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 제품안내  세포배양   Stem Cell

hiPSC-Derived Cortical Neural Stem Cells

다양한 donor의 cell에 iPSC technology를 도입한 cerebral cortical neural stem cells (NSCs) 입니다.

AXOL
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특징
  • Fully defined neural induction 조건 하에 iPSC technology를 통해 제작된 cerebral cortex 유래 neural stem cell 입니다.
  • cerebral cortical neural stem cell과 progenitor cell에서 특징적으로 보이는 PAX6, FOXG1 marker를 확인할 수 있습니다.
  • NSCs로부터 electrically active 한 cortical neuron과 in vitro functional synapses, neural circuits을 generation 할 수 있습니다.
  • 모든 cell은 1.5M cells/vial로 제공되며 구매 시 30 mL의 plating medium을 함께 드립니다. 
  • P0 state로 제공 되므로 여러 번의 expansion을 거쳐 많은 수의 cell을 확보할 수 있습니다.
  • AxolGEM은 CRISPR/Cas9 gene editing을 통해 Parkinson's disease와 Alzheimer's disease 발병의 주요 원인으로 알려진 LRRK2와 MAPT gene을 edition한 NSCs 입니다. 
  • 배양에 최적화된 medium을 함께 공급합니다.

 
 

 Human iPSC-derived neural stem cells from healthy donors
Cat. No. Product Name Donor
ax0011 Human iPSC-Derived Neural Stem Cells (Male) Cord Blood CD34+ Cells
ax0015 Human iPSC-Derived Neural Stem Cells (Male) Cord Blood CD34+ Cells
ax0016 Human iPSC-Derived Neural Stem Cells (Female) Cord Blood CD34+ Cells
ax0018 Human iPSC-Derived Neural Stem Cells (Male) Fibroblasts (74 Yr Donor)
ax0019 Human iPSC-Derived Neural Stem Cells (Female) Fibroblasts (64 Yr Donor)

 

 Human iPSC-derived neural stem cells from patient donors 
Cat. No. Product Name Donor
ax0111 Human iPSC-Derived Neural Stem Cells -
Alzheimer's Disease Patient (APOE4 HOM)
Fibroblasts (87 Yr Female)
ax0112 Human iPSC-Derived Neural Stem Cells -
Alzheimer's Disease Patient (PSEN1 L286V)
Fibroblasts (38 Yr Female)
ax0113 Human iPSC-Derived Neural Stem Cells -
Alzheimer's Disease Patient (PSEN1 M146L)
Fibroblasts (53 Yr Male)
ax0114 Human iPSC-Derived Neural Stem Cells -
Alzheimer's Disease Patient (PSEN1 A246E)
Fibroblasts (31 Yr Female)
ax0211 Human iPSC-Derived Neural Stem Cells -
Huntington's Disease Patient
Fibroblasts (48 Yr Female)
ax0411 Human iPSC-Derived Neural Stem Cells -
Epilepsy Patient 
Fibroblasts (5 Mo Female)
ax1001 Human iPSC-Derived Neural Stem Cells -
Trisomy X Patient 
Fibroblasts (74 Yr Female)

 

 axolGEM (genetically edited model) human iPSC-derived neural stem cells 
Cat. No. Product Name Donor
ax0019 Human iPSC-Derived Neural Stem Cells (Female)
Isogenic Control
Fibroblasts (64 Yr Donor)
ax0310 axolGEM iPSC-Derived Neural Stem Cells
LRRK2 G2019S HOM 
Fibroblasts (64 Yr Donor)
ax0311 axolGEM iPSC-Derived Neural Stem Cells
LRRK2 G2019S HET 
Fibroblasts (64 Yr Donor)
ax0320 axolGEM iPSC-Derived Neural Stem Cells
MAPT R406W HOM 
Fibroblasts (64 Yr Donor)
ax0321 axolGEM iPSC-Derived Neural Stem Cells
MAPT R406W HET 
Fibroblasts (64 Yr Donor)
ax0322 axolGEM iPSC-Derived Neural Stem Cells
MAPT V337M HOM 
Fibroblasts (64 Yr Donor)
ax0323 axolGEM iPSC-Derived Neural Stem Cells
MAPT V337M HET 
Fibroblasts (64 Yr Donor)
ax0324 axolGEM iPSC-Derived Neural Stem Cells
MAPT P301L HOM 
Fibroblasts (64 Yr Donor)
ax0325 axolGEM iPSC-Derived Neural Stem Cells
MAPT P301L HET 
Fibroblasts (64 Yr Donor)



 Neural cell culture media and reagents 
Cat. No. Product Name Quantity
ax0101 Neural Stem Cell Media & Reagent Bundle -
Expansion and Synchronous Differentiation (System A)
Kit
ax0102 Neural Stem Cell Media & Reagent Bundle -
Synchronous Differentiation (System B)
Kit
ax0103 Neural Stem Cell Media & Reagent Bundle -
Expansion and Spontaneous Differentiation (System C)
Kit
ax0104 Neural Stem Cell Media & Reagent Bundle -
Spontaneous Differentiation (System D)
Kit
ax0030-500 Neural Expansion-XF Medium 500 mL
ax0034-125 Neural Differentiation-XF Medium 125 mL
ax0032-500 Neural Maintenance-XF Medium 500 mL
ax0033 Neural Plating-XF Medium 30 mL
ax0041 SureBond 3 x 120 μL 
ax0052 SureBond+ReadySet 3 x 120 μL & 2 x 10 mL
ax0053 SureBond-XF 1 mL
ax0044 Unlock 25 mL
ax0054 Unlock-XF 100 mL
ax0047 Recombinant Human FGF2 100 μg
ax0048 Recombinant Human EGF 100 μg
ax0051 ReadyFect 40 μL




  • Transfection
  • Neurite outgrowth
  • Patch clamp
  • Multi-electrode array (MEA)
  • Transplatation




  • Phase contrast

     Axol iPSC derived Neural Stem Cells grown in Neural Expansion XF Medium with the addition of EGF and EGF2. 



  • Immunofluorescence


   







  • Transfection


        ReadyFect leads to 30-40% transfection efficiency. The image demonstrates transfection results using 3 µL of
        ReadyFect / 1 µg of a GFP-encoding vector (pVectOZ-GFP). GFP expression was evaluated two days
        post-transfection.

  • Neurite Outgrowth



  • Patch Clamp


  • MEA Analysis





   Protocol


  Product citations

주문정보(Ordering Information)
CATNUM PRODUCT SIZE
ax0311axolGEM iPSC-Derived Neural Stem Cells LRRK2 G2019S HET1 vial
ax0310axolGEM iPSC-Derived Neural Stem Cells LRRK2 G2019S HOM1 vial
ax0325axolGEM iPSC-Derived Neural Stem Cells MAPT P301L HET1 vial
ax0324axolGEM iPSC-Derived Neural Stem Cells MAPT P301L HOM1 vial
ax0321axolGEM iPSC-Derived Neural Stem Cells MAPT R406W HET1 vial
ax0320axolGEM iPSC-Derived Neural Stem Cells MAPT R406W HOM1 vial
ax0323axolGEM iPSC-Derived Neural Stem Cells MAPT V337M HET1 vial
ax0322axolGEM iPSC-Derived Neural Stem Cells MAPT V337M HOM1 vial
ax0016Human iPSC-Derived Neural Stem Cells (Female)1 vial
ax0019Human iPSC-Derived Neural Stem Cells (Female)1 vial
ax0011Human iPSC-Derived Neural Stem Cells (Male)1 vial
ax0015Human iPSC-Derived Neural Stem Cells (Male)1 vial
ax0018Human iPSC-Derived Neural Stem Cells (Male)1 vial
ax0111Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (APOE4 HOM)1 vial
ax0114Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (PSEN1 A246E)1 vial
ax0112Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (PSEN1 L286V)1 vial
ax0113Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (PSEN1 M146L)1 vial
ax0411Human iPSC-Derived Neural Stem Cells - Epilepsy Patient1 vial
ax0211Human iPSC-Derived Neural Stem Cells - Huntington's Disease Patient1 vial
ax1001Human iPSC-Derived Neural Stem Cells - Trisomy X Patient1 vial
ax0034-125Neural Differentiation-XF Medium125 mL
ax0030-500Neural Expansion-XF Medium500 mL
ax0032-500Neural Maintenance-XF Medium500 mL
ax0033Neural Plating-XF Medium30 mL
ax0103Neural Stem Cell Media & Reagent Bundle - Expansion and Spontaneous Differentiation (System C)Kit
ax0101Neural Stem Cell Media & Reagent Bundle - Expansion and Synchronous Differentiation (System A)Kit
ax0104Neural Stem Cell Media & Reagent Bundle - Spontaneous Differentiation (System D)Kit
ax0102Neural Stem Cell Media & Reagent Bundle - Synchronous Differentiation (System B)Kit
ax0051ReadyFect40 μL
ax0048Recombinant Human EGF100 μg
ax0047Recombinant Human FGF2100 μg
ax0041SureBond3 x 120 μL
ax0052SureBond+ReadySet3 x 120 μL & 2 x 10 mL
ax0053SureBond-XF1 mL
ax0044Unlock25 mL
ax0054Unlock-XF100 mL