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 Á¦Ç°¾È³»  ¼¼Æ÷¹è¾ç   Stem Cell

hiPSC-Derived Cortical Neural Stem Cells

´Ù¾çÇÑ donorÀÇ cell¿¡ iPSC technology¸¦ µµÀÔÇÑ cerebral cortical neural stem cells (NSCs) ÀÔ´Ï´Ù.

AXOL
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  • Fully defined neural induction Á¶°Ç ÇÏ¿¡ iPSC technology¸¦ ÅëÇØ Á¦ÀÛµÈ cerebral cortex À¯·¡ neural stem cell ÀÔ´Ï´Ù.
  • cerebral cortical neural stem cell°ú progenitor cell¿¡¼­ Ư¡ÀûÀ¸·Î º¸ÀÌ´Â PAX6, FOXG1 marker¸¦ È®ÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù.
  • NSCs·ÎºÎÅÍ electrically active ÇÑ cortical neuron°ú in vitro functional synapses, neural circuitsÀ» generation ÇÒ ¼ö ÀÖ½À´Ï´Ù.
  • ¸ðµç cellÀº 1.5M cells/vial·Î Á¦°øµÇ¸ç ±¸¸Å ½Ã 30 mLÀÇ plating mediumÀ» ÇÔ²² µå¸³´Ï´Ù. 
  • P0 state·Î Á¦°ø µÇ¹Ç·Î ¿©·¯ ¹øÀÇ expansionÀ» °ÅÃÄ ¸¹Àº ¼öÀÇ cellÀ» È®º¸ÇÒ ¼ö ÀÖ½À´Ï´Ù.
  • AxolGEMÀº CRISPR/Cas9 gene editingÀ» ÅëÇØ Parkinson's disease¿Í Alzheimer's disease ¹ßº´ÀÇ ÁÖ¿ä ¿øÀÎÀ¸·Î ¾Ë·ÁÁø LRRK2¿Í MAPT geneÀ» editionÇÑ NSCs ÀÔ´Ï´Ù. 
  • ¹è¾ç¿¡ ÃÖÀûÈ­µÈ mediumÀ» ÇÔ²² °ø±ÞÇÕ´Ï´Ù.

 
 

 Human iPSC-derived neural stem cells from healthy donors
Cat. No. Product Name Donor
ax0011 Human iPSC-Derived Neural Stem Cells (Male) Cord Blood CD34+ Cells
ax0015 Human iPSC-Derived Neural Stem Cells (Male) Cord Blood CD34+ Cells
ax0016 Human iPSC-Derived Neural Stem Cells (Female) Cord Blood CD34+ Cells
ax0018 Human iPSC-Derived Neural Stem Cells (Male) Fibroblasts (74 Yr Donor)
ax0019 Human iPSC-Derived Neural Stem Cells (Female) Fibroblasts (64 Yr Donor)

 

 Human iPSC-derived neural stem cells from patient donors 
Cat. No. Product Name Donor
ax0111 Human iPSC-Derived Neural Stem Cells -
Alzheimer's Disease Patient (APOE4 HOM)
Fibroblasts (87 Yr Female)
ax0112 Human iPSC-Derived Neural Stem Cells -
Alzheimer's Disease Patient (PSEN1 L286V)
Fibroblasts (38 Yr Female)
ax0113 Human iPSC-Derived Neural Stem Cells -
Alzheimer's Disease Patient (PSEN1 M146L)
Fibroblasts (53 Yr Male)
ax0114 Human iPSC-Derived Neural Stem Cells -
Alzheimer's Disease Patient (PSEN1 A246E)
Fibroblasts (31 Yr Female)
ax0211 Human iPSC-Derived Neural Stem Cells -
Huntington's Disease Patient
Fibroblasts (48 Yr Female)
ax0411 Human iPSC-Derived Neural Stem Cells -
Epilepsy Patient 
Fibroblasts (5 Mo Female)
ax1001 Human iPSC-Derived Neural Stem Cells -
Trisomy X Patient 
Fibroblasts (74 Yr Female)

 

 axolGEM (genetically edited model) human iPSC-derived neural stem cells 
Cat. No. Product Name Donor
ax0019 Human iPSC-Derived Neural Stem Cells (Female)
Isogenic Control
Fibroblasts (64 Yr Donor)
ax0310 axolGEM iPSC-Derived Neural Stem Cells
LRRK2 G2019S HOM 
Fibroblasts (64 Yr Donor)
ax0311 axolGEM iPSC-Derived Neural Stem Cells
LRRK2 G2019S HET 
Fibroblasts (64 Yr Donor)
ax0320 axolGEM iPSC-Derived Neural Stem Cells
MAPT R406W HOM 
Fibroblasts (64 Yr Donor)
ax0321 axolGEM iPSC-Derived Neural Stem Cells
MAPT R406W HET 
Fibroblasts (64 Yr Donor)
ax0322 axolGEM iPSC-Derived Neural Stem Cells
MAPT V337M HOM 
Fibroblasts (64 Yr Donor)
ax0323 axolGEM iPSC-Derived Neural Stem Cells
MAPT V337M HET 
Fibroblasts (64 Yr Donor)
ax0324 axolGEM iPSC-Derived Neural Stem Cells
MAPT P301L HOM 
Fibroblasts (64 Yr Donor)
ax0325 axolGEM iPSC-Derived Neural Stem Cells
MAPT P301L HET 
Fibroblasts (64 Yr Donor)



 Neural cell culture media and reagents 
Cat. No. Product Name Quantity
ax0101 Neural Stem Cell Media & Reagent Bundle -
Expansion and Synchronous Differentiation (System A)
Kit
ax0102 Neural Stem Cell Media & Reagent Bundle -
Synchronous Differentiation (System B)
Kit
ax0103 Neural Stem Cell Media & Reagent Bundle -
Expansion and Spontaneous Differentiation (System C)
Kit
ax0104 Neural Stem Cell Media & Reagent Bundle -
Spontaneous Differentiation (System D)
Kit
ax0030-500 Neural Expansion-XF Medium 500 mL
ax0034-125 Neural Differentiation-XF Medium 125 mL
ax0032-500 Neural Maintenance-XF Medium 500 mL
ax0033 Neural Plating-XF Medium 30 mL
ax0041 SureBond 3 x 120 μL 
ax0052 SureBond+ReadySet 3 x 120 μL & 2 x 10 mL
ax0053 SureBond-XF 1 mL
ax0044 Unlock 25 mL
ax0054 Unlock-XF 100 mL
ax0047 Recombinant Human FGF2 100 μg
ax0048 Recombinant Human EGF 100 μg
ax0051 ReadyFect 40 μL




  • Transfection
  • Neurite outgrowth
  • Patch clamp
  • Multi-electrode array (MEA)
  • Transplatation




  • Phase contrast

     Axol iPSC derived Neural Stem Cells grown in Neural Expansion XF Medium with the addition of EGF and EGF2. 



  • Immunofluorescence


   







  • Transfection


        ReadyFect leads to 30-40% transfection efficiency. The image demonstrates transfection results using 3 µL of
        ReadyFect / 1 µg of a GFP-encoding vector (pVectOZ-GFP). GFP expression was evaluated two days
        post-transfection.

  • Neurite Outgrowth



  • Patch Clamp


  • MEA Analysis





   Protocol


  Product citations

ÁÖ¹®Á¤º¸(Ordering Information)
CATNUM PRODUCT SIZE
ax0311axolGEM iPSC-Derived Neural Stem Cells LRRK2 G2019S HET1 vial
ax0310axolGEM iPSC-Derived Neural Stem Cells LRRK2 G2019S HOM1 vial
ax0325axolGEM iPSC-Derived Neural Stem Cells MAPT P301L HET1 vial
ax0324axolGEM iPSC-Derived Neural Stem Cells MAPT P301L HOM1 vial
ax0321axolGEM iPSC-Derived Neural Stem Cells MAPT R406W HET1 vial
ax0320axolGEM iPSC-Derived Neural Stem Cells MAPT R406W HOM1 vial
ax0323axolGEM iPSC-Derived Neural Stem Cells MAPT V337M HET1 vial
ax0322axolGEM iPSC-Derived Neural Stem Cells MAPT V337M HOM1 vial
ax0016Human iPSC-Derived Neural Stem Cells (Female)1 vial
ax0019Human iPSC-Derived Neural Stem Cells (Female)1 vial
ax0011Human iPSC-Derived Neural Stem Cells (Male)1 vial
ax0015Human iPSC-Derived Neural Stem Cells (Male)1 vial
ax0018Human iPSC-Derived Neural Stem Cells (Male)1 vial
ax0111Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (APOE4 HOM)1 vial
ax0114Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (PSEN1 A246E)1 vial
ax0112Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (PSEN1 L286V)1 vial
ax0113Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (PSEN1 M146L)1 vial
ax0411Human iPSC-Derived Neural Stem Cells - Epilepsy Patient1 vial
ax0211Human iPSC-Derived Neural Stem Cells - Huntington's Disease Patient1 vial
ax1001Human iPSC-Derived Neural Stem Cells - Trisomy X Patient1 vial
ax0034-125Neural Differentiation-XF Medium125 mL
ax0032-500Neural Maintenance-XF Medium500 mL
ax0033Neural Plating-XF Medium30 mL
ax0103Neural Stem Cell Media & Reagent Bundle - Expansion and Spontaneous Differentiation (System C)Kit
ax0101Neural Stem Cell Media & Reagent Bundle - Expansion and Synchronous Differentiation (System A)Kit
ax0104Neural Stem Cell Media & Reagent Bundle - Spontaneous Differentiation (System D)Kit
ax0102Neural Stem Cell Media & Reagent Bundle - Synchronous Differentiation (System B)Kit
ax0051ReadyFect40 ¥ìL
ax0048Recombinant Human EGF100 ¥ìg
ax0047Recombinant Human FGF2100 ¥ìg
ax0041SureBond3 x 120 ¥ìL
ax0052SureBond+ReadySet3 x 120 ¥ìL & 2 x 10 mL
ax0053SureBond-XF1 mL
ax0044Unlock25 mL
ax0054Unlock-XF100 mL