• Fully defined neural induction 조건 하에 iPSC technology를 통해 제작된 cerebral cortex 유래 neural stem cell 입니다.
• cerebral cortical neural stem cell과 progenitor cell에서 특징적으로 보이는 PAX6, FOXG1 marker를 확인할 수 있습니다.
• NSCs로부터 electrically active 한 cortical neuron과 in vitro functional synapses, neural circuits을 generation 할 수 있습니다.
• 모든 cell은 1.5M cells/vial로 제공되며 구매 시 30 mL의 plating medium을 함께 드립니다. 
• P0 state로 제공 되므로 여러 번의 expansion을 거쳐 많은 수의 cell을 확보할 수 있습니다.
• AxolGEM은 CRISPR/Cas9 gene editing을 통해 Parkinson's disease와 Alzheimer's disease 발병의 주요 원인으로 알려진 LRRK2와 MAPT gene을 edition한 NSCs 입니다. 
• 배양에 최적화된 medium을 함께 공급합니다.

 
 

Human iPSC-derived neural stem cells from healthy donors
Cat. No.	Product Name	Donor
ax0011	Human iPSC-Derived Neural Stem Cells (Male)	Cord Blood CD34+ Cells
ax0015	Human iPSC-Derived Neural Stem Cells (Male)	Cord Blood CD34+ Cells
ax0016	Human iPSC-Derived Neural Stem Cells (Female)	Cord Blood CD34+ Cells
ax0018	Human iPSC-Derived Neural Stem Cells (Male)	Fibroblasts (74 Yr Donor)
ax0019	Human iPSC-Derived Neural Stem Cells (Female)	Fibroblasts (64 Yr Donor)

 

Human iPSC-derived neural stem cells from patient donors
Cat. No.	Product Name	Donor
ax0111	Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (APOE4 HOM)	Fibroblasts (87 Yr Female)
ax0112	Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (PSEN1 L286V)	Fibroblasts (38 Yr Female)
ax0113	Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (PSEN1 M146L)	Fibroblasts (53 Yr Male)
ax0114	Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (PSEN1 A246E)	Fibroblasts (31 Yr Female)
ax0211	Human iPSC-Derived Neural Stem Cells - Huntington's Disease Patient	Fibroblasts (48 Yr Female)
ax0411	Human iPSC-Derived Neural Stem Cells - Epilepsy Patient	Fibroblasts (5 Mo Female)
ax1001	Human iPSC-Derived Neural Stem Cells - Trisomy X Patient	Fibroblasts (74 Yr Female)

 

axolGEM (genetically edited model) human iPSC-derived neural stem cells
Cat. No.	Product Name	Donor
ax0019	Human iPSC-Derived Neural Stem Cells (Female) Isogenic Control	Fibroblasts (64 Yr Donor)
ax0310	axolGEM iPSC-Derived Neural Stem Cells LRRK2 G2019S HOM	Fibroblasts (64 Yr Donor)
ax0311	axolGEM iPSC-Derived Neural Stem Cells LRRK2 G2019S HET	Fibroblasts (64 Yr Donor)
ax0320	axolGEM iPSC-Derived Neural Stem Cells MAPT R406W HOM	Fibroblasts (64 Yr Donor)
ax0321	axolGEM iPSC-Derived Neural Stem Cells MAPT R406W HET	Fibroblasts (64 Yr Donor)
ax0322	axolGEM iPSC-Derived Neural Stem Cells MAPT V337M HOM	Fibroblasts (64 Yr Donor)
ax0323	axolGEM iPSC-Derived Neural Stem Cells MAPT V337M HET	Fibroblasts (64 Yr Donor)
ax0324	axolGEM iPSC-Derived Neural Stem Cells MAPT P301L HOM	Fibroblasts (64 Yr Donor)
ax0325	axolGEM iPSC-Derived Neural Stem Cells MAPT P301L HET	Fibroblasts (64 Yr Donor)

Neural cell culture media and reagents
Cat. No.	Product Name	Quantity
ax0101	Neural Stem Cell Media & Reagent Bundle - Expansion and Synchronous Differentiation (System A)	Kit
ax0102	Neural Stem Cell Media & Reagent Bundle - Synchronous Differentiation (System B)	Kit
ax0103	Neural Stem Cell Media & Reagent Bundle - Expansion and Spontaneous Differentiation (System C)	Kit
ax0104	Neural Stem Cell Media & Reagent Bundle - Spontaneous Differentiation (System D)	Kit
ax0030-500	Neural Expansion-XF Medium	500 mL
ax0034-125	Neural Differentiation-XF Medium	125 mL
ax0032-500	Neural Maintenance-XF Medium	500 mL
ax0033	Neural Plating-XF Medium	30 mL
ax0041	SureBond	3 x 120 μL
ax0052	SureBond+ReadySet	3 x 120 μL & 2 x 10 mL
ax0053	SureBond-XF	1 mL
ax0044	Unlock	25 mL
ax0054	Unlock-XF	100 mL
ax0047	Recombinant Human FGF2	100 μg
ax0048	Recombinant Human EGF	100 μg
ax0051	ReadyFect	40 μL

• Transfection
• Neurite outgrowth
• Patch clamp
• Multi-electrode array (MEA)
• Transplatation

• Phase contrast

     Axol iPSC derived Neural Stem Cells grown in Neural Expansion XF Medium with the addition of EGF and EGF2. 

• Immunofluorescence

   

• Transfection

        ReadyFect leads to 30-40% transfection efficiency. The image demonstrates transfection results using 3 µL of
        ReadyFect / 1 µg of a GFP-encoding vector (pVectOZ-GFP). GFP expression was evaluated two days
        post-transfection.

• Neurite Outgrowth

• Patch Clamp

• MEA Analysis

   Protocol