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´Ù¾çÇÑ donorÀÇ cell¿¡ iPSC technology¸¦ µµÀÔÇÑ cerebral cortical neural stem cells (NSCs) ÀÔ´Ï´Ù. |
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AXOL |
Human iPSC-derived neural stem cells from healthy donors | ||
Cat. No. | Product Name | Donor |
ax0011 | Human iPSC-Derived Neural Stem Cells (Male) | Cord Blood CD34+ Cells |
ax0015 | Human iPSC-Derived Neural Stem Cells (Male) | Cord Blood CD34+ Cells |
ax0016 | Human iPSC-Derived Neural Stem Cells (Female) | Cord Blood CD34+ Cells |
ax0018 | Human iPSC-Derived Neural Stem Cells (Male) | Fibroblasts (74 Yr Donor) |
ax0019 | Human iPSC-Derived Neural Stem Cells (Female) | Fibroblasts (64 Yr Donor) |
Human iPSC-derived neural stem cells from patient donors | ||
Cat. No. | Product Name | Donor |
ax0111 | Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (APOE4 HOM) |
Fibroblasts (87 Yr Female) |
ax0112 | Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (PSEN1 L286V) |
Fibroblasts (38 Yr Female) |
ax0113 | Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (PSEN1 M146L) |
Fibroblasts (53 Yr Male) |
ax0114 | Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (PSEN1 A246E) |
Fibroblasts (31 Yr Female) |
ax0211 | Human iPSC-Derived Neural Stem Cells - Huntington's Disease Patient |
Fibroblasts (48 Yr Female) |
ax0411 | Human iPSC-Derived Neural Stem Cells - Epilepsy Patient |
Fibroblasts (5 Mo Female) |
ax1001 | Human iPSC-Derived Neural Stem Cells - Trisomy X Patient |
Fibroblasts (74 Yr Female) |
axolGEM (genetically edited model) human iPSC-derived neural stem cells | ||
Cat. No. | Product Name | Donor |
ax0019 | Human iPSC-Derived Neural Stem Cells (Female) Isogenic Control |
Fibroblasts (64 Yr Donor) |
ax0310 | axolGEM iPSC-Derived Neural Stem Cells LRRK2 G2019S HOM |
Fibroblasts (64 Yr Donor) |
ax0311 | axolGEM iPSC-Derived Neural Stem Cells LRRK2 G2019S HET |
Fibroblasts (64 Yr Donor) |
ax0320 | axolGEM iPSC-Derived Neural Stem Cells MAPT R406W HOM |
Fibroblasts (64 Yr Donor) |
ax0321 | axolGEM iPSC-Derived Neural Stem Cells MAPT R406W HET |
Fibroblasts (64 Yr Donor) |
ax0322 | axolGEM iPSC-Derived Neural Stem Cells MAPT V337M HOM |
Fibroblasts (64 Yr Donor) |
ax0323 | axolGEM iPSC-Derived Neural Stem Cells MAPT V337M HET |
Fibroblasts (64 Yr Donor) |
ax0324 | axolGEM iPSC-Derived Neural Stem Cells MAPT P301L HOM |
Fibroblasts (64 Yr Donor) |
ax0325 | axolGEM iPSC-Derived Neural Stem Cells MAPT P301L HET |
Fibroblasts (64 Yr Donor) |
Neural cell culture media and reagents | ||
Cat. No. | Product Name | Quantity |
ax0101 | Neural Stem Cell Media & Reagent Bundle - Expansion and Synchronous Differentiation (System A) |
Kit |
ax0102 | Neural Stem Cell Media & Reagent Bundle - Synchronous Differentiation (System B) |
Kit |
ax0103 | Neural Stem Cell Media & Reagent Bundle - Expansion and Spontaneous Differentiation (System C) |
Kit |
ax0104 | Neural Stem Cell Media & Reagent Bundle - Spontaneous Differentiation (System D) |
Kit |
ax0030-500 | Neural Expansion-XF Medium | 500 mL |
ax0034-125 | Neural Differentiation-XF Medium | 125 mL |
ax0032-500 | Neural Maintenance-XF Medium | 500 mL |
ax0033 | Neural Plating-XF Medium | 30 mL |
ax0041 | SureBond | 3 x 120 μL |
ax0052 | SureBond+ReadySet | 3 x 120 μL & 2 x 10 mL |
ax0053 | SureBond-XF | 1 mL |
ax0044 | Unlock | 25 mL |
ax0054 | Unlock-XF | 100 mL |
ax0047 | Recombinant Human FGF2 | 100 μg |
ax0048 | Recombinant Human EGF | 100 μg |
ax0051 | ReadyFect | 40 μL |
Axol iPSC derived Neural Stem Cells grown in Neural Expansion XF Medium with the addition of EGF and EGF2.
ReadyFect leads to 30-40% transfection efficiency. The image demonstrates transfection results using 3 µL of
ReadyFect / 1 µg of a GFP-encoding vector (pVectOZ-GFP). GFP expression was evaluated two days
post-transfection.
Protocol
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