비교
|Quenching of lipofuscin autofluorescence using TrueBlack post-treatment after immunofluorescence staining. Methanol-fixed cryosections of human cerebral cortex were stained with rabbit anti-GFAP followed by CF640R goat anti-rabbit (cat. no. 20176) and DAPI (cat. no. 40009). Sections were left untreated or treated with TrueBlack, then mounted in EverBrite mounting medium (cat. no. 23001). Untreated sections (left) showed lipofuscin autofluorescence (white arrows) in addition to GFAP immunofluorescence (glial processes, magenta) and DAPI staining (nuclei, blue). TrueBlack (right) eliminated lipofuscin fluorescence while maintaining good signal-to-noise for GFAP immunostaining in the far-red channel (magenta) and nuclear counterstaining (blue).|
다른 소스로부터 발생하는 autofluorescence도 제거해 줍니다.
|TrueBlack reduces non-lipofuscin autofluorescence in human kidney sections in the FITC, Cy3, and Cy5 channels. Human kidney cryosections were fixed in ice-cold methanol and nuclei were stained with DAPI (blue). Autofluorescence was imaged on a Zeiss LSM700 confocal microscope in all channels with the same imaging settings for both samples.Top panel: untreated; Bottom panel: TrueBlack-treated.|
COPYRIGHT(c) 2008. 고마바이오텍(주). ALL RIGHTS RESERVED. Contact Webmaster for more information.
개인정보취급방침 (07207) 서울시 영등포구 양평로 21길 26 선유도역1차 아이에스비즈타워 19층 | TEL 02)579-8787 | FAX 02)578-7042.